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CLC number: Q813.1+1

On-line Access: 2018-02-06

Received: 2016-10-22

Revision Accepted: 2017-01-03

Crosschecked: 2018-01-08

Cited: 0

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Citations:  Bibtex RefMan EndNote GB/T7714


Yi-ying Zhao


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Journal of Zhejiang University SCIENCE B 2018 Vol.19 No.2 P.159-167


Toxicity testing of four silver nanoparticle-coated dental castings in 3-D LO2 cell cultures

Author(s):  Yi-ying Zhao, Qiang Chu, Xu-er Shi, Xiao-dong Zheng, Xiao-ting Shen, Yan-zhen Zhang

Affiliation(s):  Department of Food Science and Nutrition, Zhejiang Key Laboratory for Agro-Food Processing, Fuli Institute of Food Science, Hangzhou 310058, China; more

Corresponding email(s):   31300100694@zju.edu.cn, zyz85@hotmail.com

Key Words:  LO2 cell, 3-D model, Silver nanoparticles, Dental alloys, Toxicity test

Yi-ying Zhao, Qiang Chu, Xu-er Shi, Xiao-dong Zheng, Xiao-ting Shen, Yan-zhen Zhang. Toxicity testing of four silver nanoparticle-coated dental castings in 3-D LO2 cell cultures[J]. Journal of Zhejiang University Science B, 2018, 19(2): 159-167.

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journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

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%T Toxicity testing of four silver nanoparticle-coated dental castings in 3-D LO2 cell cultures
%A Yi-ying Zhao
%A Qiang Chu
%A Xu-er Shi
%A Xiao-dong Zheng
%A Xiao-ting Shen
%A Yan-zhen Zhang
%J Journal of Zhejiang University SCIENCE B
%V 19
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%P 159-167
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%D 2018
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1600482

T1 - Toxicity testing of four silver nanoparticle-coated dental castings in 3-D LO2 cell cultures
A1 - Yi-ying Zhao
A1 - Qiang Chu
A1 - Xu-er Shi
A1 - Xiao-dong Zheng
A1 - Xiao-ting Shen
A1 - Yan-zhen Zhang
J0 - Journal of Zhejiang University Science B
VL - 19
IS - 2
SP - 159
EP - 167
%@ 1673-1581
Y1 - 2018
PB - Zhejiang University Press & Springer
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DOI - 10.1631/jzus.B1600482

To address the controversial issue of the toxicity of dental alloys and silver nanoparticles in medical applications, an in vivo-like LO2 3-D model was constructed within polyvinylidene fluoride hollow fiber materials to mimic the microenvironment of liver tissue. The use of microscopy methods and the measurement of liver-specific functions optimized the model for best cell performances and also proved the superiority of the 3-D LO2 model when compared with the traditional monolayer model. toxicity tests were conducted using the newly constructed model, finding that four dental castings coated with silver nanoparticles were toxic to human hepatocytes after cell viability assays. In general, the toxicity of both the castings and the coated silver nanoparticles aggravated as time increased, yet the nanoparticles attenuated the general toxicity by preventing metal ion release, especially at high concentrations.




Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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