Full Text:   <1136>

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CLC number: Q615

On-line Access: 2019-11-21

Received: 2019-08-09

Revision Accepted: 2019-10-09

Crosschecked: 2019-10-23

Cited: 0

Clicked: 2273

Citations:  Bibtex RefMan EndNote GB/T7714

 ORCID:

Yu-Huan Luo

https://orcid.org/0000-0002-6334-6151

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Journal of Zhejiang University SCIENCE B 2019 Vol.20 No.12 P.972-982

http://doi.org/10.1631/jzus.B1900477


Effects of calcium-binding sites in the S2–S3 loop on human and Nematostella vectensis TRPM2 channel gating processes


Author(s):  Yu-Huan Luo, Xia-Fei Yu, Cheng Ma, Fan Yang, Wei Yang

Affiliation(s):  Department of Biophysics, Institute of Neuroscience, NHC and CAMS Key Laboratory of Medical Neurobiology, School of Medicine, Zhejiang University, Hangzhou 310058, China; more

Corresponding email(s):   yangwei@zju.edu.cn

Key Words:  TRPM2, Calcium-binding site, S2–, S3 loop, Channel activation, Channel inactivation


Yu-Huan Luo, Xia-Fei Yu, Cheng Ma, Fan Yang, Wei Yang. Effects of calcium-binding sites in the S2–S3 loop on human and Nematostella vectensis TRPM2 channel gating processes[J]. Journal of Zhejiang University Science B, 2019, 20(12): 972-982.

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author="Yu-Huan Luo, Xia-Fei Yu, Cheng Ma, Fan Yang, Wei Yang",
journal="Journal of Zhejiang University Science B",
volume="20",
number="12",
pages="972-982",
year="2019",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1900477"
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%T Effects of calcium-binding sites in the S2–S3 loop on human and Nematostella vectensis TRPM2 channel gating processes
%A Yu-Huan Luo
%A Xia-Fei Yu
%A Cheng Ma
%A Fan Yang
%A Wei Yang
%J Journal of Zhejiang University SCIENCE B
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%P 972-982
%@ 1673-1581
%D 2019
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1900477

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T1 - Effects of calcium-binding sites in the S2–S3 loop on human and Nematostella vectensis TRPM2 channel gating processes
A1 - Yu-Huan Luo
A1 - Xia-Fei Yu
A1 - Cheng Ma
A1 - Fan Yang
A1 - Wei Yang
J0 - Journal of Zhejiang University Science B
VL - 20
IS - 12
SP - 972
EP - 982
%@ 1673-1581
Y1 - 2019
PB - Zhejiang University Press & Springer
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DOI - 10.1631/jzus.B1900477


Abstract: 
As a crucial signaling molecule, calcium plays a critical role in many physiological and pathological processes by regulating ion channel activity. Recently, one study resolved the structure of the transient receptor potential melastatin 2 (TRPM2) channel from Nematostella vectensis (nvTRPM2). This identified a calcium-binding site in the s2–;s3 loop, while its effect on channel gating remains unclear. Here, we investigated the role of this calcium-binding site in both nvTRPM2 and human TRPM2 (hTRPM2) by mutagenesis and patch-clamp recording. Unlike hTRPM2, nvTRPM2 cannot be activated by calcium alone. Moreover, the inactivation rate of nvTRPM2 was decreased as intracellular calcium concentration was increased. In addition, our results showed that the four key residues in the calcium-binding site of s2–;s3 loop have similar effects on the gating processes of nvTRPM2 and hTRPM2. Among them, the mutations at negatively charged residues (glutamate and aspartate) substantially decreased the currents of nvTRPM2 and hTRPM2. This suggests that these sites are essential for calcium-dependent channel gating. For the charge-neutralizing residues (glutamine and asparagine) in the calcium-binding site, our data showed that glutamine mutating to alanine or glutamate did not affect the channel activity, but glutamine mutating to lysine caused loss of function. Asparagine mutating to aspartate still remained functional, while asparagine mutating to alanine or lysine led to little channel activity. These results suggest that the side chain of glutamine has a less contribution to channel gating than does asparagine. However, our data indicated that both glutamine mutating to alanine or glutamate and asparagine mutating to aspartate accelerated the channel inactivation rate, suggesting that the calcium-binding site in the s2–;s3 loop is important for calcium-dependent channel inactivation. Taken together, our results uncovered the effect of four key residues in the s2–;s3 loop of TRPM2 on the TRPM2 gating process.

S2-S3 loop中钙离子结合位点对人类及海葵来源TRPM2通道门控过程的影响研究

目的:揭示S2-S3 loop的钙离子结合位点对M2型瞬时受体电位通道(TRPM2)门控过程的影响.
创新点:首次探究了人类TRPM2通道(hTRPM2)和海葵TRPM2通道(nvTRPM2)S2-S3 loop的钙离子结合位点内四个氨基酸残基不同突变对通道激活及失活过程的影响,明确了钙离子结合位点对通道门控的作用.
方法:运用分子突变和电生理检测的方法,系统探究关键位点不同突变对hTRPM2和nvTRPM2激活及失活过程的影响,并采用生物素化及免疫印迹的方法,检测突变对通道表达上膜的影响.
结论:(1)钙离子不能单独激活nvTRPM2通道;(2)hTRPM2和nvTRPM2的四个关键氨基酸对钙离子依赖的门控调节作用类似;(3)在钙离子结合位点的四个关键氨基酸中,两个电负性氨基酸影响通道激活门控,两个电中性氨基酸影响通道失活门控.

