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Journal of Zhejiang University SCIENCE B 2023 Vol.24 No.5 P.430-441


Rice bran oil supplementation protects swine weanlings against diarrhea and lipopolysaccharide challenge

Author(s):  Juncheng HUANG, Wenxia QIN, Baoyang XU, Haihui SUN, Fanghua JING, Yunzheng XU, Jianan ZHAO, Yuwen CHEN, Libao MA, Xianghua YAN

Affiliation(s):  State Key Laboratory of Agricultural Microbiology, Hubei Hongshan Laboratory, Frontiers Science Center for Animal Breeding and Sustainable Production, College of Animal Sciences and Technology, Huazhong Agricultural University, Wuhan 430070, China; more

Corresponding email(s):   malibao@mail.hzau.edu.cn, xhyan@mail.hzau.edu.cn

Key Words:  Rice bran oil, Gut microbiota, Weaned piglets, Oxidative stress, Lipopolysaccharide

Juncheng HUANG, Wenxia QIN, Baoyang XU, Haihui SUN, Fanghua JING, Yunzheng XU, Jianan ZHAO, Yuwen CHEN, Libao MA, Xianghua YAN. Rice bran oil supplementation protects swine weanlings against diarrhea and lipopolysaccharide challenge[J]. Journal of Zhejiang University Science B, 2023, 24(5): 430-441.

@article{title="Rice bran oil supplementation protects swine weanlings against diarrhea and lipopolysaccharide challenge",
author="Juncheng HUANG, Wenxia QIN, Baoyang XU, Haihui SUN, Fanghua JING, Yunzheng XU, Jianan ZHAO, Yuwen CHEN, Libao MA, Xianghua YAN",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Rice bran oil supplementation protects swine weanlings against diarrhea and lipopolysaccharide challenge
%A Juncheng HUANG
%A Wenxia QIN
%A Baoyang XU
%A Haihui SUN
%A Fanghua JING
%A Yunzheng XU
%A Jianan ZHAO
%A Yuwen CHEN
%A Libao MA
%A Xianghua YAN
%J Journal of Zhejiang University SCIENCE B
%V 24
%N 5
%P 430-441
%@ 1673-1581
%D 2023
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B2200565

T1 - Rice bran oil supplementation protects swine weanlings against diarrhea and lipopolysaccharide challenge
A1 - Juncheng HUANG
A1 - Wenxia QIN
A1 - Baoyang XU
A1 - Haihui SUN
A1 - Fanghua JING
A1 - Yunzheng XU
A1 - Jianan ZHAO
A1 - Yuwen CHEN
A1 - Libao MA
A1 - Xianghua YAN
J0 - Journal of Zhejiang University Science B
VL - 24
IS - 5
SP - 430
EP - 441
%@ 1673-1581
Y1 - 2023
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B2200565

Early weaned piglets suffer from oxidative stress and enteral infection, which usually results in gut microbial dysbiosis, serve diarrhea, and even death. rice bran oil (RBO), a polyphenol-enriched by-product of rice processing, has been shown to have antioxidant and anti-inflammatory properties both in vivo and in vitro. Here, we ascertained the proper RBO supplementation level, and subsequently determined its effects on lipopolysaccharide (LPS)-induced intestinal dysfunction in weaned piglets. A total of 168 piglets were randomly allocated into four groups of seven replicates (42 piglets each group, (21±1) d of age, body weight (7.60±0.04) kg, and half males and half females) and were given basal diet (Ctrl) or basal diet supplemented with 0.01% (mass fraction) RBO (RBO1), 0.02% RBO (RBO2), or 0.03% RBO (RBO3) for 21 d. Then, seven piglets from the Ctrl and the RBO were treated with LPS (100 μg/kg body weight (BW)) as LPS group and RBO+LPS group, respectively. Meanwhile, seven piglets from the Ctrl were treated with the saline vehicle (Ctrl group). Four hours later, all treated piglets were sacrificed for taking samples of plasma, jejunum tissues, and feces. The results showed that 0.02% was the optimal dose of dietary RBO supplementation based on diarrhea, average daily gain, and average daily feed intake indices in early weaning piglets. Furthermore, RBO protected piglets against LPS-induced jejunal epithelium damage, which was indicated by the increases in villus height, villus height/crypt depth ratio, and Claudin-1 levels, as well as a decreased level of jejunal epithelium apoptosis. RBO also improved the antioxidant ability of LPS-challenged piglets, which was indicated by the elevated concentrations of catalase and superoxide dismutase, and increased total antioxidant capacity, as well as the decreased concentrations of diamine oxidase and malondialdehyde in plasma. Meanwhile, RBO improved the immune function of LPS-challenged weaned piglets, which was indicated by elevated immunoglobulin A (IgA), IgM, β‍‍-defensin-1, and lysozyme levels in the plasma. In addition, RBO supplementation improved the LPS challenge-induced dysbiosis of gut microbiota. Particularly, the indices of antioxidant capacity, intestinal damage, and immunity were significantly associated with the RBO-regulated gut microbiota. These findings suggested that 0.02% RBO is a suitable dose to protect against LPS-induced intestinal damage, oxidative stress, and jejunal microbiota dysbiosis in early weaned piglets.


