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On-line Access: 2023-01-10

Received: 2022-08-30

Revision Accepted: 2022-10-26

Crosschecked: 2023-01-16

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Citations:  Bibtex RefMan EndNote GB/T7714

 ORCID:

Xun WANG

https://orcid.org/0000-0003-2234-144X

Zhihui WANG

Zhihui WANG, https://orcid.org/0000-0003-2234-144X

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Journal of Zhejiang University SCIENCE B 2023 Vol.24 No.1 P.94-100

http://doi.org/10.1631/jzus.B2200431


Carotenoid components and their biosynthesis in a bud mutant of Shiranui mandarin (Citrus reticulata Blanco) with citrine flavedo


Author(s):  Xun WANG, Jinqiu HUANG, Zongyan YIN, Ke XU, Dong JIANG, Lijin LIN, Xiaoai ZHANG, Zhihui WANG

Affiliation(s):  Institute of Pomology and Olericulture, Sichuan Agricultural University, Chengdu 611130, China; more

Corresponding email(s):   wangzhihui318@126.com

Key Words:  Citrine Shiranui, Flavedo pigment, HPLC, Carotenoid proportion, Gene transcript level


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Xun WANG, Jinqiu HUANG, Zongyan YIN, Ke XU, Dong JIANG, Lijin LIN, Xiaoai ZHANG, Zhihui WANG. Carotenoid components and their biosynthesis in a bud mutant of Shiranui mandarin (Citrus reticulata Blanco) with citrine flavedo[J]. Journal of Zhejiang University Science B, 2023, 24(1): 94-100.

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author="Xun WANG, Jinqiu HUANG, Zongyan YIN, Ke XU, Dong JIANG, Lijin LIN, Xiaoai ZHANG, Zhihui WANG",
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pages="94-100",
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publisher="Zhejiang University Press & Springer",
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Abstract: 
Carotenoids are secondary metabolite responsible for colored pigments in plants and microbes (Li et al., 2022). They are a class of C40 tetraterpenoids consisting of eight isoprenoid units, and can be classified into carotenes and xanthophylls on the basis of their functional groups (Saini et al., 2015). Carotenes can be linear (phytoene, phytofluene, and ζ‍-carotene) or branched (β‍-carotene and α‍-carotene). Xanthophylls comprise β,β‍-xanthophylls (β‍-cryptoxanthin, zeaxanthin, violaxanthins, and neoxanthin) and β,ϵ‍-xanthophylls (α-cryptoxanthin, α-carotene, and lutein). Citrus fruits are complex sources of carotenoids, which are the principal pigments responsible for the typical orange color of most types (Chen, 2020). The difference in total carotenoid content and the diversity of carotenoid isomer proportion also accounts for other colors of citrus fruits, such as yellow, red, and pink (Chen, 2020).

"不知火"黄色果皮芽变品种中类胡罗卜素组分及生物合成

王迅1,黄金秋2,尹宗艳2,许轲3,江东4,林立金1,张小艾2,汪志辉2
1四川农业大学果蔬研究所,中国成都市,611130
2四川农业大学园艺学院,中国成都市,611130
3四川省园艺作物技术推广总站,中国成都市,610041
4西南大学柑桔研究所,中国重庆市,400712
摘要:宽皮柑橘"柠檬不知火"是"不知火"的自然芽变品种,其果皮呈类似柠檬果皮的亮黄色,明显区别于"不知火"果皮的橘红色。本研究通过分析上述两种柑橘果皮中类胡罗卜素的含量和组分,及与其合成代谢相关基因的表达量,解析两种柑橘果皮颜色的差异机制。测定结果显示,"不知火"中总类胡罗卜素含量显著高于芽变品种"柠檬不知火",推测这是造成两种果皮颜色差异的主要原因。芽变品种中呈现橙色色素的组分β-隐黄质占比低于"不知火";而与绿-黄色调有关的叶黄素和新黄质这两种类胡罗卜素在芽变品种中的占比高于"不知火"。"不知火"中PSY基因的高表达可能为其类胡罗卜素合成提供了充足的底物,从而促使其果皮中较高水平的类胡罗卜素合成;HYb基因在所有基因中表达量最高,推测与其参与催化类胡罗卜素代谢途径中多个反应有关;而ZEP基因的低表达量可能与类胡罗卜素代谢途径下游产物的反馈调节有关。

关键词:柠檬不知火;果皮色泽;高效液相色谱(HPLC);类胡罗卜素组分;基因表达

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

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