CLC number:
On-line Access: 2024-08-27
Received: 2023-10-17
Revision Accepted: 2024-05-08
Crosschecked: 2023-08-08
Cited: 0
Clicked: 980
Citations: Bibtex RefMan EndNote GB/T7714
Fang WU, Chen LU, Wenhao HU, Xin GUO, Jiayue CHEN, Zhidan LUO. Rapid visual detection of Vibrio parahaemolyticus by combining LAMP-CRISPR/Cas12b with heat-labile uracil-DNA glycosylase to eliminate carry-over contamination[J]. Journal of Zhejiang University Science B, 2023, 24(8): 749-754.
@article{title="Rapid visual detection of Vibrio parahaemolyticus by combining LAMP-CRISPR/Cas12b with heat-labile uracil-DNA glycosylase to eliminate carry-over contamination",
author="Fang WU, Chen LU, Wenhao HU, Xin GUO, Jiayue CHEN, Zhidan LUO",
journal="Journal of Zhejiang University Science B",
volume="24",
number="8",
pages="749-754",
year="2023",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B2200705"
}
%0 Journal Article
%T Rapid visual detection of Vibrio parahaemolyticus by combining LAMP-CRISPR/Cas12b with heat-labile uracil-DNA glycosylase to eliminate carry-over contamination
%A Fang WU
%A Chen LU
%A Wenhao HU
%A Xin GUO
%A Jiayue CHEN
%A Zhidan LUO
%J Journal of Zhejiang University SCIENCE B
%V 24
%N 8
%P 749-754
%@ 1673-1581
%D 2023
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B2200705
TY - JOUR
T1 - Rapid visual detection of Vibrio parahaemolyticus by combining LAMP-CRISPR/Cas12b with heat-labile uracil-DNA glycosylase to eliminate carry-over contamination
A1 - Fang WU
A1 - Chen LU
A1 - Wenhao HU
A1 - Xin GUO
A1 - Jiayue CHEN
A1 - Zhidan LUO
J0 - Journal of Zhejiang University Science B
VL - 24
IS - 8
SP - 749
EP - 754
%@ 1673-1581
Y1 - 2023
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B2200705
Abstract: vibrio parahaemolyticus is a major pathogen frequently found in seafood. Rapid and accurate detection of this pathogen is important for the control of bacterial foodborne diseases and to ensure food safety. In this study, we established a one-pot system that combines uracil-DNA glycosylase (UDG), loop-mediated isothermal amplification (LAMP), and clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 12b (Cas12b) for detecting V. parahaemolyticus in seafood. This detection system can effectively perform identification using a single tube and avoid the risk of carry-over contamination.
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