黄俊程1,2,3, 秦文峡1,2,3, 徐保阳1,2,3, 孙海辉4, 京芳华4, 徐云政1,2,3, 赵嘉楠1,2,3, 陈昱文1,2,3, 马立保1,2,3, 晏向华1,2,3
1华中农业大学动物科学技术学院, 农业微生物学国家重点实验室, 湖北洪山实验室, 动物育种与健康养殖前沿科学中心, 中国武汉市, 430070
2生猪健康养殖省部共建协同创新中心, 中国武汉市, 430070
3生猪精准饲养与饲料安全技术湖北省工程实验室, 中国武汉市, 430070
4宜春市大海龟生命科学有限公司, 中国宜春市, 336000
摘要:早期断奶仔猪遭受氧化应激和肠道感染,通常会导致肠道微生物失调、腹泻甚至死亡。米糠油(RBO)是一种富含多酚的大米加工副产品,在体内和体外都具有抗氧化和抗炎特性。本研究中,我们确定了早期断奶仔猪日粮中适当的RBO补充水平,随后确定了它对脂多糖(LPS)诱导的断奶仔猪肠道功能障碍的影响。将168头仔猪随机分4组,每组7个重复(42头/组,(21±1)日龄,体重(7.60±0.04) kg,公母各半),分别接受基础日粮(Ctrl)或补充基础日粮含0.01% RBO(RBO1)、0.02% RBO(RBO2)或0.03% RBO(RBO3)。饲喂21天后,Ctrl和RBO组的7头仔猪经LPS(100 µg/kg BW)处理(分别为LPS组和RBO+LPS组)。同时将Ctrl组的7头仔猪用载体盐水处理作为对照(Ctrl组)。四小时后,处死所有处理组仔猪并采集血浆、空肠组织和粪便。分别检测血浆中抗氧化和免疫指标,评估空肠组织形态和屏障功能以及通过16S rDNA测序分析肠道微生物组成、功能及多样性。结果表明,根据早期断奶仔猪的腹泻、平均日增重和平均日采食量指标,0.02%是日粮中添加RBO的最佳剂量。此外,RBO可以缓解LPS诱导的仔猪空肠上皮损伤,表现为绒毛高度、绒毛高度/隐窝深度比和Claudin-1水平的增加,以及空肠上皮细胞凋亡的改善。RBO还提高了LPS应激仔猪的抗氧化能力,表现为血浆中过氧化氢酶和超氧化物歧化酶浓度升高,总抗氧化能力提升,以及二胺氧化酶和丙二醛浓度降低。 同时,RBO提高了LPS应激的断奶仔猪的免疫功能,表现为血浆中IgA、IgM、β-防御素-1和溶菌酶升高。此外,补充RBO还改善了LPS应激引起的肠道菌群失调。相关性分析结果发现,仔猪抗氧化能力、肠道损伤和免疫力指标分别与RBO调节的肠道微生物群显着相关。综上所述,0.02% RBO是缓解LPS诱导的早期断奶仔猪肠道损伤、氧化应激和空肠微生物群失调的适当剂量。


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[1]AlbenbergL, EsipovaTV, JudgeCP, et al., 2014. Correlation between intraluminal oxygen gradient and radial partitioning of intestinal microbiota. Gastroenterology, 147(5):1055-1063.e8.

[2]AllenHK, LevineUY, LooftT, et al., 2013. Treatment, promotion, commotion: antibiotic alternatives in food-producing animals. Trends Microbiol, 21(3):114-119.

[3]BaoT, ZhangM, ZhouYQ, et al., 2021. Phenolic profile of jujube fruit subjected to gut microbiota fermentation and its antioxidant potential against ethyl carbamate-induced oxidative damage. J Zhejiang Univ-Sci B (Biomed & Biotechnol), 22(5):397-409.

[4]BhandariSK, XuB, NyachotiCM, et al., 2008. Evaluation of alternatives to antibiotics using an Escherichia coli K88+ model of piglet diarrhea: effects on gut microbial ecology. J Anim Sci, 86(4):836-847.

