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CLC number: O652.1

On-line Access: 2018-09-04

Received: 2017-10-11

Revision Accepted: 2017-12-04

Crosschecked: 2018-08-31

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Journal of Zhejiang University SCIENCE B 2018 Vol.19 No.9 P.726-734


An anti-passivation ink for the preparation of electrodes for use in electrochemical immunoassays

Author(s):  Qi-Qi Zheng, Yuan-Chao Lu, Zun-Zhong Ye, Jian-Feng Ping, Jian Wu, Yi-Bin Ying

Affiliation(s):  College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China; more

Corresponding email(s):   wujian69@zju.edu.cn

Key Words:  Electrochemical immunoassay, Electrode ink, Anti-passivation, Ionic liquid, p-Nitrophenol

Qi-Qi Zheng, Yuan-Chao Lu, Zun-Zhong Ye, Jian-Feng Ping, Jian Wu, Yi-Bin Ying. An anti-passivation ink for the preparation of electrodes for use in electrochemical immunoassays[J]. Journal of Zhejiang University Science B, 2018, 19(9): 726-734.

@article{title="An anti-passivation ink for the preparation of electrodes for use in electrochemical immunoassays",
author="Qi-Qi Zheng, Yuan-Chao Lu, Zun-Zhong Ye, Jian-Feng Ping, Jian Wu, Yi-Bin Ying",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T An anti-passivation ink for the preparation of electrodes for use in electrochemical immunoassays
%A Qi-Qi Zheng
%A Yuan-Chao Lu
%A Zun-Zhong Ye
%A Jian-Feng Ping
%A Jian Wu
%A Yi-Bin Ying
%J Journal of Zhejiang University SCIENCE B
%V 19
%N 9
%P 726-734
%@ 1673-1581
%D 2018
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1700510

T1 - An anti-passivation ink for the preparation of electrodes for use in electrochemical immunoassays
A1 - Qi-Qi Zheng
A1 - Yuan-Chao Lu
A1 - Zun-Zhong Ye
A1 - Jian-Feng Ping
A1 - Jian Wu
A1 - Yi-Bin Ying
J0 - Journal of Zhejiang University Science B
VL - 19
IS - 9
SP - 726
EP - 734
%@ 1673-1581
Y1 - 2018
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1700510

p-Nitrophenylphosphate (PNPP) is usually employed as the substrate for enzyme-linked immunosorbent assays. p-Nitrophenol (PNP), the product of PNPP, with the catalyst alkaline phosphatase (ALP), will passivate an electrode, which limits applications in electrochemical analysis. A novel anti-passivation ink used in the preparation of a graphene/ionic liquid/chitosan composited (rGO/IL/Chi) electrode is proposed to solve the problem. The anti-passivation electrode was fabricated by directly writing the graphene-ionic liquid-chitosan composite on a single-side conductive gold strip. A glassy carbon electrode, a screen-printed electrode, and a graphene-chitosan composite-modified screen-printed electrode were investigated for comparison. Scanning electron microscopy was used to characterize the surface structure of the four different electrodes and cyclic voltammetry was carried out to compare their performance. The results showed that the rGO/IL/Chi electrode had the best performance according to its low peak potential and large peak current. Amperometric responses of the different electrodes to PNP proved that only the rGO/IL/Chi electrode was capable of anti-passivation. The detection of cardiac troponin I was used as a test example for electrochemical immunoassay. Differential pulse voltammetry was performed to detect cardiac troponin I and obtain a calibration curve. The limit of detection was 0.05 ng/ml.


方法:制备rGO/IL/Chi复合浆料,涂布于单面导电金箔上,75 °C,10 min,将其作为工作电极;背面涂布自制的银浆,作为参比电极和对电极;0.03 g/ml壳聚糖溶液作为绝缘浆料.
结论:本研究表明,在比较商品化的玻碳电极、自制丝网印刷电极、石墨烯修饰的丝网印刷电极和rGO/IL/Chi电极对PNP的循环伏安响应时,rGO/IL/Chi电极具有最大的峰电流响应和最小的峰电位.同时,对四种电极进行性能表征时,rGO/IL/Chi电极具有最小的峰电位差和最大的峰电流.这表明rGO/IL/Chi电极具有较好的电化学性能,且对PNP有较大的响应.比较四种电极对PNP的安培响应之后,发现只有rGO/IL/Chi电极具有抗钝化作用.可将该电极用于肌钙蛋白I的检测,其检测限为0.05 ng/ml.


Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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[28]List of electronic supplementary materials

[29]Fig. S1 Absorbance of test solution with different kinds of blocking solutions

[30]Fig. S2 Absorbance of test solution with different concentrations of capture antibody

[31]Fig. S3 Absorbance of test solution with different concentrations of ALP-conjugated antibody

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