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CLC number: O652.1

On-line Access: 2018-09-04

Received: 2017-10-11

Revision Accepted: 2017-12-04

Crosschecked: 2018-08-31

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Journal of Zhejiang University SCIENCE B 2018 Vol.19 No.9 P.726-734

http://doi.org/10.1631/jzus.B1700510


An anti-passivation ink for the preparation of electrodes for use in electrochemical immunoassays


Author(s):  Qi-Qi Zheng, Yuan-Chao Lu, Zun-Zhong Ye, Jian-Feng Ping, Jian Wu, Yi-Bin Ying

Affiliation(s):  College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China; more

Corresponding email(s):   wujian69@zju.edu.cn

Key Words:  Electrochemical immunoassay, Electrode ink, Anti-passivation, Ionic liquid, p-Nitrophenol


Qi-Qi Zheng, Yuan-Chao Lu, Zun-Zhong Ye, Jian-Feng Ping, Jian Wu, Yi-Bin Ying. An anti-passivation ink for the preparation of electrodes for use in electrochemical immunoassays[J]. Journal of Zhejiang University Science B, 2018, 19(9): 726-734.

@article{title="An anti-passivation ink for the preparation of electrodes for use in electrochemical immunoassays",
author="Qi-Qi Zheng, Yuan-Chao Lu, Zun-Zhong Ye, Jian-Feng Ping, Jian Wu, Yi-Bin Ying",
journal="Journal of Zhejiang University Science B",
volume="19",
number="9",
pages="726-734",
year="2018",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1700510"
}

%0 Journal Article
%T An anti-passivation ink for the preparation of electrodes for use in electrochemical immunoassays
%A Qi-Qi Zheng
%A Yuan-Chao Lu
%A Zun-Zhong Ye
%A Jian-Feng Ping
%A Jian Wu
%A Yi-Bin Ying
%J Journal of Zhejiang University SCIENCE B
%V 19
%N 9
%P 726-734
%@ 1673-1581
%D 2018
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1700510

TY - JOUR
T1 - An anti-passivation ink for the preparation of electrodes for use in electrochemical immunoassays
A1 - Qi-Qi Zheng
A1 - Yuan-Chao Lu
A1 - Zun-Zhong Ye
A1 - Jian-Feng Ping
A1 - Jian Wu
A1 - Yi-Bin Ying
J0 - Journal of Zhejiang University Science B
VL - 19
IS - 9
SP - 726
EP - 734
%@ 1673-1581
Y1 - 2018
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1700510


Abstract: 
p-Nitrophenylphosphate (PNPP) is usually employed as the substrate for enzyme-linked immunosorbent assays. p-Nitrophenol (PNP), the product of PNPP, with the catalyst alkaline phosphatase (ALP), will passivate an electrode, which limits applications in electrochemical analysis. A novel anti-passivation ink used in the preparation of a graphene/ionic liquid/chitosan composited (rGO/IL/Chi) electrode is proposed to solve the problem. The anti-passivation electrode was fabricated by directly writing the graphene-ionic liquid-chitosan composite on a single-side conductive gold strip. A glassy carbon electrode, a screen-printed electrode, and a graphene-chitosan composite-modified screen-printed electrode were investigated for comparison. Scanning electron microscopy was used to characterize the surface structure of the four different electrodes and cyclic voltammetry was carried out to compare their performance. The results showed that the rGO/IL/Chi electrode had the best performance according to its low peak potential and large peak current. Amperometric responses of the different electrodes to PNP proved that only the rGO/IL/Chi electrode was capable of anti-passivation. The detection of cardiac troponin I was used as a test example for electrochemical immunoassay. Differential pulse voltammetry was performed to detect cardiac troponin I and obtain a calibration curve. The limit of detection was 0.05 ng/ml.

一种用于电化学免疫分析的抗钝化的电极浆料

目的:酶联免疫分析常用底物对硝基苯磷酸盐分解后产生对硝基苯酚(PNP),该物质对电极具有钝化作用,限制了该体系在电化学免疫分析领域的应用.因此,需要开发一种具有抗钝化作用的电极浆料.
创新点:石墨烯-子液体-壳聚糖(rGO/IL/Chi)电极浆料不仅具有抗钝化作用,且拥有良好的电化学性能,制备过程中无需使用任何有机溶剂,安全环保.
方法:制备rGO/IL/Chi复合浆料,涂布于单面导电金箔上,75 °C,10 min,将其作为工作电极;背面涂布自制的银浆,作为参比电极和对电极;0.03 g/ml壳聚糖溶液作为绝缘浆料.
结论:本研究表明,在比较商品化的玻碳电极、自制丝网印刷电极、石墨烯修饰的丝网印刷电极和rGO/IL/Chi电极对PNP的循环伏安响应时,rGO/IL/Chi电极具有最大的峰电流响应和最小的峰电位.同时,对四种电极进行性能表征时,rGO/IL/Chi电极具有最小的峰电位差和最大的峰电流.这表明rGO/IL/Chi电极具有较好的电化学性能,且对PNP有较大的响应.比较四种电极对PNP的安培响应之后,发现只有rGO/IL/Chi电极具有抗钝化作用.可将该电极用于肌钙蛋白I的检测,其检测限为0.05 ng/ml.

关键词:电化学免疫分析;电极浆料;抗钝化;离子液体;对硝基苯酚

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

Reference

[1]Aneesh K, Berchmans S, 2017. Highly selective sensing of dopamine using carbon nanotube ink doped with anionic surfactant modified disposable paper electrode. J Solid State Electrochem, 21(5):1263-1271.

[2]Bansod B, Kumar T, Thakur R, et al., 2017. A review on various electrochemical techniques for heavy metal ions detection with different sensing platforms. Biosens Bioelectron, 94:443-455.

