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On-line Access: 2021-09-10

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 ORCID:

Xianyong LAN

https://orcid.org/0000-0002-6104-2904

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Journal of Zhejiang University SCIENCE B 2021 Vol.22 No.9 P.757-766

http://doi.org/10.1631/jzus.B2100079


Insertion/deletion variants within the IGF2BP2 gene identified in reported genome-wide selective sweep analysis reveal a correlation with goat litter size


Author(s):  Dongyun XIN, Yangyang BAI, Yi BI, Libang HE, Yuxin KANG, Chuanying PAN, Haijing ZHU, Hong CHEN, Lei QU, Xianyong LAN

Affiliation(s):  Lab of Animal Genome and Gene Function, College of Animal Science and Technology, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Northwest A&F University, Yangling 712100, China; more

Corresponding email(s):   lanxianyong79@126.com

Key Words:  Goat, Insulin-like growth factor 2 mRNA-binding protein 2 (IGF2BP2), Litter size, Indel, Marker-assisted selection (MAS)


Dongyun XIN, Yangyang BAI, Yi BI, Libang HE, Yuxin KANG, Chuanying PAN, Haijing ZHU, Hong CHEN, Lei QU, Xianyong LAN. Insertion/deletion variants within the IGF2BP2 gene identified in reported genome-wide selective sweep analysis reveal a correlation with goat litter size[J]. Journal of Zhejiang University Science B, 2021, 22(9): 757-766.

@article{title="Insertion/deletion variants within the IGF2BP2 gene identified in reported genome-wide selective sweep analysis reveal a correlation with goat litter size",
author="Dongyun XIN, Yangyang BAI, Yi BI, Libang HE, Yuxin KANG, Chuanying PAN, Haijing ZHU, Hong CHEN, Lei QU, Xianyong LAN",
journal="Journal of Zhejiang University Science B",
volume="22",
number="9",
pages="757-766",
year="2021",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B2100079"
}

%0 Journal Article
%T Insertion/deletion variants within the IGF2BP2 gene identified in reported genome-wide selective sweep analysis reveal a correlation with goat litter size
%A Dongyun XIN
%A Yangyang BAI
%A Yi BI
%A Libang HE
%A Yuxin KANG
%A Chuanying PAN
%A Haijing ZHU
%A Hong CHEN
%A Lei QU
%A Xianyong LAN
%J Journal of Zhejiang University SCIENCE B
%V 22
%N 9
%P 757-766
%@ 1673-1581
%D 2021
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B2100079

TY - JOUR
T1 - Insertion/deletion variants within the IGF2BP2 gene identified in reported genome-wide selective sweep analysis reveal a correlation with goat litter size
A1 - Dongyun XIN
A1 - Yangyang BAI
A1 - Yi BI
A1 - Libang HE
A1 - Yuxin KANG
A1 - Chuanying PAN
A1 - Haijing ZHU
A1 - Hong CHEN
A1 - Lei QU
A1 - Xianyong LAN
J0 - Journal of Zhejiang University Science B
VL - 22
IS - 9
SP - 757
EP - 766
%@ 1673-1581
Y1 - 2021
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B2100079


Abstract: 
Insulin-like growth factor 2 mRNA-binding protein 2 (IGF2BP2, also called IMP2) plays an essential role in the development and maturation of germ cells and embryos and is a candidate gene for goat litter size, based on a previous genome-wide selective sweep analysis. In this study, the mRNA expression level of IGF2BP2 was found to be significantly higher in a single-lamb group than in a multi-lamb group. Insertions/deletions (indels) within the goat IGF2BP2 gene, including P4-Ins-13bp and P5-Del-12bp, were verified in 918 Shaanbei White Cashmere (SBWC) female goats. The minor allelic frequencies (MAFs) of P4-Ins-13bp and P5-Del-12bp loci were 0.349 and 0.295, respectively. Analysis using the Chi-square (χ2) test showed that the genotype (χ2=14.479, P=0.006) distribution of P4-Ins-13bp was significantly different between the single-lamb and multi-lamb groups. Correlation analysis demonstrated that P4-Ins-13bp was significantly associated with goat litter size (P=0.022), and individual goats with the homozygous deletion/deletion (DD) genotype produced more litters than other goats. Therefore, considered as a potential molecular marker significantly related to lambing traits, the P4-Ins-13bp mutation of the goat IGF2BP2 gene can be used in goat breeding with practical molecular marker-assisted selection (MAS) to optimize female reproduction and improve economic efficiency in the goat industry.

已报道的全基因组选择性消除分析中发现IGF2BP2基因的插入/缺失变异与山羊产羔数相关

目的:验证IGF2BP2插入/缺失与陕北白绒山羊产羔数之间的关系,并选择有利的等位基因。
创新点:首次筛选、验证山羊IGF2BP2基因的InDel位点,确定其与山羊产羔性状的相关性及作用,为山羊经济性状改良及新品种培育提供理论依据。
方法:采用实时荧光定量聚合酶链式反应(qRT-PCR)方法分析IGF2BP2在单羔组和多羔组中的表达水平;通过PCR扩增-琼脂糖凝胶电泳在918只陕北白绒母山羊中鉴定IGF2BP2基因P4-Ins-13bp和P5-Del-12bp缺失/插入位点基因型,并进行测序以验证突变;利用SHEsis平台分析两个位点的连锁不平衡;采用方差分析(ANOVA)和χ2检验分析山羊IGF2BP2基因变异与产羔数的关系;并利用在线数据库预测转录因子(TF)和突变区域序列的结合。
结论:本研究发现,IGF2BP2的mRNA表达水平在单羔组明显高于多羔组。在陕北白绒母山羊中验证了IGF2BP2基因内的插入/缺失(indels),包括P4-Ins-13bp和P5-Del-12bp。χ2检验表明,P4-Ins-13bp基因型(χ2=14.479,P=0.006)在单羔和多羔组间分布差异显著;相关分析表明,P4-Ins-13bp与山羊产仔数显着相关(P=0.022),具有缺失/缺失(DD)基因型的山羊个体产仔数高于其他山羊。因此,IGF2BP2基因的P4-Ins-13bp突变可作为一种潜在的与繁殖性状显著相关的分子标记,以优化母羊繁殖力,提高山羊产业的经济效益。

关键词:山羊;IGF2BP2;产仔数;插入/缺失;分子标记辅助选择

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

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