[5]BolerDD, Fernández-DueñasDM, KutzlerLW, et al., 2012. Effects of oxidized corn oil and a synthetic antioxidant blend on performance, oxidative status of tissues, and fresh meat quality in finishing barrows. J Anim Sci, 90(13):5159-5169.

[6]BolyenE, RideoutJR, DillonMR, et al., 2019. Reproducible, interactive, scalable and extensible microbiome data science using QIIME 2. Nat Biotechnol, 37(8):852-857.

[7]CampbellJM, CrenshawJD, PoloJ, 2013. The biological stress of early weaned piglets. J Anim Sci Biotechnol, 4:19.

[8]ChenCC, WangZB, LiJZ, et al., 2019. Dietary vitamin E affects small intestinal histomorphology, digestive enzyme activity, and the expression of nutrient transporters by inhibiting proliferation of intestinal epithelial cells within jejunum in weaned piglets. J Anim Sci, 97(3):1212-1221.

[9]DouS, Gadonna-WidehemP, RomeV, et al., 2017. Characterisation of early-life fecal microbiota in susceptible and healthy pigs to post-weaning diarrhoea. PLoS ONE, 12(1):e0169851.

[10]DouglasGM, MaffeiVJ, ZaneveldJR, et al., 2020. PICRUSt2 for prediction of metagenome functions. Nat Biotechnol, 38(6):685-688.

[11]FragouS, FegerosK, XylouriE, et al., 2004. Effect of vitamin E supplementation on various functional properties of macrophages and neutrophils obtained from weaned piglets. J Vet Med Ser A, 51(4):178-183.

[12]FreseSA, ParkerK, CalvertCC, et al., 2015. Diet shapes the gut microbiome of pigs during nursing and weaning. Microbiome, 3:28.

[13]GavrieliY, ShermanY, Ben-SassonSA, 1992. Identification of programmed cell death in situ via specific labeling of nuclear DNA fragmentation. J Cell Biol, 119(3):493-501.

[14]GreinerT, BäckhedF, 2011. Effects of the gut microbiota on obesity and glucose homeostasis. Trends Endocrinol Metab, 22(4):117-123.

[15]GresseR, Chaucheyras-DurandF, FleuryMA, et al., 2017. Gut microbiota dysbiosis in postweaning piglets: understanding the keys to health. Trends Microbiol, 25(10):851-873.

[16]HuRZ, WuSS, LiBZ, et al., 2022. Dietary ferulic acid and vanillic acid on inflammation, gut barrier function and growth performance in lipopolysaccharide-challenged piglets. Anim Nutr, 8:144-152.

[17]HuangCY, FanZJ, HanDD, et al., 2021. Pyrroloquinoline quinone regulates the redox status in vitro and in vivo of weaned pigs via the Nrf2/HO-1 pathway. J Anim Sci Biotechnol, 12:77.

[18]KaakoushNO, 2015. Insights into the role of Erysipelotrichaceae in the human host. Front Cell Infect Microbiol, 5:84.

[19]KushwahaR, 2018. Pharmacognosy of rice bran oil—a review. Int J Green Pharm, 12(4):S784-S789.

[20]LiuKY, NakatsuCH, Jones-HallY, et al., 2021. Vitamin E alpha- and gamma-tocopherol mitigate colitis, protect intestinal barrier function and modulate the gut microbiota in mice. Free Radical Biol Med, 163:180-189.

[21]LuT, HarperAF, ZhaoJ, et al., 2014. Supplementing antioxidants to pigs fed diets high in oxidants: I. Effects on growth performance, liver function, and oxidative status. J Anim Sci, 92(12):5455-5463.

[22]MocchegianiE, CostarelliL, GiacconiR, et al., 2014. Vitamin E-gene interactions in aging and inflammatory age-related diseases: implications for treatment. A systematic review. Ageing Res Rev, 14:81-101.

[23](National Research Council)NRC, 2012. Nutrient Requirements of Swine, 11th Rev. Ed. National Academics Press, Washington, DC.

[24]PosuwanJ, PrangthipP, LeardkamolkarnV, et al., 2013. Long-term supplementation of high pigmented rice bran oil (Oryza sativa L.) on amelioration of oxidative stress and histological changes in streptozotocin-induced diabetic rats fed a high fat diet; Riceberry bran oil. Food Chem, 138(1):501-508.

[25]PratesJAM, FreireJPB, de AlmeidaAM, et al., 2021. Influence of dietary supplementation with an amino acid mixture on inflammatory markers, immune status and serum proteome in LPS-challenged weaned piglets. Animals, 11(4):1143.

[26]PuniaS, KumarM, SirohaAK, et al., 2021. Rice bran oil: emerging trends in extraction, health benefit, and its industrial application. Rice Sci, 28(3):217-232.