[3]Budnikov HC, Shirokova VI, 2013. Term “Nano” in electroanalysis: a trendy prefix or a new stage of its development? J Anal Chem, 68(8):663-670.

[4]Cinti S, Basso M, Moscone D, et al., 2017. A paper-based nanomodified electrochemical biosensor for ethanol detection in beers. Anal Chim Acta, 960:123-130.

[5]Dinesh B, Saraswathi R, Kumar AS, 2017. Water based homogenous carbon ink modified electrode as an efficient sensor system for simultaneous detection of ascorbic acid, dopamine and uric acid. Electrochim Acta, 233:92-104.

[6]Ghosale A, Shrivas K, Shankar R, et al., 2017. Low-cost paper electrode fabricated by direct writing with silver nanoparticle-based ink for detection of hydrogen peroxide in wastewater. Anal Chem, 89(1):776-782.

[7]González-Sánchez MI, Valero E, Compton RG, 2016. Iodine mediated electrochemical detection of thiols in plant extracts using platinum screen-printed electrodes. Sens Actuators B Chem, 236:1-7.

[8]Idris A, Saleh TA, Sanhoob MA, et al., 2017. Electrochemical detection of thiocyanate using phosphate-modified zeolite carbon paste electrodes. J Taiwan Inst Chem Eng, 72: 236-243.

[9]Kuila T, Bose S, Khanra P, et al., 2011. Recent advances in graphene-based biosensors. Biosens Bioelectron, 26(12):4637-4648.

[10]Li S, Zhang Q, Lu YL, et al., 2017. One step electrochemical deposition and reduction of graphene oxide on screen printed electrodes for impedance detection of glucose. Sens Actuators B Chem, 244:290-298.

[11]Li SJ, Zuo Y, Huang WF, 2017. Establishment of a reference interval for high-sensitivity cardiac troponin I in healthy adults from the Sichuan area. Medicine, 96(14):e6252.

[12]Ma XY, Chao MY, Wang ZX, et al., 2012. Electrochemical detection of dopamine in the presence of epinephrine, uric acid and ascorbic acid using a graphene-modified electrode. Anal Methods, 4(6):1687-1692.

[13]Molazemhosseini A, Magagnin L, Vena P, et al., 2017. Single-use nonenzymatic glucose biosensor based on CuO nanoparticles ink printed on thin film gold electrode by micro-plotter technology. J Electroanal Chem, 789:50-57.

[14]Nesakumar N, Sethuraman S, Krishnan UM, et al., 2016. Electrochemical acetylcholinesterase biosensor based on ZnO nanocuboids modified platinum electrode for the detection of carbosulfan in rice. Biosens Bioelectron, 77: 1070-1077.

[15]Ping JF, Wu J, Ying YB, 2010. Development of an ionic liquid modified screen-printed graphite electrode and its sensing in determination of dopamine. Electrochem Commun, 12(12):1738-1741.

[16]Ping JF, Wang YX, Ying YB, et al., 2012a. Application of electrochemically reduced graphene oxide on screen-printed ion-selective electrode. Anal Chem, 84(7):3473-3479.

[17]Ping JF, Wu J, Wang YX, et al., 2012b. Simultaneous determination of ascorbic acid, dopamine and uric acid using high-performance screen-printed graphene electrode. Biosens Bioelectron, 34(1):70-76.

[18]Preechaworapun A, Dai Z, Xiang Y, et al., 2008. Investigation of the enzyme hydrolysis products of the substrates of alkaline phosphatase in electrochemical immunosensing. Talanta, 76(2):424-431.

[19]Saita T, Yamamoto Y, Hosoya K, et al., 2017. An ultra-specific and sensitive sandwich ELISA for imatinib using two anti-imatinib antibodies. Anal Chim Acta, 969:72-78.

[20]Sajid M, Nazal MK, Mansha M, et al., 2016. Chemically modified electrodes for electrochemical detection of dopamine in the presence of uric acid and ascorbic acid: a review. TrAC Trend Anal Chem, 76:15-29.

[21]Secor EB, Ahn BY, Gao TZ, et al., 2015. Rapid and versatile photonic annealing of graphene inks for flexible printed electronics. Adv Mater, 27(42):6683-6688.

[22]Tang WZ, Wu J, 2014. Amperometric determination of organophosphorus pesticide by silver electrode using an acetylcholinesterase inhibition method. Anal Methods, 6(3):924-929.

[23]Tang WZ, Zhou JZ, Yang QQ, et al., 2014. Determination of methyl parathion by solid-phase extraction on an ionic liquid-carbon nanotube composite electrode. Anal Methods, 6(15):5886-5890.

[24]Wang YX, Ping JF, Ye ZZ, et al., 2013. Impedimetric immunosensor based on gold nanoparticles modified graphene paper for label-free detection of Escherichia coli O157:H7. Biosens Bioelectron, 49:492-498.

[25]Yang SX, Chen YC, Nicolini L, et al., 2015. “Cut-and-Paste” manufacture of multiparametric epidermal sensor systems. Adv Mater, 27(41):6423-6430.

[26]Yu XW, Sheng KX, Shi GQ, 2014. A three-dimensional interpenetrating electrode of reduced graphene oxide for selective detection of dopamine. Analyst, 139(18):4525-4531.

[27]Zheng QQ, Yu YH, Fan K, et al., 2016. A nano-silver enzyme electrode for organophosphorus pesticide detection. Anal Bioanal Chem, 408(21):5819-5827.

[28]List of electronic supplementary materials

[29]Fig. S1 Absorbance of test solution with different kinds of blocking solutions

[30]Fig. S2 Absorbance of test solution with different concentrations of capture antibody

[31]Fig. S3 Absorbance of test solution with different concentrations of ALP-conjugated antibody

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