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Journal of Zhejiang University SCIENCE B 2018 Vol.19 No.8 P.596-609

http://doi.org/10.1631/jzus.B1700388


An oriental melon 9-lipoxygenase gene CmLOX09 response to stresses, hormones, and signal substances


Author(s):  Li-Jun Ju, Chong Zhang, Jing-Jing Liao, Yue-Peng Li, Hong-Yan Qi

Affiliation(s):  Key Laboratory of Protected Horticulture of Education Ministry and Liaoning Province, College of Horticulture, Shenyang Agricultural University, Shenyang 110866, China

Corresponding email(s):   hyqiaaa@126.com

Key Words:  9-Lipoxygenase (9-LOX), Hydroperoxide lyase (HPL), Allene oxide synthase (AOS), Green leaf volatile, Jasmonic acid


Li-Jun Ju, Chong Zhang, Jing-Jing Liao, Yue-Peng Li, Hong-Yan Qi. An oriental melon 9-lipoxygenase gene CmLOX09 response to stresses, hormones, and signal substances[J]. Journal of Zhejiang University Science B, 2018, 19(8): 596-609.

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author="Li-Jun Ju, Chong Zhang, Jing-Jing Liao, Yue-Peng Li, Hong-Yan Qi",
journal="Journal of Zhejiang University Science B",
volume="19",
number="8",
pages="596-609",
year="2018",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1700388"
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%T An oriental melon 9-lipoxygenase gene CmLOX09 response to stresses, hormones, and signal substances
%A Li-Jun Ju
%A Chong Zhang
%A Jing-Jing Liao
%A Yue-Peng Li
%A Hong-Yan Qi
%J Journal of Zhejiang University SCIENCE B
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%N 8
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%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1700388

TY - JOUR
T1 - An oriental melon 9-lipoxygenase gene CmLOX09 response to stresses, hormones, and signal substances
A1 - Li-Jun Ju
A1 - Chong Zhang
A1 - Jing-Jing Liao
A1 - Yue-Peng Li
A1 - Hong-Yan Qi
J0 - Journal of Zhejiang University Science B
VL - 19
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SP - 596
EP - 609
%@ 1673-1581
Y1 - 2018
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1700388


Abstract: 
In plants, lipoxygenases (LOXs) play a crucial role in biotic and abiotic stresses. In our previous study, five 13-LOX genes of oriental melon were regulated by abiotic stress but it is unclear whether the 9-LOX is involved in biotic and abiotic stresses. The promoter analysis revealed that CmLOX09 (type of 9-LOX) has hormone elements, signal substances, and stress elements. We analyzed the expression of CmLOX09 and its downstream genes—CmHPL and CmAOS—in the leaves of four-leaf stage seedlings of the oriental melon cultivar “Yumeiren” under wound, hormone, and signal substances. CmLOX09, CmHPL, and CmAOS were all induced by wounding. CmLOX09 was induced by auxin (indole acetic acid, IAA) and gibberellins (GA3); however, CmHPL and CmAOS showed differential responses to IAA and GA3. CmLOX09, CmHPL, and CmAOS were all induced by hydrogen peroxide (H2O2) and methyl jasmonate (MeJA), while being inhibited by abscisic acid (ABA) and salicylic acid (SA). CmLOX09, CmHPL, and CmAOS were all induced by the powdery mildew pathogen Podosphaera xanthii. The content of 2-hexynol and 2-hexenal in leaves after MeJA treatment was significantly higher than that in the control. After infection with P. xanthii, the diseased leaves of the oriental melon were divided into four levels—levels 1, 2, 3, and 4. The content of jasmonic acid (JA) in the leaves of levels 1 and 3 was significantly higher than that in the level 0 leaves. In summary, the results suggested that CmLOX09 might play a positive role in the response to MeJA through the hydroperoxide lyase (HPL) pathway to produce C6 alcohols and aldehydes, and in the response to P. xanthii through the allene oxide synthase (AOS) pathway to form JA.

