CLC number:
On-line Access: 2021-09-10
Received: 2021-01-26
Revision Accepted: 2021-03-31
Crosschecked: 0000-00-00
Cited: 0
Clicked: 4293
Dongyun XIN, Yangyang BAI, Yi BI, Libang HE, Yuxin KANG, Chuanying PAN, Haijing ZHU, Hong CHEN, Lei QU, Xianyong LAN. Insertion/deletion variants within the IGF2BP2 gene identified in reported genome-wide selective sweep analysis reveal a correlation with goat litter size[J]. Journal of Zhejiang University Science B, 2021, 22(9): 757-766.
@article{title="Insertion/deletion variants within the IGF2BP2 gene identified in reported genome-wide selective sweep analysis reveal a correlation with goat litter size",
author="Dongyun XIN, Yangyang BAI, Yi BI, Libang HE, Yuxin KANG, Chuanying PAN, Haijing ZHU, Hong CHEN, Lei QU, Xianyong LAN",
journal="Journal of Zhejiang University Science B",
volume="22",
number="9",
pages="757-766",
year="2021",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B2100079"
}
%0 Journal Article
%T Insertion/deletion variants within the IGF2BP2 gene identified in reported genome-wide selective sweep analysis reveal a correlation with goat litter size
%A Dongyun XIN
%A Yangyang BAI
%A Yi BI
%A Libang HE
%A Yuxin KANG
%A Chuanying PAN
%A Haijing ZHU
%A Hong CHEN
%A Lei QU
%A Xianyong LAN
%J Journal of Zhejiang University SCIENCE B
%V 22
%N 9
%P 757-766
%@ 1673-1581
%D 2021
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B2100079
TY - JOUR
T1 - Insertion/deletion variants within the IGF2BP2 gene identified in reported genome-wide selective sweep analysis reveal a correlation with goat litter size
A1 - Dongyun XIN
A1 - Yangyang BAI
A1 - Yi BI
A1 - Libang HE
A1 - Yuxin KANG
A1 - Chuanying PAN
A1 - Haijing ZHU
A1 - Hong CHEN
A1 - Lei QU
A1 - Xianyong LAN
J0 - Journal of Zhejiang University Science B
VL - 22
IS - 9
SP - 757
EP - 766
%@ 1673-1581
Y1 - 2021
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B2100079
Abstract: Insulin-like growth factor 2 mRNA-binding protein 2 (IGF2BP2, also called IMP2) plays an essential role in the development and maturation of germ cells and embryos and is a candidate gene for goat litter size, based on a previous genome-wide selective sweep analysis. In this study, the mRNA expression level of IGF2BP2 was found to be significantly higher in a single-lamb group than in a multi-lamb group. Insertions/deletions (indels) within the goat IGF2BP2 gene, including P4-Ins-13bp and P5-Del-12bp, were verified in 918 Shaanbei White Cashmere (SBWC) female goats. The minor allelic frequencies (MAFs) of P4-Ins-13bp and P5-Del-12bp loci were 0.349 and 0.295, respectively. Analysis using the Chi-square (χ2) test showed that the genotype (χ2=14.479, P=0.006) distribution of P4-Ins-13bp was significantly different between the single-lamb and multi-lamb groups. Correlation analysis demonstrated that P4-Ins-13bp was significantly associated with goat litter size (P=0.022), and individual goats with the homozygous deletion/deletion (DD) genotype produced more litters than other goats. Therefore, considered as a potential molecular marker significantly related to lambing traits, the P4-Ins-13bp mutation of the goat IGF2BP2 gene can be used in goat breeding with practical molecular marker-assisted selection (MAS) to optimize female reproduction and improve economic efficiency in the goat industry.
[1]AbdoliR, ZamaniP, MirhoseiniSZ, et al., 2019. Genetic parameters and trends for litter size in Markhoz goats. Rev Colomb Cienc Pec, 32(1):58-63.
[2]AlexR, RameshaKP, SinghU, et al., 2018. Promoter variants of OAS1 gene are associated with reproductive performance and incidence of normal calving in cattle. Theriogenology, 108:255-261.
