CLC number: TS201.3
On-line Access: 2018-04-04
Received: 2017-01-20
Revision Accepted: 2017-05-15
Crosschecked: 2018-02-20
Cited: 0
Clicked: 4598
Yi-bo Zhu, Yan Xu, Li-mei Wang, Bin Qi. Biosynthesis of (R)-2-hydroxy-3-phenylpropionic acid using whole recombinant Escherichia coli cells in an aqueous/n-octane biphasic system[J]. Journal of Zhejiang University Science B, 2018, 19(4): 285-292.
@article{title="Biosynthesis of (R)-2-hydroxy-3-phenylpropionic acid using whole recombinant Escherichia coli cells in an aqueous/n-octane biphasic system",
author="Yi-bo Zhu, Yan Xu, Li-mei Wang, Bin Qi",
journal="Journal of Zhejiang University Science B",
volume="19",
number="4",
pages="285-292",
year="2018",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1700040"
}
%0 Journal Article
%T Biosynthesis of (R)-2-hydroxy-3-phenylpropionic acid using whole recombinant Escherichia coli cells in an aqueous/n-octane biphasic system
%A Yi-bo Zhu
%A Yan Xu
%A Li-mei Wang
%A Bin Qi
%J Journal of Zhejiang University SCIENCE B
%V 19
%N 4
%P 285-292
%@ 1673-1581
%D 2018
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1700040
TY - JOUR
T1 - Biosynthesis of (R)-2-hydroxy-3-phenylpropionic acid using whole recombinant Escherichia coli cells in an aqueous/n-octane biphasic system
A1 - Yi-bo Zhu
A1 - Yan Xu
A1 - Li-mei Wang
A1 - Bin Qi
J0 - Journal of Zhejiang University Science B
VL - 19
IS - 4
SP - 285
EP - 292
%@ 1673-1581
Y1 - 2018
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1700040
Abstract: (R)-2-hydroxy-3-phenylpropionic acid (PLA) is an ideal antimicrobial compound with broad-spectrum activity against a wide range of Gram-positive bacteria, some Gram-negative bacteria, and fungi. We studied the bioconversion of phenylpyruvate (PPA) to PLA using whole recombinant Escherichia coli cells in a series of buffer/organic solvent systems. Octane was found to be the best organic solvent. The optimum volume ratio of the water phase to the n-octane phase, conversion temperature, substrate concentration, and cell concentration were 6:4, 40 °C, 12.5 g/L, and 30 g/L wet cells, respectively. Under the optimized conditions, the average PLA productivity in the aqueous/ n-octane system was 30.69% higher than that in the aqueous system, and 32.31 g/L PLA was obtained with the use of a stirred reactor (2-L scale). Taken together, our findings indicated that PLA biosynthesis was more efficient in an aqueous/n-octane biphasic system than in a monophasic aqueous system. The proposed biphasic system is an effective strategy for enhancing PLA yield and the biosynthesis of its analogues.
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