关键词:M2型瞬时受体电位通道(TRPM2);钙离子结合位点;S2-S3 loop;通道激活;通道失活

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

Reference

[1]Autzen HE, Myasnikov AG, Campbell MG, et al., 2018. Structure of the human TRPM4 ion channel in a lipid nanodisc. Science, 359(6372):228-232.

[2]Csanády L, Törőcsik B, 2009. Four Ca2+ ions activate TRPM2 channels by binding in deep crevices near the pore but intracellularly of the gate. J Gen Physiol, 133(2):189-203.

[3]Du JY, Xie J, Yue LX, 2009. Intracellular calcium activates TRPM2 and its alternative spliced isoforms. Proc Natl Acad Sci USA, 106(17):7239-7244.

[4]Gao GF, Wang WW, Tadagavadi RK, et al., 2014. TRPM2 mediates ischemic kidney injury and oxidant stress through RAC1. J Clin Invest, 124(11):4989-5001.

[5]Hermosura MC, Cui AM, Go RCV, et al., 2008. Altered functional properties of a TRPM2 variant in Guamanian ALS and PD. Proc Natl Acad Sci USA, 105(46):18029-18034.

[6]Huang S, Turlova E, Li FY, et al., 2017. Transient receptor potential melastatin 2 channels (TRPM2) mediate neonatal hypoxic-ischemic brain injury in mice. Exp Neurol, 296:32-40.

[7]Huang YH, Winkler PA, Sun WN, et al., 2018. Architecture of the TRPM2 channel and its activation mechanism by ADP-ribose and calcium. Nature, 562(7725):145-149.

[8]Jiang LH, Yang W, Zou J, et al., 2010. TRPM2 channel properties, functions and therapeutic potentials. Expert Opin Ther Targets, 14(9):973-988.

[9]Kheradpezhouh E, Ma LL, Morphett A, et al., 2014. TRPM2 channels mediate acetaminophen-induced liver damage. Proc Natl Acad Sci USA, 111(8):3176-3181.

[10]Kühn F, Kühn C, Lückhoff A, 2015. Functional characterisation of a TRPM2 orthologue from the sea anemone Nematostella vectensis in human cells. Sci Rep, 5:8032.

[11]Kühn F, Kühn C, Lückhoff A, 2017. Different principles of ADP-ribose-mediated activation and opposite roles of the NUDT9 homology domain in the TRPM2 orthologs of man and sea anemone. Front Physiol, 8:879.

[12]Lange I, Penner R, Fleig A, et al., 2008. Synergistic regulation of endogenous TRPM2 channels by adenine dinucleotides in primary human neutrophils. Cell Calcium, 44(6):604-615.

[13]Li X, Jiang LH, 2018. Multiple molecular mechanisms form a positive feedback loop driving amyloid β42 peptide-induced neurotoxicity via activation of the TRPM2 channel in hippocampal neurons. Cell Death Dis, 9(2):195.

[14]Luo YH, Yu XF, Ma C, et al., 2018. Identification of a novel EF-Loop in the N-terminus of TRPM2 channel involved in calcium sensitivity. Front Pharmacol, 9:581.

[15]Miller BA, Hoffman NE, Merali S, et al., 2014. TRPM2 channels protect against cardiac ischemia-reperfusion injury: role of mitochondria. J Biol Chem, 289(11):7615-7629.

[16]Nagamine K, Kudoh J, Minoshima S, et al., 1998. Molecular cloning of a novel putative Ca2+ channel protein (TRPC7) highly expressed in brain. Genomics, 54(1):124-131.

[17]Perraud AL, Fleig A, Dunn CA, et al., 2001. ADP-ribose gating of the calcium-permeable LTRPC2 channel revealed by Nudix motif homology. Nature, 411(6837):595-599.

[18]Tóth B, Csanády L, 2010. Identification of direct and indirect effectors of the transient receptor potential melastatin 2 (TRPM2) cation channel. J Biol Chem, 285(39):30091-30102.

[19]Wang LF, Fu TM, Zhou YM, et al., 2018. Structures and gating mechanism of human TRPM2. Science, 362(6421):eaav4809.

[20]Yang W, Zou J, Xia R, et al., 2010. State-dependent inhibition of TRPM2 channel by acidic pH. J Biol Chem, 285(40):30411-30418.

[21]Yang W, Manna PT, Zou J, et al., 2011. Zinc inactivates melastatin transient receptor potential 2 channels via the outer pore. J Biol Chem, 286(27):23789-23798.

[22]Yonezawa R, Yamamoto S, Takenaka M, et al., 2016. TRPM2 channels in alveolar epithelial cells mediate bleomycin-induced lung inflammation. Free Radic Biol Med, 90: 101-113.

[23]Yu PL, Xue XW, Zhang JM, et al., 2017. Identification of the ADPR binding pocket in the NUDT9 homology domain of TRPM2. J Gen Physiol, 149(2):219-235.

[24]Yu WY, Jiang LH, Zheng Y, et al., 2014. Inactivation of TRPM2 channels by extracellular divalent copper. PLoS ONE, 9(11):e112071.

[25]Zhang Z, Tóth B, Szollosi A, et al., 2018. Structure of a TRPM2 channel in complex with Ca2+ explains unique gating regulation. Elife, 7:e36409.

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