[27]QiuYQ, YangJ, WangL, et al., 2021. Dietary resveratrol attenuation of intestinal inflammation and oxidative damage is linked to the alteration of gut microbiota and butyrate in piglets challenged with deoxynivalenol. J Anim Sci Biotechnol, 12:71.

[28]RenW, YuB, YuJ, et al., 2022. Lower abundance of Bacteroides and metabolic dysfunction are highly associated with the post-weaning diarrhea in piglets. Sci China Life Sci, 65(10):2062-2075.

[29]SchneiderCA, RasbandWS, EliceiriKW, 2012. NIH image to imageJ: 25 years of image analysis. Nat Methods, 9(7):671-675.

[30]SegataN, IzardJ, WaldronL, et al., 2011. Metagenomic biomarker discovery and explanation. Genome Biol, 12(6):R60.

[31]ShangQS, ShanXD, CaiC, et al., 2016. Dietary fucoidan modulates the gut microbiota in mice by increasing the abundance of Lactobacillus and Ruminococcaceae. Food Funct, 7(7):3224-3232.

[32]Silva-GuillenYV, ArellanoC, BoydRD, et al., 2020. Growth performance, oxidative stress and immune status of newly weaned pigs fed peroxidized lipids with or without supplemental vitamin E or polyphenols. J Anim Sci Biotechnol, 11:22.

[33]SinghPK, WiseSY, DuceyEJ, et al., 2011. α-Tocopherol succinate protects mice against radiation-induced gastrointestinal injury. Radiat Res, 177(2):133-145.

[34]StarkeIC, PieperR, NeumannK, et al., 2014. The impact of high dietary zinc oxide on the development of the intestinal microbiota in weaned piglets. FEMS Microbiol Ecol, 87(2):416-427.

[35]SunX, CuiYL, SuYY, et al., 2021. Dietary fiber ameliorates lipopolysaccharide-induced intestinal barrier function damage in piglets by modulation of intestinal microbiome. mSystems, 6(2):e01374-20.

[36]WangXF, TsaiT, DengFL, et al., 2019. Longitudinal investigation of the swine gut microbiome from birth to market reveals stage and growth performance associated bacteria. Microbiome, 7:109.

[37]WangY, ZhangRM, LiJY, et al., 2017. Comprehensive resistome analysis reveals the prevalence of NDM and MCR-1 in Chinese poultry production. Nat Microbiol, 2(4):16260.

[38]WinterSE, WinterMG, XavierMN, et al., 2013. Host-derived nitrate boosts growth of E. coli in the inflamed gut. Science, 339(6120):708-711.

[39]XuBY, QinWX, XuYZ, et al., 2021a. Dietary quercetin supplementation attenuates diarrhea and intestinal damage by regulating gut microbiota in weanling piglets. Oxid Med Cell Longev, 2021:6221012.

[40]XuBY, YanYQ, YinBQ, et al., 2021b. Dietary glycyl-glutamine supplementation ameliorates intestinal integrity, inflammatory response, and oxidative status in association with the gut microbiota in LPS-challenged piglets. Food Funct, 12(8):3539-3551.

[41]XuX, WangXY, WuHT, et al., 2018. Glycine relieves intestinal injury by maintaining mTOR signaling and suppressing AMPK, TLR4, and NOD signaling in weaned piglets after lipopolysaccharide challenge. Int J Mol Sci, 19(7):1980.

[42]XuX, HuaHW, WangLM, et al., 2020. Holly polyphenols alleviate intestinal inflammation and alter microbiota composition in lipopolysaccharide-challenged pigs. Br J Nutr, 123(8):881-891.

[43]YanYQ, XuBY, YinBQ, et al., 2020. Modulation of gut microbial community and metabolism by dietary glycyl-glutamine supplementation may favor weaning transition in piglets. Front Microbiol, 10:3125.

[44]YardeniT, TanesCE, BittingerK, et al., 2019. Host mitochondria influence gut microbiome diversity: a role for ROS. Sci Signal, 12(588):eaaw3159.

[45]YiGF, CarrollJA, AlleeGL, et al., 2005. Effect of glutamine and spray-dried plasma on growth performance, small intestinal morphology, and immune responses of Escherichia coli K88+-challenged weaned pigs. J Anim Sci, 83(3):634-643.

[46]ZengMY, InoharaN, NuñezG, 2017. Mechanisms of inflammation-driven bacterial dysbiosis in the gut. Mucosal Immunol, 10(1):18-26.

[47]ZhangYC, MuTQ, JiaH, et al., 2022. Protective effects of glycine against lipopolysaccharide-induced intestinal apoptosis and inflammation. Amino Acids, 54(3):353-364.

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