薄皮甜瓜9-脂氧合酶(9-LOX)类型的CmLOX09对逆境、激素和信号类物质的响应

目的:研究CmLOX09及其下游基因CmHPLCmAOS对逆境、激素和信号类物质的响应,进一步测定茉莉酸甲酯(MeJA)处理后叶片中绿叶挥发物的含量以及接种白粉病菌后叶片中茉莉酸含量的变化,探讨脂氧合酶(LOX)响应这两种胁迫的可能途径.
创新点:通过对CmLOX09启动子中顺式作用原件的分析预测,首次研究薄皮甜瓜9-LOX类型的CmLOX09对机械损伤、激素、信号类物质以及生物胁迫的响应.
方法:利用Plant CARE软件对CmLOX09启动子响应元件进行预测分析(图S1);利用荧光定量聚合酶链反应(qRT-PCR)技术分析甜瓜在机械损伤、激素、信号类物质以及生物胁迫处理后叶片中CmLOX09CmHPLCmAOS的表达模式;利用气相色谱-质谱连用仪(GC-MS)测定叶片中绿叶挥发物的含量(图4);利用高效液相色谱-串联质谱法(HPLC-MS/MS)分析和测定叶片中茉莉酸的含量(图6).
结论:本研究结果显示:CmLOX09参与机械损伤、激素、信号类物质及白粉病菌的防御反应(图1~3,5).9-LOX类型的CmLOX09可能通过氢过氧化物裂解酶(HPL)途径产生的绿叶挥发物(GLV)来响应MeJA(图4),并通过丙二烯合酶(AOS)途径产生的茉莉酸来响应真菌胁迫(图6).综上所述,9-LOX类型的CmLOX09可能在生物和非生物胁迫反应中起重要作用.

关键词:9-脂氧合酶;氢过氧化物裂解酶;丙二烯合酶;绿叶挥发物;茉莉酸

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

Reference

[1]Alam MM, Nahar K, Hasanuzzaman M, et al., 2014. Exogenous jasmonic acid modulates the physiology, antioxidant defense and glyoxalase systems in imparting drought stress tolerance in different Brassica species. Plant Biotechnol Rep, 8(3):279-293.

[2]Ameye M, Audenaert K, de Zutter N, et al., 2015. Priming of wheat with the green leaf volatile Z-3-hexenyl acetate enhances defense against Fusarium graminearum but boosts deoxynivalenol production. Plant Physiol, 167(4):1671-1684.

[3]Andreou A, Feussner I, 2009. Lipoxygenases—structure and reaction mechanism. Phytochemistry, 70(13-14):1504-1510.

[4]Bae KS, Rahimi S, Kim YJ, et al., 2016. Molecular characterization of lipoxygenase genes and their expression analysis against biotic and abiotic stresses in Panax ginseng. Eur J Plant Pathol, 145(2):331-343.

[5]Bell E, Creelman RA, Mullet JE, 1995. A chloroplast lipoxygenase is required for wound-induced jasmonic acid accumulation in Arabidopsis. Proc Natl Acad Sci USA, 92(19):8675-8679.

[6]Bhardwaj PK, Kaur J, Sobti RC, et al., 2011. Lipoxygenase in Caragana jubata responds to low temperature, abscisic acid, methyl jasmonate and salicylic acid. Gene, 483(1-2):49-53.

[7]Birkett MA, Campbell CAM, Chamberlain K, et al., 2000. New roles for cis-jasmone as an insect semiochemical and in plant defense. Proc Natl Acad Sci USA, 97(16):9329-9334.

[8]Brash AR, 1999. Lipoxygenases: occurrence, functions, catalysis, and acquisition of substrate. J Biol Chem, 274(34):23679-23682.

[9]Chauvin A, Lenglet A, Wolfender JL, et al., 2016. Paired hierarchical organization of 13-lipoxygenases in Arabidopsis. Plants, 5(2):16.

[10]Christensen SA, Kolomiets MV, 2011. The lipid language of plant–fungal interactions. Fungal Genet Biol, 48(1):4-14.

[11]Christensen SA, Nemchenko A, Borrego E, et al., 2013. The maize lipoxygenase, ZmLOX10, mediates green leaf volatile, jasmonate and herbivore-induced plant volatile production for defense against insect attack. Plant J, 74(1):59-73.

[12]Ding H, Lai JB, Wu Q, et al., 2016. Jasmonate complements the function of Arabidopsis lipoxygenase3 in salinity stress response. Plant Sci, 244:1-7.

[13]Durrant WE, Dong X, 2004. Systemic acquired resistance. Annu Rev Phytopathol, 42:185-209.

[14]Feussner I, Wasternack C, 2002. The lipoxygenase pathway. Annu Rev Plant Biol, 53:275-297.

[15]Gao XQ, Stumpe M, Feussner I, et al., 2008. A novel plastidial lipoxygenase of maize (Zea mays) ZmLOX6 encodes for a fatty acid hydroperoxide lyase and is uniquely regulated by phytohormones and pathogen infection. Planta, 227(2):491-503.

[16]Griffiths A, Barry C, Alpuche-Solis AG, et al., 1999. Ethylene and developmental signals regulate expression of lipoxygenase genes during tomato fruit ripening. J Exp Bot, 50(335):793-798.

[17]Guo XM, Stotz HU, 2007. Defense against Sclerotinia sclerotiorum in Arabidopsis is dependent on jasmonic acid, salicylic acid, and ethylene signaling. Mol Plant Microbe Interact, 20(11):1384-1395.

