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Journal of Zhejiang University SCIENCE B 2018 Vol.19 No.11 P.844-852

http://doi.org/10.1631/jzus.B1800076


Effect of a combination of astaxanthin supplementation, heat stress, and intermittent reloading on satellite cells during disuse muscle atrophy


Author(s):  Toshinori Yoshihara, Takao Sugiura, Nobuyuki Miyaji, Yuki Yamamoto, Tsubasa Shibaguchi, Ryo Kakigi, Hisashi Naito, Katsumasa Goto, Daijiro Ohmori, Toshitada Yoshioka

Affiliation(s):  Graduate School of Health and Sports Science, Juntendo University, Inzai, Chiba 270-1695, Japan; more

Corresponding email(s):   t-yoshih@juntendo.ac.jp

Key Words:  Antioxidant astaxanthin, Disuse muscle atrophy, Heat stress, Satellite cell


Toshinori Yoshihara, Takao Sugiura, Nobuyuki Miyaji, Yuki Yamamoto, Tsubasa Shibaguchi, Ryo Kakigi, Hisashi Naito, Katsumasa Goto, Daijiro Ohmori, Toshitada Yoshioka. Effect of a combination of astaxanthin supplementation, heat stress, and intermittent reloading on satellite cells during disuse muscle atrophy[J]. Journal of Zhejiang University Science B, 2018, 19(11): 844-852.

@article{title="Effect of a combination of astaxanthin supplementation, heat stress, and intermittent reloading on satellite cells during disuse muscle atrophy",
author="Toshinori Yoshihara, Takao Sugiura, Nobuyuki Miyaji, Yuki Yamamoto, Tsubasa Shibaguchi, Ryo Kakigi, Hisashi Naito, Katsumasa Goto, Daijiro Ohmori, Toshitada Yoshioka",
journal="Journal of Zhejiang University Science B",
volume="19",
number="11",
pages="844-852",
year="2018",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1800076"
}

%0 Journal Article
%T Effect of a combination of astaxanthin supplementation, heat stress, and intermittent reloading on satellite cells during disuse muscle atrophy
%A Toshinori Yoshihara
%A Takao Sugiura
%A Nobuyuki Miyaji
%A Yuki Yamamoto
%A Tsubasa Shibaguchi
%A Ryo Kakigi
%A Hisashi Naito
%A Katsumasa Goto
%A Daijiro Ohmori
%A Toshitada Yoshioka
%J Journal of Zhejiang University SCIENCE B
%V 19
%N 11
%P 844-852
%@ 1673-1581
%D 2018
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1800076

TY - JOUR
T1 - Effect of a combination of astaxanthin supplementation, heat stress, and intermittent reloading on satellite cells during disuse muscle atrophy
A1 - Toshinori Yoshihara
A1 - Takao Sugiura
A1 - Nobuyuki Miyaji
A1 - Yuki Yamamoto
A1 - Tsubasa Shibaguchi
A1 - Ryo Kakigi
A1 - Hisashi Naito
A1 - Katsumasa Goto
A1 - Daijiro Ohmori
A1 - Toshitada Yoshioka
J0 - Journal of Zhejiang University Science B
VL - 19
IS - 11
SP - 844
EP - 852
%@ 1673-1581
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PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1800076


Abstract: 
We examined the effect of a combination of astaxanthin (AX) supplementation, repeated heat stress, and intermittent reloading (IR) on satellite cells in unloaded rat soleus muscles. Forty-nine male Wistar rats (8-week-old) were divided into control, hind-limb unweighting (HU), IR during HU, IR with AX supplementation, IR with repeated heat stress (41.0–41.5 °C for 30 min), and IR with AX supplementation and repeated heat stress groups. After the experimental period, the antigravitational soleus muscle was analyzed using an immunohistochemical technique. Our results revealed that the combination of dietary AX supplementation and heat stress resulted in protection against disuse muscle atrophy in the soleus muscle. This protective effect may be partially due to a higher satellite cell number in the atrophied soleus muscle in the IR/AX/heat stress group compared with the numbers found in the other groups. We concluded that the combination treatment with dietary AX supplementation and repeated heat stress attenuates soleus muscle atrophy, in part by increasing the number of satellite cells.

虾青素摄入、热应激和间歇式再负荷的联合处理对废用性肌萎缩的肌卫星细胞的影响

目的:探讨透过虾青素摄入、热应激和间歇式再负荷的不同处理方式及其联合处理,对于尾部悬吊后大鼠比目鱼肌卫星细胞的影响.
创新点:利用虾青素摄入、热应激与间歇式再负荷的不同方式,探讨预防大鼠比目鱼肌废用性肌萎缩的有效处理.
方法:将49只雄性Wistar大鼠分成对照组、吊尾组、间歇式再负荷组、虾青素摄入组、热应激组和虾青素摄入+热应激组.摘取7天尾部悬吊后的大鼠的比目鱼肌,使用免疫组织化学技术,分析肌卫星细胞的激活程度.
结论:虾青素摄入、热应激与间歇式再负荷的不同方式的联合处理能减少比目鱼肌废用性肌萎缩,其部分效果可能是由于肌卫星细胞数量的增加所致.

关键词:虾青素;废用性肌萎缩;热应激;肌卫星细胞

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

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