CLC number: R845.2+1
On-line Access: 2024-08-27
Received: 2023-10-17
Revision Accepted: 2024-05-08
Crosschecked: 2020-07-10
Cited: 0
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Citations: Bibtex RefMan EndNote GB/T7714
Ke Ning, Zhen-biao Guan, Hong-tao Lu, Ning Zhang, Xue-jun Sun, Wen-wu Liu. Lung macrophages are involved in lung injury secondary to repetitive diving[J]. Journal of Zhejiang University Science B, 2020, 21(8): 646-656.
@article{title="Lung macrophages are involved in lung injury secondary to repetitive diving",
author="Ke Ning, Zhen-biao Guan, Hong-tao Lu, Ning Zhang, Xue-jun Sun, Wen-wu Liu",
journal="Journal of Zhejiang University Science B",
volume="21",
number="8",
pages="646-656",
year="2020",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1900687"
}
%0 Journal Article
%T Lung macrophages are involved in lung injury secondary to repetitive diving
%A Ke Ning
%A Zhen-biao Guan
%A Hong-tao Lu
%A Ning Zhang
%A Xue-jun Sun
%A Wen-wu Liu
%J Journal of Zhejiang University SCIENCE B
%V 21
%N 8
%P 646-656
%@ 1673-1581
%D 2020
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1900687
TY - JOUR
T1 - Lung macrophages are involved in lung injury secondary to repetitive diving
A1 - Ke Ning
A1 - Zhen-biao Guan
A1 - Hong-tao Lu
A1 - Ning Zhang
A1 - Xue-jun Sun
A1 - Wen-wu Liu
J0 - Journal of Zhejiang University Science B
VL - 21
IS - 8
SP - 646
EP - 656
%@ 1673-1581
Y1 - 2020
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1900687
Abstract: This study aimed to establish an animal model of decompression-induced lung injury (DILI) secondary to repetitive diving in mice and explore the role of macrophages in DILI and the protective effects of high-concentration hydrogen (HCH) on DILI. Mice were divided into three groups: control group, DILI group, and HCH group. Mice were exposed to hyperbaric air at 600 kPa for 60 min once daily for consecutive 3 d and then experienced decompression. In HCH group, mice were administered with HCH (66.7% hydrogen and 33.3% oxygen) for 60 min after each hyperbaric exposure. Pulmonary function tests were done 6 h after decompression; the blood was harvested for cell counting; the lung tissues were harvested for the detection of inflammatory cytokines, hematoxylin and eosin (HE) staining, and immunohistochemistry; western blotting and polymerase chain reaction (PCR) were done for the detection of markers for M1 and M2 macrophages. Our results showed that bubbles formed after decompression and repeated hyperbaric exposures significantly reduced the total lung volume and functional residual volume. Moreover, repetitive diving dramatically increased proinflammatory factors and increased the markers of both M1 and M2 macrophages. HCH inhalation improved lung function to a certain extent, and significantly reduced the pro-inflammatory factors. These effects were related to the reduction of M1 macrophages as well as the increase in M2 macrophages. This study indicates that repetitive diving damages lung function and activates lung macrophages, resulting in lung inflammation. HCH inhalation after each diving may be a promising strategy for the prevention of DILI.
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[26]List of electronic supplementary materials
[27]Materials and methods
[28]Fig. S1 Micro computed tomography scan for bubbles
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