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Chaoyue WEN, Hong ZHANG, Qiuping GUO, Yehui DUAN, Sisi CHEN, Mengmeng HAN, Fengna LI, Mingliang JIN, Yizhen WANG. Engineered Bacillus subtilis alleviates intestinal oxidative injury through Nrf2-Keap1 pathway in ETEC K88-infected piglet[J]. Journal of Zhejiang University Science B, 1998, -1(-1): .
@article{title="Engineered Bacillus subtilis alleviates intestinal oxidative injury through Nrf2-Keap1 pathway in ETEC K88-infected piglet",
author="Chaoyue WEN, Hong ZHANG, Qiuping GUO, Yehui DUAN, Sisi CHEN, Mengmeng HAN, Fengna LI, Mingliang JIN, Yizhen WANG",
journal="Journal of Zhejiang University Science B",
volume="-1",
number="-1",
pages="",
year="1998",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B2200674"
}
%0 Journal Article
%T Engineered Bacillus subtilis alleviates intestinal oxidative injury through Nrf2-Keap1 pathway in ETEC K88-infected piglet
%A Chaoyue WEN
%A Hong ZHANG
%A Qiuping GUO
%A Yehui DUAN
%A Sisi CHEN
%A Mengmeng HAN
%A Fengna LI
%A Mingliang JIN
%A Yizhen WANG
%J Journal of Zhejiang University SCIENCE B
%V -1
%N -1
%P
%@ 1673-1581
%D 1998
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B2200674
TY - JOUR
T1 - Engineered Bacillus subtilis alleviates intestinal oxidative injury through Nrf2-Keap1 pathway in ETEC K88-infected piglet
A1 - Chaoyue WEN
A1 - Hong ZHANG
A1 - Qiuping GUO
A1 - Yehui DUAN
A1 - Sisi CHEN
A1 - Mengmeng HAN
A1 - Fengna LI
A1 - Mingliang JIN
A1 - Yizhen WANG
J0 - Journal of Zhejiang University Science B
VL - -1
IS - -1
SP -
EP -
%@ 1673-1581
Y1 - 1998
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B2200674
Abstract: engineered probiotics can serve as therapeutics based on their ability of produce recombinant immune-stimulating properties. In this study, we built the recombinant Bacillus subtilis WB800 expressing antimicrobial peptide KR32 (WB800-KR32) using genetic engineering methods and investigated its protective effects of nrf2-Keap1 pathway activation in intestinal oxidative disturbance induced by ETEC K88 in weaned piglets. Twenty-eight weaned piglets were randomly distributed into four treatment groups with seven replicates fed with a basal diet. The feed of the control group (CON) was infused with normal sterilized saline; meanwhile, the ETEC, ETEC+WB800, and ETEC+WB800-KR32 groups were orally administered normal sterilized saline, 1010 cfu WB800, and 1010 cfu WB800-KR32, respectively, all infused with enterotoxigenic Escherichia coli (ETEC) at d1-14 and K88 at d15-17. The results showed that pretreatment with WB800-KR32 attenuated ETEC-induced intestinal disturbance, improved the mucosal activity of antioxidant enzyme (catalase, superoxide dismutase (SOD) and glutathione peroxidase (GPx)) and decreased the content of malondialdehyde. More importantly, WB800-KR32 downregulated genes involved in antioxidant defense (GPx and SOD1). Interestingly, WB800-KR32 upregulated the protein expression of Nrf2 and downregulated the protein expression of Keap1 in the ileum. WB800-KR32 markedly changed the richness estimators (Ace and Chao) of gut microbiota and increased the abundance of Eubacterium_rectale_ATCC_33656 in the feces. The results suggested that WB800-KR32 may alleviate ETEC-induced intestinal oxidative injury through the nrf2-Keap1 pathway, providing a new perspective for WB800-KR32 as potential therapeutics to regulate intestinal oxidative disturbance in ETEC K88 infection.
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