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On-line Access: 2023-12-08
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Fibrillarin promotes homologous recombination repair by facilitating the recruitment of recombinase RAD51 to DNA damage sites
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Yanhua MU, Jinhua HAN, Mingjie WU, Zongfang LI, Ke DU, Yameng WEI, Mengjie WU, Jun HUANG. Fibrillarin promotes homologous recombination repair by facilitating the recruitment of recombinase RAD51 to DNA damage sites[J]. Journal of Zhejiang University Science B, 2023, 24(12): 1165-1173.
@article{title="Fibrillarin promotes homologous recombination repair by facilitating the recruitment of recombinase RAD51 to DNA damage sites",
author="Yanhua MU, Jinhua HAN, Mingjie WU, Zongfang LI, Ke DU, Yameng WEI, Mengjie WU, Jun HUANG",
journal="Journal of Zhejiang University Science B",
volume="24",
number="12",
pages="1165-1173",
year="2023",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B2300518"
}
%0 Journal Article
%T Fibrillarin promotes homologous recombination repair by facilitating the recruitment of recombinase RAD51 to DNA damage sites
%A Yanhua MU
%A Jinhua HAN
%A Mingjie WU
%A Zongfang LI
%A Ke DU
%A Yameng WEI
%A Mengjie WU
%A Jun HUANG
%J Journal of Zhejiang University SCIENCE B
%V 24
%N 12
%P 1165-1173
%@ 1673-1581
%D 2023
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B2300518
TY - JOUR
T1 - Fibrillarin promotes homologous recombination repair by facilitating the recruitment of recombinase RAD51 to DNA damage sites
A1 - Yanhua MU
A1 - Jinhua HAN
A1 - Mingjie WU
A1 - Zongfang LI
A1 - Ke DU
A1 - Yameng WEI
A1 - Mengjie WU
A1 - Jun HUANG
J0 - Journal of Zhejiang University Science B
VL - 24
IS - 12
SP - 1165
EP - 1173
%@ 1673-1581
Y1 - 2023
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B2300518
Abstract: Eukaryotic organisms constantly face a wide range of internal and external factors that cause damage to their DNA. Failure to accurately and efficiently repair these DNA lesions can result in genomic instability and the development of tumors (Canela et al., 2017). Among the various forms of DNA damage, DNA double-strand breaks (DSBs) are particularly harmful. Two major pathways, non-homologous end joining (NHEJ) and homologous recombination (HR), are primarily responsible for repairing DSBs (Katsuki et al., 2020; Li and Yuan, 2021; Zhang and Gong, 2021; Xiang et al., 2023). NHEJ is an error-prone repair mechanism that simply joins the broken ends together (Blunt et al., 1995; Hartley et al., 1995). In contrast, HR is a precise repair process. It involves multiple proteins in eukaryotic cells, with the RAD51 recombinase being the key player, which is analogous to bacterial recombinase A (RecA) (Shinohara et al., 1992). The central event in HR is the formation of RAD51-single-stranded DNA (ssDNA) nucleoprotein filaments that facilitate homology search and DNA strand invasion, ultimately leading to the initiation of repair synthesis (Miné et al., 2007; Hilario et al., 2009; Ma et al., 2017).
[1]BluntT, FinnieNJ, TaccioliGE, et al., 1995. Defective DNA-dependent protein kinase activity is linked to V(D)J recombination and DNA repair defects associated with the murine scid mutation. Cell, 80(5):813-823.
[2]CanelaA, MamanY, JungS, et al., 2017. Genome organization drives chromosome fragility. Cell, 170(3):507-521.e18.
[3]HanahanD, 2022. Hallmarks of cancer: new dimensions. Cancer Discov, 12(1):31-46.
[4]HartleyKO, GellD, SmithGCM, et al., 1995. DNA-dependent protein kinase catalytic subunit: a relative of phosphatidylinositol 3-kinase and the ataxia telangiectasia gene product. Cell, 82(5):849-856.
