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CLC number: R459.9

On-line Access: 2020-08-04

Received: 2019-11-04

Revision Accepted: 2020-02-19

Crosschecked: 2020-07-20

Cited: 0

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Citations:  Bibtex RefMan EndNote GB/T7714


Xue-jun Sun


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Journal of Zhejiang University SCIENCE B 2020 Vol.21 No.8 P.593-602


Therapeutic effect of methane and its mechanism in disease treatment

Author(s):  Zhou-heng Ye, Ke Ning, Bradley P. Ander, Xue-jun Sun

Affiliation(s):  Department of Aviation and Diving Medicine, the Sixth Medical Center, General Hospital of People’s Liberation Army, Beijing 100048, China; more

Corresponding email(s):   sunxjk@126.com

Key Words:  Methane treatment, Ischemia and reperfusion injury, Inflammation, Methanogenesis

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Zhou-heng Ye, Ke Ning, Bradley P. Ander, Xue-jun Sun. Therapeutic effect of methane and its mechanism in disease treatment[J]. Journal of Zhejiang University Science B, 2020, 21(8): 593-602.

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T1 - Therapeutic effect of methane and its mechanism in disease treatment
A1 - Zhou-heng Ye
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A1 - Bradley P. Ander
A1 - Xue-jun Sun
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DOI - 10.1631/jzus.B1900629

Methane is the simplest hydrocarbon, consisting of one carbon atom and four hydrogen atoms. It is abundant in marsh gas, livestock rumination, and combustible ice. Little is known about the use of methane in human disease treatment. Current research indicates that methane is useful for treating several diseases including ischemia and reperfusion injury, and inflammatory diseases. The mechanisms underlying the protective effects of methane appear primarily to involve anti-oxidation, anti-inflammation, and anti-apoptosis. In this review, we describe the beneficial effects of methane on different diseases, summarize possible mechanisms by which methane may act in these conditions, and discuss the purpose of methane production in hypoxic conditions. Then we propose several promising directions for the future research.



Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


[1]Althoff F, Benzing K, Comba P, et al., 2014. Abiotic methanogenesis from organosulphur compounds under ambient conditions. Nat Commun, 5:4205.

[2]Basson AR, Lam M, Cominelli F, 2017. Complementary and alternative medicine strategies for therapeutic gut microbiota modulation in inflammatory bowel disease and their next-generation approaches. Gastroenterol Clin North Am, 46(4):689-729.

[3]Boros M, Keppler F, 2019. Methane production and bioactivity-A link to oxido-reductive stress. Front Physiol, 10:1244.

[4]Boros M, Ghyczy M, Érces D, et al., 2012. The anti-inflammatory effects of methane. Crit Care Med, 40(4):1269-1278.

[5]Boros M, Tuboly E, Mészáros A, et al., 2015. The role of methane in mammalian physiology—is it a gasotransmitter? J Breath Res, 9(1):014001.

[6]Carlisle SM, Burchart PA, McCauley C, et al., 2005. Biokinetics of inhaled radioactive methane in rats: a pilot study. Appl Radiat Isotopes, 62(6):847-860.

[7]Chen OY, Ye ZH, Cao ZY, et al., 2016. Methane attenuates myocardial ischemia injury in rats through anti-oxidative, anti-apoptotic and anti-inflammatory actions. Free Radic Biol Med, 90:1-11.

[8]Cheng X, Zhang L, Xie NC, et al., 2017. Association between small-intestinal bacterial overgrowth and deep vein thrombosis in patients with spinal cord injuries. J Thromb Haemost, 15(2):304-311.

[9]Didychuk C, Burchart PA, Carlisle SM, et al., 2014. Retention and excretion of inhaled 3H and 14C radiolabeled methane in rats. Health Phys, 107(1):18-35.

[10]Fan DF, Hu HJ, Sun Q, et al., 2016. Neuroprotective effects of exogenous methane in a rat model of acute carbon monoxide poisoning. Brain Res, 1633:62-72.

[11]Ghyczy M, Boros M, 2001. Electrophilic methyl groups present in the diet ameliorate pathological states induced by reductive and oxidative stress: a hypothesis. Br J Nutr, 85(4):409-414.

[12]Ghyczy M, Torday C, Boros M, 2003. Simultaneous generation of methane, carbon dioxide, and carbon monoxide from choline and ascorbic acid: a defensive mechanism against reductive stress? FASEB J, 17(9):1124-1126.

[13]Ghyczy M, Torday C, Kaszaki J, et al., 2008. Hypoxia-induced generation of methane in mitochondria and eukaryotic cells—an alternative approach to methanogenesis. Cell Physiol Biochem, 21(1-3):251-258.

[14]He R, Wang LP, Zhu JL, et al., 2016. Methane-rich saline protects against concanavalin A-induced autoimmune hepatitis in mice through anti-inflammatory and anti-oxidative pathways. Biochem Biophys Res Commun, 470(1):22-28.

[15]Jia YF, Li ZY, Feng Y, et al., 2018. Methane-rich saline ameliorates sepsis-induced acute kidney injury through anti-inflammation, antioxidative, and antiapoptosis effects by regulating endoplasmic reticulum stress. Oxid Med Cell Longev, 2018:4756846.

