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CLC number: S823

On-line Access: 2015-06-08

Received: 2014-11-30

Revision Accepted: 2015-05-23

Crosschecked: 2015-05-25

Cited: 7

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Citations:  Bibtex RefMan EndNote GB/T7714


Nan Zheng


Hai-na Gao


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Journal of Zhejiang University SCIENCE B 2015 Vol.16 No.6 P.560-572


Leucine and histidine independently regulate milk protein synthesis in bovine mammary epithelial cells via mTOR signaling pathway

Author(s):  Hai-na Gao, Han Hu, Nan Zheng, Jia-qi Wang

Affiliation(s):  Ministry of Agriculture-Milk Risk Assessment Laboratory, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China; more

Corresponding email(s):   zhengnan_1980@126.com

Key Words:  Bovine mammary epithelial cells, Leucine, Histidine, Western blotting, mTOR, Casein

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Hai-na Gao, Han Hu, Nan Zheng, Jia-qi Wang. Leucine and histidine independently regulate milk protein synthesis in bovine mammary epithelial cells via mTOR signaling pathway[J]. Journal of Zhejiang University Science B, 2015, 16(6): 560-572.

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author="Hai-na Gao, Han Hu, Nan Zheng, Jia-qi Wang",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Leucine and histidine independently regulate milk protein synthesis in bovine mammary epithelial cells via mTOR signaling pathway
%A Hai-na Gao
%A Han Hu
%A Nan Zheng
%A Jia-qi Wang
%J Journal of Zhejiang University SCIENCE B
%V 16
%N 6
%P 560-572
%@ 1673-1581
%D 2015
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1400337

T1 - Leucine and histidine independently regulate milk protein synthesis in bovine mammary epithelial cells via mTOR signaling pathway
A1 - Hai-na Gao
A1 - Han Hu
A1 - Nan Zheng
A1 - Jia-qi Wang
J0 - Journal of Zhejiang University Science B
VL - 16
IS - 6
SP - 560
EP - 572
%@ 1673-1581
Y1 - 2015
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1400337

The aim of this study is to investigate the effects of leucine (Leu) and histidine (His) on the expression of both the mammalian target of rapamycin (mTOR) signaling pathway-related proteins and caseins in immortalized bovine mammary epithelial cells (CMEC-H), using a single supplement through western blotting. The Earle’s balanced salt solution (EBSS) was set as the control group and other treatment groups, based on the EBSS, were added with different concentrations of Leu or His, respectively. The results showed that, compared with the control group, the expression of caseins and the phosphorylation of mTOR (Ser2481), Raptor (Ser792), eIF4E (Ser209), and eEF2 (Thr56) increased with the Leu concentrations ranging from 0.45 to 10.80 mmol/L (P<0.01). The P-4EBP1 (Thr37) at 10.80 mmol/L Leu, and P-RPS6 (Ser235/236) at 5.40 to 10.80 mmol/L Leu all decreased. Similarly, the His supplementation from 0.15 to 9.60 mmol/L increased the expression of αs2-casein, β-casein, κ-casein, P-mTOR (Ser2481), P-Raptor (Ser792), P-S6K1 (Thr389), P-4EBP1 (Thr37), P-eIF4E (Ser209), and P-eEF2 (Thr56) (P<0.01) in CMEC-H, whereas the αs1-casein expression was only reduced at 9.60 mmol/L His, G protein β subunit-like protein (GβL) at 0.15 and 9.60 mmol/L His, and P-RPS6 at 4.80 to 9.60 mmol/L His. Our linear regression model assay suggested that the αs1-casein expression was positively correlated with P-mTOR (P<0.01), P-S6K1 (P<0.01), and P-eEF2 (P<0.01) for the addition of Leu, while the expressions of β-casein (P<0.01) and κ-casein (P<0.01) were positively correlated with P-eEF2 for the addition of His. In conclusion, the milk protein synthesis was up-regulated through activation of the mTOR pathway with the addition of Leu and His in CMEC-H.




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