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CLC number: Q813.1+1

On-line Access: 2018-02-06

Received: 2016-10-22

Revision Accepted: 2017-01-03

Crosschecked: 2018-01-08

Cited: 0

Clicked: 3876

Citations:  Bibtex RefMan EndNote GB/T7714

 ORCID:

Yi-ying Zhao

https://orcid.org/0000-0002-9517-9669

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Journal of Zhejiang University SCIENCE B 2018 Vol.19 No.2 P.159-167

http://doi.org/10.1631/jzus.B1600482


Toxicity testing of four silver nanoparticle-coated dental castings in 3-D LO2 cell cultures


Author(s):  Yi-ying Zhao, Qiang Chu, Xu-er Shi, Xiao-dong Zheng, Xiao-ting Shen, Yan-zhen Zhang

Affiliation(s):  Department of Food Science and Nutrition, Zhejiang Key Laboratory for Agro-Food Processing, Fuli Institute of Food Science, Hangzhou 310058, China; more

Corresponding email(s):   31300100694@zju.edu.cn, zyz85@hotmail.com

Key Words:  LO2 cell, 3-D model, Silver nanoparticles, Dental alloys, Toxicity test


Yi-ying Zhao, Qiang Chu, Xu-er Shi, Xiao-dong Zheng, Xiao-ting Shen, Yan-zhen Zhang. Toxicity testing of four silver nanoparticle-coated dental castings in 3-D LO2 cell cultures[J]. Journal of Zhejiang University Science B, 2018, 19(2): 159-167.

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author="Yi-ying Zhao, Qiang Chu, Xu-er Shi, Xiao-dong Zheng, Xiao-ting Shen, Yan-zhen Zhang",
journal="Journal of Zhejiang University Science B",
volume="19",
number="2",
pages="159-167",
year="2018",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1600482"
}

%0 Journal Article
%T Toxicity testing of four silver nanoparticle-coated dental castings in 3-D LO2 cell cultures
%A Yi-ying Zhao
%A Qiang Chu
%A Xu-er Shi
%A Xiao-dong Zheng
%A Xiao-ting Shen
%A Yan-zhen Zhang
%J Journal of Zhejiang University SCIENCE B
%V 19
%N 2
%P 159-167
%@ 1673-1581
%D 2018
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1600482

TY - JOUR
T1 - Toxicity testing of four silver nanoparticle-coated dental castings in 3-D LO2 cell cultures
A1 - Yi-ying Zhao
A1 - Qiang Chu
A1 - Xu-er Shi
A1 - Xiao-dong Zheng
A1 - Xiao-ting Shen
A1 - Yan-zhen Zhang
J0 - Journal of Zhejiang University Science B
VL - 19
IS - 2
SP - 159
EP - 167
%@ 1673-1581
Y1 - 2018
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1600482


Abstract: 
To address the controversial issue of the toxicity of dental alloys and silver nanoparticles in medical applications, an in vivo-like LO2 3-D model was constructed within polyvinylidene fluoride hollow fiber materials to mimic the microenvironment of liver tissue. The use of microscopy methods and the measurement of liver-specific functions optimized the model for best cell performances and also proved the superiority of the 3-D LO2 model when compared with the traditional monolayer model. toxicity tests were conducted using the newly constructed model, finding that four dental castings coated with silver nanoparticles were toxic to human hepatocytes after cell viability assays. In general, the toxicity of both the castings and the coated silver nanoparticles aggravated as time increased, yet the nanoparticles attenuated the general toxicity by preventing metal ion release, especially at high concentrations.

LO2细胞3-D模型用于四种纳米银包裹的牙科合金材料毒性的检测

目的:应用体外三维模型模拟肝脏组织微环境,更真实地反映和评估纳米银材料和牙科合金对于人体的潜在毒性.
创新点:借助中空纤维管和胶原蛋白首次构建了LO2细胞三维聚集体,并将该模型应用到医用材料毒性的评价中.
方法:首先,采用扫描电镜观察中空纤维材料的孔径,确保营养物质的正常交换.然后,将混合有胶原蛋白的细胞悬液注入到中空纤维管的内胆,通过尿素氮和白蛋白检测,确定最佳细胞密度进行长期培养.在显微镜下观察细胞聚集体的形态,确保模型的成功建立.其次,应用水热法制作纳米银颗粒并将颗粒包裹到预先购买的合金材料上.最后,用不同牙科材料的浸提液培养细胞1、3和5天,通过MTT检测细胞死亡率,从而间接评价材料的毒性.
结论:中空纤维材料的表征结果显示该材料具有较好的耐热性和细胞粘附性,孔径大小适宜营养物质交换,可以应用到三维模型的构建中.通过白蛋白和尿素氮两个指标来评价三维模型的活性,发现每毫升5×104细胞的浓度最适宜细胞生长(图4).进一步模型评价表明,相比于传统单层培养的细胞,三维模型中的细胞能保持长期活力(图6)且可以在更短时间内对低药物浓度作出反应.

关键词:LO2细胞;三维模型;纳米银;牙科合金;毒性检测

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

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