[16]Kawamura T, Wakabayashi N, Shigemura N, et al., 2013. Hydrogen gas reduces hyperoxic lung injury via the Nrf2 pathway in vivo. Am J Physiol Lung Cell Mol Physiol, 304(10):L646-L656.

[17]Koskinen K, Pausan MR, Perras AK, et al., 2017. First insights into the diverse human archaeome: specific detection of archaea in the gastrointestinal tract, lung, and nose and on skin. mBio, 8(6):e00824-17.

[18]Li ZY, Jia YF, Feng Y, et al., 2019. Methane alleviates sepsis-induced injury by inhibiting pyroptosis and apoptosis: in vivo and in vitro experiments. Aging, 11(4):1226-1239.


[20]Liu L, Sun QL, Wang RB, et al., 2016. Methane attenuates retinal ischemia/reperfusion injury via anti-oxidative and anti-apoptotic pathways. Brain Res, 1646:327-333.

[21]Lyu Z, Shao NN, Akinyemi T, et al., 2018. Methanogenesis. Curr Biol, 28(13):R727-R732.

[22]Meng Y, Jiang ZY, Li N, et al., 2018. Protective effects of methane-rich saline on renal ischemic-reperfusion injury in a mouse model. Med Sci Monit, 24:7794-7801.


[24]Polag D, Keppler F, 2018. Long-term monitoring of breath methane. Sci Total Environ, 624:69-77.

[25]Poles MZ, Bódi N, Bagyánszki M, et al., 2018. Reduction of nitrosative stress by methane: neuroprotection through xanthine oxidoreductase inhibition in a rat model of mesenteric ischemia-reperfusion. Free Radic Biol Med, 120:160-169.

[26]Rezaie A, Buresi M, Lembo A, et al., 2017. Hydrogen and methane-based breath testing in gastrointestinal disorders: The North American Consensus. Am J Gastroenterol, 112(5):775-784.

[27]Sogodogo E, Drancourt M, Grine G, 2019. Methanogens as emerging pathogens in anaerobic abscesses. Eur J Clin Microbiol Infect Dis, 38(5):811-818.

[28]Sun AJ, Wang WH, Ye XJ, et al., 2017. Protective effects of methane-rich saline on rats with lipopolysaccharide-induced acute lung injury. Oxid Med Cell Longev, 2017:7430193.

[29]Tuboly E, Szabó A, Garab D, et al., 2013. Methane biogenesis during sodium azide-induced chemical hypoxia in rats. Am J Physiol Cell Physiol, 304(2):C207-C214.

[30]van de Pol JA, van Best N, Mbakwa CA, et al., 2017. Gut colonization by methanogenic archaea is associated with organic dairy consumption in children. Front Microbiol, 8:355.

[31]Wang GH, Xu B, Shi FY, et al., 2019. Protective effect of methane-rich saline on acetic acid-induced ulcerative colitis via blocking the TLR4/NF-κB/MAPK pathway and promoting IL-10/JAK1/STAT3-mediated anti-inflammatory response. Oxid Med Cell Longev, 2019:7850324.

[32]Wang LP, Yao Y, He R, et al., 2017. Methane ameliorates spinal cord ischemia-reperfusion injury in rats: antioxidant, anti-inflammatory and anti-apoptotic activity mediated by Nrf2 activation. Free Radic Biol Med, 103:69-86.

[33]Wu JC, Wang RB, Ye ZH, et al., 2015. Protective effects of methane-rich saline on diabetic retinopathy via anti-inflammation in a streptozotocin-induced diabetic rat model. Biochem Biophys Res Commun, 466:155-161.

[34]Xie Q, Fei MM, Fa ZZ, et al., 2017. Methane-rich saline alleviates cerulein-induced acute pancreatitis by inhibiting inflammatory response, oxidative stress and pancreatic apoptosis in mice. Int Immunopharmacol, 51:17-24.

[35]Yao Y, Wang LP, Jin PP, et al., 2017. Methane alleviates carbon tetrachloride induced liver injury in mice: anti-inflammatory action demonstrated by increased PI3K/ Akt/GSK-3β-mediated IL-10 expression. J Mol Histol, 48(4):301-310.

[36]Ye ZH, Chen OY, Zhang RJ, et al., 2015. Methane attenuates hepatic ischemia/reperfusion injury in rats through antiapoptotic, anti-inflammatory, and antioxidative actions. Shock, 44(2):181-187.

[37]Zhang D, Li N, Wang YY, et al., 2019. Methane ameliorates post-operative cognitive dysfunction by inhibiting microglia NF-κB/MAPKs pathway and promoting IL-10 expression in aged mice. Int immunopharmacol, 71:52-60.

[38]Zhang N, Lu HT, Zhang RJ, et al., 2019. Protective effects of methane-rich saline on mice with allergic asthma by inhibiting inflammatory response, oxidative stress and apoptosis. J Zhejiang Univ-Sci B (Biomed & Biotechnol), 20(10):828-837.

[39]Zhang X, Li N, Shao H, et al., 2016. Methane limit LPS-induced NF-κB/MAPKs signal in macrophages and suppress immune response in mice by enhancing PI3K/AKT/ GSK-3β-mediated IL-10 expression. Sci Rep, 6:29359.

[40]Zhou SZ, Zhou YL, Ji F, et al., 2018. Analgesic effect of methane rich saline in a rat model of chronic inflammatory pain. Neurochem Res, 43(4):869-877.

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