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Journal of Zhejiang University SCIENCE B 2024 Vol.25 No.1 P.65-82

http://doi.org/10.1631/jzus.B23d0004


Bioceramic scaffolds with two-step internal/external modification of copper-containing polydopamine enhance antibacterial and alveolar bone regeneration capability


Author(s):  Xiaojian JIANG, Lihong LEI, Weilian SUN, Yingming WEI, Jiayin HAN, Shuaiqi ZHONG, Xianyan YANG, Zhongru GOU, Lili CHEN

Affiliation(s):  Department of Oral Medicine, the Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310009, China; more

Corresponding email(s):   chenlili_1030@zju.edu.cn, zhrgou@zju.edu.cn

Key Words:  Copper-containing polydopamine, Modification, Antibacterial property, Bone regeneration, Angiogenesis, Bioceramic scaffold


Xiaojian JIANG, Lihong LEI, Weilian SUN, Yingming WEI, Jiayin HAN, Shuaiqi ZHONG, Xianyan YANG, Zhongru GOU, Lili CHEN. Bioceramic scaffolds with two-step internal/external modification of copper-containing polydopamine enhance antibacterial and alveolar bone regeneration capability[J]. Journal of Zhejiang University Science B, 2024, 25(1): 65-82.

@article{title="Bioceramic scaffolds with two-step internal/external modification of copper-containing polydopamine enhance antibacterial and alveolar bone regeneration capability",
author="Xiaojian JIANG, Lihong LEI, Weilian SUN, Yingming WEI, Jiayin HAN, Shuaiqi ZHONG, Xianyan YANG, Zhongru GOU, Lili CHEN",
journal="Journal of Zhejiang University Science B",
volume="25",
number="1",
pages="65-82",
year="2024",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B23d0004"
}

%0 Journal Article
%T Bioceramic scaffolds with two-step internal/external modification of copper-containing polydopamine enhance antibacterial and alveolar bone regeneration capability
%A Xiaojian JIANG
%A Lihong LEI
%A Weilian SUN
%A Yingming WEI
%A Jiayin HAN
%A Shuaiqi ZHONG
%A Xianyan YANG
%A Zhongru GOU
%A Lili CHEN
%J Journal of Zhejiang University SCIENCE B
%V 25
%N 1
%P 65-82
%@ 1673-1581
%D 2024
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B23d0004

TY - JOUR
T1 - Bioceramic scaffolds with two-step internal/external modification of copper-containing polydopamine enhance antibacterial and alveolar bone regeneration capability
A1 - Xiaojian JIANG
A1 - Lihong LEI
A1 - Weilian SUN
A1 - Yingming WEI
A1 - Jiayin HAN
A1 - Shuaiqi ZHONG
A1 - Xianyan YANG
A1 - Zhongru GOU
A1 - Lili CHEN
J0 - Journal of Zhejiang University Science B
VL - 25
IS - 1
SP - 65
EP - 82
%@ 1673-1581
Y1 - 2024
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B23d0004


Abstract: 
Magnesium-doped calcium silicate (CS) bioceramic scaffolds have unique advantages in mandibular defect repair; however, they lack antibacterial properties to cope with the complex oral microbiome. Herein, for the first time, the CS scaffold was functionally modified with a novel copper-containing polydopamine (PDA(Cu2+‍)) rapid deposition method, to construct internally modified (*P), externally modified (@PDA), and dually modified (*P@PDA) scaffolds. The morphology, degradation behavior, and mechanical properties of the obtained scaffolds were evaluated in vitro. The results showed that the CS*P@PDA had a unique micro-/nano-structural surface and appreciable mechanical resistance. During the prolonged immersion stage, the release of copper ions from the CS*P@PDA scaffolds was rapid in the early stage and exhibited long-term sustained release. The in vitro evaluation revealed that the release behavior of copper ions ascribed an excellent antibacterial effect to the CS*P@PDA, while the scaffolds retained good cytocompatibility with improved osteogenesis and angiogenesis effects. Finally, the PDA(Cu2+)-modified scaffolds showed effective early bone regeneration in a critical-size rabbit mandibular defect model. Overall, it was indicated that considerable antibacterial property along with the enhancement of alveolar bone regeneration can be imparted to the scaffold by the two-step PDA(Cu2+) modification, and the convenience and wide applicability of this technique make it a promising strategy to avoid bacterial infections on implants.

含铜聚多巴胺内外双修饰法构建抗菌促骨再生生物陶瓷支架

蒋晓健1,雷利红1,孙伟莲1,韦应明1,韩佳吟1,钟帅祺1,杨贤燕2,苟中入2,陈莉丽1
1浙江大学医学院附属第二医院口腔内科,中国杭州市,310009
2浙江加州国际纳米技术研究院生物纳米材料与再生医学研究平台,中国杭州市,310058
摘要:掺镁硅酸钙(CS)生物陶瓷支架在修复下颌骨缺损中具有独特的优势,然而抗菌性能的缺乏使其难以应对复杂的口腔环境。本文首次采用新型含铜聚多巴胺快速沉积方法对CS支架进行功能修饰,分别构建了内修饰支架(CS*P)、外修饰支架(CS@PDA)以及内外双修饰支架(CS*P@PDA)。通过体外对支架表面形貌、降解行为及机械性能进行评价,我们发现双修饰支架具有独特的微纳结构表面和可观的机械强度。在浸泡降解过程中,双修饰支架组的铜离子在早期会快速释放,而后呈现长期的缓释状态。体外抗菌及细胞试验提示,铜离子梯度释放模式在赋予双修饰支架优异抗菌效果的同时,保持了其良好的生物相容性,并且具有促进成骨和成血管的效果。此外,双修饰支架在兔下颌骨临界骨缺损模型中展现了可靠的早期促骨再生效果。总之,通过含铜聚多巴胺内外双修饰法可构建兼具抗菌能力和促骨再生能力的骨再生支架,该修饰方法简便易行且适用性广泛,有望为预防植入物感染提供新思路。

关键词:含铜聚多巴胺;修饰;抗菌性能;骨再生;成血管;生物陶瓷支架

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

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