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Received: 2021-12-26

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Wenqing ZHANG


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Journal of Zhejiang University SCIENCE B 2022 Vol.23 No.6 P.515-527


Functional characterization of piggyBac-like elements from Nilaparvata lugens (Stål) (Hemiptera: Delphacidae)

Author(s):  Jun LYU, Qin SU, Jinhui LIU, Lin CHEN, Jiawei SUN, Wenqing ZHANG

Affiliation(s):  State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, China

Corresponding email(s):   lsszwq@mail.sysu.edu.cn

Key Words:  Nilaparvata lugens, piggyBac-like elements, Transposon, NlPLE25

Jun LYU, Qin SU, Jinhui LIU, Lin CHEN, Jiawei SUN, Wenqing ZHANG. Functional characterization of piggyBac-like elements from Nilaparvata lugens (Stål) (Hemiptera: Delphacidae)[J]. Journal of Zhejiang University Science B, 2022, 23(6): 515-527.

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author="Jun LYU, Qin SU, Jinhui LIU, Lin CHEN, Jiawei SUN, Wenqing ZHANG",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Functional characterization of piggyBac-like elements from Nilaparvata lugens (Stål) (Hemiptera: Delphacidae)
%A Jun LYU
%A Qin SU
%A Jinhui LIU
%A Jiawei SUN
%A Wenqing ZHANG
%J Journal of Zhejiang University SCIENCE B
%V 23
%N 6
%P 515-527
%@ 1673-1581
%D 2022
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B2101090

T1 - Functional characterization of piggyBac-like elements from Nilaparvata lugens (Stål) (Hemiptera: Delphacidae)
A1 - Jun LYU
A1 - Qin SU
A1 - Jinhui LIU
A1 - Lin CHEN
A1 - Jiawei SUN
A1 - Wenqing ZHANG
J0 - Journal of Zhejiang University Science B
VL - 23
IS - 6
SP - 515
EP - 527
%@ 1673-1581
Y1 - 2022
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B2101090

PiggyBac is a transposable DNA element originally discovered in the cabbage looper moth (Trichoplusia ni). The T. ni piggyBac transposon can introduce exogenous fragments into a genome, constructing a transgenic organism. Nevertheless, the comprehensive analysis of endogenous piggyBac-like elements (PLEs) is important before using piggyBac, because they may influence the genetic stability of transgenic lines. Herein, we conducted a genome-wide analysis of PLEs in the brown planthopper (BPH) Nilaparvata lugens (Stål) (Hemiptera: Delphacidae), and identified a total of 28 PLE sequences. All N. lugens piggyBac-like elements (NlPLEs) were present as multiple copies in the genome of BPH. Among the identified NlPLEs, NlPLE25 had the highest copy number and it was distributed on five chromosomes. The full length of NlPLE25 consisted of terminal inverted repeats and sub-terminal inverted repeats at both terminals, as well as a single open reading frame transposase encoding 546 amino acids. Furthermore, NlPLE25 transposase caused precise excision and transposition in cultured insect cells and also restored the original TTAA target sequence after excision. A cross-recognition between the NlPLE25 transposon and the piggyBac transposon was also revealed in this study. These findings provide useful information for the construction of transgenic insect lines.




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