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Journal of Zhejiang University SCIENCE B 2018 Vol.19 No.8 P.596-609


An oriental melon 9-lipoxygenase gene CmLOX09 response to stresses, hormones, and signal substances

Author(s):  Li-Jun Ju, Chong Zhang, Jing-Jing Liao, Yue-Peng Li, Hong-Yan Qi

Affiliation(s):  Key Laboratory of Protected Horticulture of Education Ministry and Liaoning Province, College of Horticulture, Shenyang Agricultural University, Shenyang 110866, China

Corresponding email(s):   hyqiaaa@126.com

Key Words:  9-Lipoxygenase (9-LOX), Hydroperoxide lyase (HPL), Allene oxide synthase (AOS), Green leaf volatile, Jasmonic acid

Li-Jun Ju, Chong Zhang, Jing-Jing Liao, Yue-Peng Li, Hong-Yan Qi. An oriental melon 9-lipoxygenase gene CmLOX09 response to stresses, hormones, and signal substances[J]. Journal of Zhejiang University Science B, 2018, 19(8): 596-609.

@article{title="An oriental melon 9-lipoxygenase gene CmLOX09 response to stresses, hormones, and signal substances",
author="Li-Jun Ju, Chong Zhang, Jing-Jing Liao, Yue-Peng Li, Hong-Yan Qi",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T An oriental melon 9-lipoxygenase gene CmLOX09 response to stresses, hormones, and signal substances
%A Li-Jun Ju
%A Chong Zhang
%A Jing-Jing Liao
%A Yue-Peng Li
%A Hong-Yan Qi
%J Journal of Zhejiang University SCIENCE B
%V 19
%N 8
%P 596-609
%@ 1673-1581
%D 2018
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1700388

T1 - An oriental melon 9-lipoxygenase gene CmLOX09 response to stresses, hormones, and signal substances
A1 - Li-Jun Ju
A1 - Chong Zhang
A1 - Jing-Jing Liao
A1 - Yue-Peng Li
A1 - Hong-Yan Qi
J0 - Journal of Zhejiang University Science B
VL - 19
IS - 8
SP - 596
EP - 609
%@ 1673-1581
Y1 - 2018
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1700388

In plants, lipoxygenases (LOXs) play a crucial role in biotic and abiotic stresses. In our previous study, five 13-LOX genes of oriental melon were regulated by abiotic stress but it is unclear whether the 9-LOX is involved in biotic and abiotic stresses. The promoter analysis revealed that CmLOX09 (type of 9-LOX) has hormone elements, signal substances, and stress elements. We analyzed the expression of CmLOX09 and its downstream genes—CmHPL and CmAOS—in the leaves of four-leaf stage seedlings of the oriental melon cultivar “Yumeiren” under wound, hormone, and signal substances. CmLOX09, CmHPL, and CmAOS were all induced by wounding. CmLOX09 was induced by auxin (indole acetic acid, IAA) and gibberellins (GA3); however, CmHPL and CmAOS showed differential responses to IAA and GA3. CmLOX09, CmHPL, and CmAOS were all induced by hydrogen peroxide (H2O2) and methyl jasmonate (MeJA), while being inhibited by abscisic acid (ABA) and salicylic acid (SA). CmLOX09, CmHPL, and CmAOS were all induced by the powdery mildew pathogen Podosphaera xanthii. The content of 2-hexynol and 2-hexenal in leaves after MeJA treatment was significantly higher than that in the control. After infection with P. xanthii, the diseased leaves of the oriental melon were divided into four levels—levels 1, 2, 3, and 4. The content of jasmonic acid (JA) in the leaves of levels 1 and 3 was significantly higher than that in the level 0 leaves. In summary, the results suggested that CmLOX09 might play a positive role in the response to MeJA through the hydroperoxide lyase (HPL) pathway to produce C6 alcohols and aldehydes, and in the response to P. xanthii through the allene oxide synthase (AOS) pathway to form JA.


方法:利用Plant CARE软件对CmLOX09启动子响应元件进行预测分析(图S1);利用荧光定量聚合酶链反应(qRT-PCR)技术分析甜瓜在机械损伤、激素、信号类物质以及生物胁迫处理后叶片中CmLOX09CmHPLCmAOS的表达模式;利用气相色谱-质谱连用仪(GC-MS)测定叶片中绿叶挥发物的含量(图4);利用高效液相色谱-串联质谱法(HPLC-MS/MS)分析和测定叶片中茉莉酸的含量(图6).


Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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[55]List of electronic supplementary materials

[56]Fig. S1 Cis-regulatory elements analysis of CmLOX09 promoter

[57]Fig. S2 Level of JA in oriental melon leaves after inoculation with Podosphaera xanthii analyzed with HPLC-MS/MS

[58]Table S1 Emission of GLVs in control and MeJA treatment leaves at 6, 72, and 168 h

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