[3]BiY, FengB, WangZ, et al., 2020. Myostatin (MSTN) gene indel variation and its associations with body traits in Shaanbei White Cashmere goat. Animals (Basel), 10(1):168.
[4]BiswasJ, PatelVL, BhaskarV, et al., 2019. The structural basis for RNA selectivity by the IMP family of RNA-binding proteins. Nat Commun, 10:4440.
[5]BrantsJR, AyoubiTAY, ChadaK, et al., 2004. Differential regulation of the insulin-like growth factor II mRNA-binding protein genes by architectural transcription factor HMGA2. FEBS Lett, 569(1-3):277-283.
[6]BüngerL, RonaldML, RothschildMF, et al., 2005. Relationships between quantitative and reproductive fitness traits in animals. Philos Trans R Soc Lond B Biol Sci, 360(1459):1489-1502.
[7]ChenSC, QiuH, LiuC, et al., 2018. Relationship between IGF2BP2 and IGFBP3 polymorphisms and susceptibility to non-small-cell lung cancer: a case-control study in Eastern Chinese Han population. Cancer Manag Res, 10:2965-2975.
[8]ChennathukuzhiV, SteinJM, AbelT, et al., 2003. Mice deficient for testis-brain RNA-binding protein exhibit a coordinate loss of TRAX, reduced fertility, altered gene expression in the brain, and behavioral changes. Mol Cell Biol, 23(18):6419-6434.
[9]CollardBCY, MackillDJ, 2008. Marker-assisted selection: an approach for precision plant breeding in the twenty-first century. Philos Trans Roy Soc B, 363(1491):557-572.
[10]DaiN, RapleyJ, AngelM, et al., 2011. mTOR phosphorylates IMP2 to promote IGF2 mRNA translation by internal ribosomal entry. Genes Dev, 25(11):1159-1172.
[11]E GX, ZhaoYJ, HuangYF, 2019. Selection signatures of litter size in Dazu black goats based on a whole genome sequencing mixed pools strategy. Mol Biol Rep, 46(5):5517-5523.
[12]HammerNA, HansenTVO, ByskovAG, et al., 2005. Expression of IGF-II mRNA-binding proteins (IMPs) in gonads and testicular cancer. Reproduction, 130(2):203-212.
[13]HouS, QuDJ, LiY, et al., 2019. XAB2 depletion induces intron retention in POLR2A to impair global transcription and promote cellular senescence. Nucl Acids Res, 47(15):8239-8254.
[14]HuiYQ, ZhangYH, WangK, et al., 2020. Goat DNMT3B: an indel mutation detection, association analysis with litter size and mRNA expression in gonads. Theriogenology, 147:108-115.
[15]JiaWC, WuXF, LiXC, et al., 2015. Novel genetic variants associated with mRNA expression of signal transducer and activator of transcription 3 (STAT3) gene significantly affected goat growth traits. Small Ruminant Res, 129:25-36.
[16]KangZH, JiangEH, WangK, et al., 2019a. Goat membrane associated ring-CH-type finger 1 (MARCH1) mRNA expression and association with litter size. Theriogenology, 128:8-16.
[17]KangZH, ZhangSH, HeLB, et al., 2019b. A 14-bp functional deletion within the CMTM2 gene is significantly associated with litter size in goat. Theriogenology, 139:49-57.
[18]KinK, NnamaniMC, LynchVJ, et al., 2015. Cell-type phylogenetics and the origin of endometrial stromal cells. Cell Rep, 10(8):1398-1409.
[19]KumarS, StecherG, TamuraK, 2016. MEGA7: Molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol, 33(7):1870-1874.
[20]LiHX, XuHW, AkhatayevaH, et al., 2021. Novel indel variations of the sheep FecB gene and their effects on litter size. Gene, 767:145176.
[21]LiW, LiuD, ChangW, et al., 2014. Role of IGF2BP3 in trophoblast cell invasion and migration. Cell Death Dis, 5:e1025.
[22]LiuGH, ZhuTN, CuiYJ, et al., 2015. Correlation between IGF2BP2 gene polymorphism and the risk of breast cancer in Chinese Han women. Biomed Pharmacother, 69:297-300.