[5]HilarioJ, AmitaniI, BaskinRJ, et al., 2009. Direct imaging of human Rad51 nucleoprotein dynamics on individual DNA molecules. Proc Natl Acad Sci USA, 106(2):361-368.
[6]KatsukiY, JeggoPA, UchiharaY, et al., 2020. DNA double-strand break end resection: a critical relay point for determining the pathway of repair and signaling. Genome Instab Dis, 1(4):155-171.
[7]LiYH, YuanJ, 2021. Role of deubiquitinating enzymes in DNA double-strand break repair. J Zhejiang Univ-Sci B (Biomed & Biotechnol), 22(1):63-72.
[8]MaCJ, GibbB, KwonY, et al., 2017. Protein dynamics of human RPA and RAD51 on ssDNA during assembly and disassembly of the RAD51 filament. Nucleic Acids Res, 45(2):749-761.
[9]MarcelV, GhayadSE, BelinS, et al., 2013. p53 acts as a safeguard of translational control by regulating fibrillarin and rRNA methylation in cancer. Cancer Cell, 24(3):318-330.
[10]MinéJ, DisseauL, TakahashiM, et al., 2007. Real-time measurements of the nucleation, growth and dissociation of single Rad51-DNA nucleoprotein filaments. Nucleic Acids Res, 35(21):7171-7187.
[11]NewtonK, PetfalskiE, TollerveyD, et al., 2003. Fibrillarin is essential for early development and required for accumulation of an intron-encoded small nucleolar RNA in the mouse. Mol Cell Biol, 23(23):8519-8527.
[12]OmerAD, ZiescheS, EbhardtH, et al., 2002. In vitro reconstitution and activity of a C/D box methylation guide ribonucleoprotein complex. Proc Natl Acad Sci USA, 99(8):5289-5294.
[13]SchimmangT, TollerveyD, KernH, et al., 1989. A yeast nucleolar protein related to mammalian fibrillarin is associated with small nucleolar RNA and is essential for viability. EMBO J, 8(13):4015-4024.
[14]ShinoharaA, OgawaH, OgawaT, 1992. Rad51 protein involved in repair and recombination in S. cerevisiae is a RecA-like protein. Cell, 69(3):457-470.
[15]SnaarS, WiesmeijerK, JochemsenAG, et al., 2000. Mutational analysis of fibrillarin and its mobility in living human cells. J Cell Biol, 151(3):653-662.
[16]TessarzP, Santos-RosaH, RobsonSC, et al., 2014. Glutamine methylation in histone H2A is an RNA-polymerase-I-dedicated modification. Nature, 505(7484):564-568.
[17]TikuV, JainC, RazY, et al., 2017. Small nucleoli are a cellular hallmark of longevity. Nat Commun, 8:16083.
[18]TollerveyD, LehtonenH, JansenR, et al., 1993. Temperature-sensitive mutations demonstrate roles for yeast fibrillarin in pre-rRNA processing, pre-rRNA methylation, and ribosome assembly. Cell, 72(3):443-457.
[19]XiangZY, LiuH, HuY, 2023. DNA damage repair and cancer immunotherapy. Genome Instab Dis, 4(4):210-226.
[20]YiYH, MaTH, LeeLW, et al., 2015. A genetic cascade of let-7-ncl-1-fib-1 modulates nucleolar size and rRNA pool in Caenorhabditis elegans. PLoS Genet, 11(10):e1005580.
[21]YoshikawaH, KomatsuW, HayanoT, et al., 2011. Splicing factor 2-associated protein p32 participates in ribosome biogenesis by regulating the binding of Nop52 and fibrillarin to preribosome particles. Mol Cell Proteomics, 10(8):M110.006148.
[22]ZhangF, GongZH, 2021. Regulation of DNA double-strand break repair pathway choice: a new focus on 53BP1. J Zhejiang Univ-Sci B (Biomed & Biotechnol), 22(1):38-46.
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