[18]Halitschke R, Baldwin LT, 2003. Antisense LOX expression increases herbivore performance by decreasing defense responses and inhibiting growth-related transcriptional reorganization in Nicotiana attenuata. Plant J, 36(6):794-807.

[19]Heitz T, Bergey DR, Ryan CA, 1997. A gene encoding a chloroplast-targeted lipoxygenase in tomato leaves is transiently induced by wounding, systemin, and methyl jasmonate. Plant Physiol, 114(3):1085-1093.

[20]Hou YL, Meng K, Han Y, et al., 2015. The persimmon 9-lipoxygenase gene DkLOX3 plays positive roles in both promoting senescence and enhancing tolerance to abiotic stress. Front Plant Sci, 6:1073.

[21]Hou YL, Bai QY, Meng K, et al., 2018. Overexpression of persimmon 9-lipoxygenase DkLOX3 confers resistance to Pseudomonas syringae pv. tomato DC3000 and Botrytis cinerea in Arabidopsis. Plant Growth Regul, 84(1):179-189.

[22]Hu TZ, Zeng H, Hu ZL, et al., 2013. Overexpression of the tomato 13-lipoxygenase gene TomloxD increases generation of endogenous jasmonic acid and resistance to Cladosporium fulvum and high temperature. Plant Mol Biol Rep, 31(5):1141-1149.

[23]Hu TZ, Hu ZL, Zeng H, et al., 2015. Tomato lipoxygenase D involved in the biosynthesis of jasmonic acid and tolerance to abiotic and biotic stress in tomato. Plant Biotechnol Rep, 9(1):37-45.

[24]Hwang IS, Hwang BK, 2010. The pepper 9-lipoxygenase gene CaLOX1 functions in defense and cell death responses to microbial pathogens. Plant Physiol, 152(2):948-967.

[25]Ivanov I, Heydeck D, Hofheinz K, et al., 2010. Molecular enzymology of lipoxygenases. Arch Biochem Biophys, 503(2):161-174.

[26]Kallenbach M, Gilardoni PA, Allmann S, et al., 2011. C12 derivatives of the hydroperoxide lyase pathway are produced by product recycling through lipoxygenase-2 in Nicotiana attenuata leaves. New Phytol, 191(4):1054-1068.

[27]Keereetaweep J, Blancaflor EB, Hornung E, et al., 2015. Lipoxygenase-derived 9-hydro(pero)xides of linoleoylethanolamide interact with ABA signaling to arrest root development during Arabidopsis seedling establishment. Plant J, 82(2):315-327.

[28]la Camera S, Balagué C, Göbel C, et al., 2009. The Arabidopsis Patatin-like protein 2 (PLP2) plays an essential role in cell death execution and differentially affects biosynthesis of oxylipins and resistance to pathogens. Mol Plant Microbe Interact, 22(4):469-481.

[29]León J, Royo J, Vancanneyt G, et al., 2002. Lipoxygenase H1 gene silencing reveals a specific role in supplying fatty acid hydroperoxides for aliphatic aldehyde production. J Biol Chem, 277(1):416-423.

[30]Liavonchanka A, Feussner I, 2006. Lipoxygenases: occurrence, functions and catalysis. Plant Physiol, 163(3):348-357.

[31]Lim CW, Han SW, Hwang IS, et al., 2015. The pepper lipoxygenase CaLOX1 plays a role in osmotic, drought and high salinity stress response. Plant Cell Physiol, 56(5):930-942.

[32]Liu JY, Zhang C, Shao Q, et al., 2016. Effects of abiotic stress and hormones on the expressions of five 13-CmLOXs and enzyme activity in oriental melon (Cucumis melo var. makuwa Makino). J Integr Agric, 15(2):326-338.

[33]Liu WN, Hildebrand DF, Grayburn WS, et al., 1991. Effects of exogenous auxins on expression of lipoxygenases in cultured soybean embryos. Plant Physiol, 97(3):969-976.

[34]Lv JY, Rao JP, Zhu YM, et al., 2014. Cloning and expression of lipoxygenase genes and enzyme activity in ripening persimmon fruit in response to GA and ABA treatments. Postharvest Biol Technol, 92:54-61.

[35]Maccarrone M, van Zadelhoff G, Veldink GA, et al., 2000. Early activation of lipoxygenase in lentil (Lens culinaris) root protoplasts by oxidative stress induces programmed cell death. Eur J Biochem, 267(16):5078-5084.

[36]Marmey P, Jalloul A, Alhamdia M, et al., 2007. The 9-lipoxygenase GhLOX1 gene is associated with the hypersensitive reaction of cotton Gossypium hirsutum to Xanthomonas campestris pv malvacearum. Plant Physiol Biochem, 45(8):596-606.