[23]LiuHB, MuhammadT, GuoYS, et al., 2019. RNA-binding protein IGF2BP2/IMP2 is a critical maternal activator in early zygotic genome activation. Adv Sci (Weinh), 6(15):1900295.
[24]LiuHF, LiHX, MaoC, et al., 2021. Insights into genetic variants within sheep IGF2BP1 and their association with litter size. Small Ruminant Res, 98:106350.
[25]LuCD, MillerBA, 2019. Current status, challenges and prospects for dairy goat production in the Americas. Asian-Australas J Anim Sci, 32(8):1244-1255.
[26]MuQC, WangLJ, YuFB, et al., 2015. Imp2 regulates GBM progression by activating IGF2/PI3K/Akt pathway. Cancer Biol Ther, 16(4):623-633.
[27]MuñozM, FernándezAI, ÓviloC, et al., 2010. Non-additive effects of RBP4, ESR1 and IGF2 polymorphisms on litter size at different parities in a Chinese-European porcine line. Genet Sel Evol, 42:23.
[28]PanXY, LiuSJ, LiFD, et al., 2014. Molecular characterization, expression profiles of the ovine FSHR gene and its association with litter size. Mol Biol Rep, 41(12):7749-7754.
[29]RaoP, WangH, FangHH, et al., 2016. Association between IGF2BP2 polymorphisms and type 2 diabetes mellitus: a case-control study and meta-analysis. Int J Environ Res Public Health, 13(6):574.
[30]RuggiuM, SpeedR, TaggartM, et al., 1997. The mouse Dazla gene encodes a cytoplasmic protein essential for gametogenesis. Nature, 389(6646):73-77.
[31]TangQ, ZhangY, YangY, et al., 2021. The KMT2A gene: mRNA differential expression in the ovary and a novel 13-nt nucleotide sequence variant associated with litter size in cashmere goats. Domest Anim Endocrinol, 74:106538.
[32]WangJJ, ZhaoM, XiaoJP, et al., 2016. E-Cadherin, CD44v6, and insulin-like growth factor-II mRNA-Binding protein 3 expressions in different stages of hydatidiform moles. J Biochem Mol Toxicol, 30(9):455-461.
[33]WangJY, LanJ, ZhaoJG, et al., 2012. Molecular characterization, polymorphism and association of porcine SPATA19 gene. Mol Biol Rep, 39(10):9741-9746.
[34]WangXY, YangQ, WangK, et al., 2017. A novel 12-bp indel polymorphism within the GDF9 gene is significantly associated with litter size and growth traits in goats. Anim Genet, 48(6):735-736.
[35]WangXY, YangQ, WangK, et al., 2019. Two strongly linked single nucleotide polymorphisms (Q320P and V397I) in GDF9 gene are associated with litter size in cashmere goats. Theriogenology, 125:115-121.
[36]WangZ, ZhangX, JiangE, et al., 2020. InDels within caprine IGF2BP1 intron 2 and the 3'-untranslated regions are associated with goat growth traits. Anim Genet, 51(1):117-121.
[37]WuH, PanY, ZhangQF, et al., 2019. Insertion/deletion (InDel) variations in sheep PLAG1 gene locating in growth-related major QTL are associated with adult body weight and morphometric traits. Small Ruminant Res, 178:63-69.
[38]YinH, DuX, LiQQ, et al., 2019. Variants in BMP7 and BMP15 3'-UTRs associated with reproductive traits in a Large White pig population. Animals (Basel), 9(11):905.
[39]YongY, HeL, 2005. SHEsis, a powerful software platform for analyses of linkage disequilibrium, haplotype construction, and genetic association at polymorphism loci. Cell Res, 15(2):97-98.
[40]ZhaoY, MaYS, FangY, et al., 2012. IGF2BP2 genetic variation and type 2 diabetes: a global meta-analysis. DNA Cell Biol, 31(5):713-720.
[41]ZhengWM, GraferCM, KimJ, et al., 2015. Gonadotropin-releasing hormone and gonadal steroids regulate transcription factor mRNA expression in primary pituitary and immortalized gonadotrope cells. Reprod Sci, 22(3):285-299.
Open peer comments: Debate/Discuss/Question/Opinion
<1>