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Abstract: Proteins are major functional units that are tightly connected to form complex and dynamic networks. These networks enable cells and organisms to operate properly and respond efficiently to environmental cues. Over the past decades, many biochemical methods have been developed to search for protein-binding partners in order to understand how protein networks are constructed and connected. At the same time, rapid development in proteomics and mass spectrometry (MS) techniques makes it possible to identify interacting proteins and build comprehensive protein‒protein interaction networks. The resulting interactomes and networks have proven informative in the investigation of biological functions, such as in the field of DNA damage repair. In recent years, a number of proteins involved in DNA damage response and DNA repair pathways have been uncovered with MS-based protein‒protein interaction studies. As the technologies for enriching associated proteins and MS become more sophisticated, the studies of protein‒protein interactions are entering a new era. In this review, we summarize the strategies and recent developments for exploring protein‒protein interaction. In addition, we discuss the application of these tools in the investigation of protein‒protein interaction networks involved in DNA damage response and DNA repair.

基于质谱技术的蛋白质互作研究方法进展及其在DNA损伤修复中的应用

摘要：蛋白质作为主要的生物功能分子往往通过相互作用形成复合体和动态的互作网络来行使其功能。这些互作网络使得细胞和器官能够正常行使功能，并有效地应对外界环境的刺激。在过去的几十年里，为了研究蛋白质相互作用网络，研究人员开发了许多生物化学方法来寻找目标蛋白质的相互作用蛋白。与此同时，蛋白质组和质谱技术的快速发展也使得鉴定相互作用蛋白并构建相关蛋白质互作网络成为了可能。利用这些蛋白质鉴定技术，蛋白质互作网络取得了很多重要进展，包括在DNA损伤修复研究领域。在近些年，通过蛋白质互作研究，大量的DNA损伤应答和修复相关蛋白被发现。而随着蛋白质富集和质谱技术的进一步发展，蛋白质互作研究进入了一个新的阶段。在这篇综述里，我们总结了蛋白质互作研究策略的最新进展，以及这些技术在DNA损伤应答及其修复研究中的应用。

关键词：蛋白质互作；相互作用组；蛋白质组；质谱；DNA损伤修复；DNA损伤应答

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