[37]Maschietto V, Marocco A, Malachova A, et al., 2015. Resistance to Fusarium verticillioides and fumonisin accumulation in maize inbred lines involves an earlier and enhanced expression of lipoxygenase (LOX) genes. J Plant Physiol, 188:9-18.

[38]Mostofa MG, Hossain MA, Fujita M, 2015. Trehalose pretreatment induces salt tolerance in rice (Oryza sativa L.) seedlings: oxidative damage and co-induction of antioxidant defense and glyoxalase systems. Protoplasma, 252(2):461-475.

[39]Neill SJ, Desikan R, Clarke A, et al., 2002. Hydrogen peroxide and nitric oxide as signalling molecules in plants. J Exp Bot, 53(372):1237-1247.

[40]Nemchenko A, Kunze S, Feussner I, et al., 2006. Duplicate maize 13-lipoxygenase genes are differentially regulated by circadian rhythm, cold stress, wounding, pathogen infection, and hormonal treatments. J Exp Bot, 57(14):3767-3779.

[41]Park JH, Halitschke R, Kim HB, et al., 2002. A knock-out mutation in allene oxide synthase results in male sterility and defective wound signal transduction in Arabidopsis due to a block in jasmonic acid biosynthesis. Plant J, 31(1):1-12.

[42]Porta H, Figueroa-Balderas RE, Rocha-Sosa M, 2008. Wounding and pathogen infection induce a chloroplast-targeted lipoxygenase in the common bean (Phaseolus vulgaris L.). Planta, 227(2):363-373.

[43]Rancé I, Fournier J, Esquerré-Tugayé MT, 1998. The incompatible interaction between Phytophthora parasitica var. nicotianae race 0 and tobacco is suppressed in transgenic plants expressing antisense lipoxygenase sequences. Proc Natl Acad Sci USA, 95(11):6554-6559.

[44]Sayegh-Alhamdia M, Marmey P, Jalloul A, et al., 2008. Association of lipoxygenase response with resistance of various cotton genotypes to the bacterial blight disease. J Phytopathol, 156(9):542-549.

[45]Shen JY, Tieman D, Jones JB, et al., 2014. A 13-lipoxygenase, TomloxC, is essential for synthesis of C5 flavour volatiles in tomato. J Exp Bot, 65(2):419-428.

[46]Tang YF, Zhang C, Cao SX, et al., 2015. The effect of CmLOXs on the production of volatile organic compounds in four aroma types of melon (Cucumis melo). PLoS ONE, 10(11):e0143567.

[47]Wang R, Shen WB, Liu LL, et al., 2008. A novel lipoxygenase gene from developing rice seeds confers dual position specificity and responds to wounding and insect attack. Plant Mol Biol, 66(4):401-414.

[48]Xin ZJ, Zhang LP, Zhang ZQ, et al., 2014. A tea hydroperoxide lyase gene, CsiHPL1, regulates tomato defense response against Prodenia litura (Fabricius) and Alternaria alternata f. sp. lycopersici by modulating green leaf volatiles (GLVs) release and jasmonic acid (JA) gene expression. Plant Mol Biol Rep, 32(1):62-69.

[49]Yan LH, Zhai QZ, Wei JN, et al., 2013. Role of tomato lipoxygenase D in wound-induced jasmonate biosynthesis and plant immunity to insect herbivores. PLoS Genet, 9(12):e1003964.

[50]Yang XY, Jiang WJ, Yu HJ, 2012. The expression profiling of the lipoxygenase (LOX) family genes during fruit development, abiotic stress and hormonal treatments in cucumber (Cucumis sativus L.). Int J Mol Sci, 13(2):2481-2500.

[51]Zhang C, Jin Y, Liu JY, et al., 2014. The phylogeny and expression profiles of the lipoxygenase (LOX) family genes in the melon (Cucumis melo L.) genome. Scientia Horticulturae, 170:94-102.

[52]Zhang C, Shao Q, Cao SX, et al., 2015. Effects of postharvest treatments on expression of three lipoxygenase genes in oriental melon (Cucumis melo var. makuwa Makino). Postharvest Biol Technol, 110:229-238.

[53]Zhang JH, Jia WS, Yang JC, et al., 2006. Role of ABA in integrating plant responses to drought and salt stresses. Field Crop Res, 97(1):111-119.

[54]Zhou GX, Qi JF, Ren N, et al., 2009. Silencing OsHI-LOX makes rice more susceptible to chewing herbivores, but enhances resistance to a phloem feeder. Plant J, 60(4):638-648.

[55]List of electronic supplementary materials

[56]Fig. S1 Cis-regulatory elements analysis of CmLOX09 promoter

[57]Fig. S2 Level of JA in oriental melon leaves after inoculation with Podosphaera xanthii analyzed with HPLC-MS/MS

[58]Table S1 Emission of GLVs in control and MeJA treatment leaves at 6, 72, and 168 h

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