Abstract: Wounds pose a risk to the skin, our body’s primary defence against infections. The rise of antibiotic resistance has prompted the development of novel therapies. RO-101® is an antimicrobial gel that delivers therapeutic levels of hydrogen peroxide (H2O2), a reactive oxygen species, directly to the wound bed. In this study, electrospinning was used to incorporate RO 101® into a polyvinyl alcohol (PVA) sub-micron fibrous mesh that can act as a delivery agent, achieve a sustained release profile, and provide a barrier against infection. Adequate incorporation of this gel into sub-micron fibres was confirmed via nuclear magnetic resonance spectroscopy. Furthermore, scanning electron microscopy exhibited smooth and uniform meshes with diameters in the 200–500 nm range. PVA/RO-101 electrospun meshes generated H2O2 in concentrations exceeding 1 mM/(g·mL) (1 mM = 1 mmol/L) after 24 h, and the role of sterilisation on H2O2 release was evaluated. PVA/RO-101 meshes exhibited antimicrobial activity against both Gram-positive Staphylococcus aureus (S. aureus) and Gram-negative Pseudomonas aeruginosa (P. aeruginosa) bacteria, achieving viable count reductions of up to 1 log unit CFU/mm2 (CFU: colony-forming units). Moreover, these mesheswerecapableofdisruptingbiofilmformation,evenagainstmultidrug-resistant organisms such as methicillin-resistant S. aureus (MRSA). Furthermore, increasing the RO-101® concentration resulted in higher H2O2 production and an enhanced antimicrobial effect, while fibroblast cell viability and proliferation tests showed a concentration-dependent response with high cytocompatibility at low RO-101® concentrations. This study therefore demon strates the potential of highly absorbent PVA/RO-101 meshes as potential antimicrobial wound dressings.

Similar articles