Full Text:
<213>
CLC number:
On-line Access: 2024-08-27
Received: 2023-10-17
Revision Accepted: 2024-05-08
Crosschecked: 0000-00-00
Cited: 0
Clicked: 358
|
Electrospun Polyvinyl Alcohol Fibres Incorporating an Antimicrobial Gel for Enzymatically Controlled Reactive Oxygen Species Release
| |||||||||||||
Joel Yupanqui Mieles , Cian Vyas, Evangelos Daskalakis , Mohamed Hassan , James Birkett , Abdalla M. Omar , Gavin Humphreys , Carl Diver , Paulo Bartolo. Electrospun Polyvinyl Alcohol Fibres Incorporating an Antimicrobial Gel for Enzymatically Controlled Reactive Oxygen Species Release[J]. Journal of Zhejiang University Science , , (): .
@article{title="Electrospun Polyvinyl Alcohol Fibres Incorporating an
Antimicrobial Gel for Enzymatically Controlled Reactive Oxygen
Species Release",
author="Joel Yupanqui Mieles , Cian Vyas, Evangelos Daskalakis , Mohamed Hassan , James Birkett , Abdalla M. Omar , Gavin Humphreys , Carl Diver , Paulo Bartolo",
journal="Journal of Zhejiang University Science ",
volume="",
number="",
pages="",
year="",
publisher="Zhejiang University Press & Springer",
doi="10.1007/s42242-024-00312-3"
}
%0 Journal Article
%T Electrospun Polyvinyl Alcohol Fibres Incorporating an
Antimicrobial Gel for Enzymatically Controlled Reactive Oxygen
Species Release
%A Joel Yupanqui Mieles
%A Cian Vyas
%A Evangelos Daskalakis
%A Mohamed Hassan
%A James Birkett
%A Abdalla M. Omar
%A Gavin Humphreys
%A Carl Diver
%A Paulo Bartolo
%J Journal of Zhejiang University SCIENCE
%V
%N
%P
%@ 1869-1951
%D
%I Zhejiang University Press & Springer
%DOI 10.1007/s42242-024-00312-3
TY - JOUR
T1 - Electrospun Polyvinyl Alcohol Fibres Incorporating an
Antimicrobial Gel for Enzymatically Controlled Reactive Oxygen
Species Release
A1 - Joel Yupanqui Mieles
A1 - Cian Vyas
A1 - Evangelos Daskalakis
A1 - Mohamed Hassan
A1 - James Birkett
A1 - Abdalla M. Omar
A1 - Gavin Humphreys
A1 - Carl Diver
A1 - Paulo Bartolo
J0 - Journal of Zhejiang University Science
VL -
IS -
SP -
EP -
%@ 1869-1951
Y1 -
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1007/s42242-024-00312-3
Abstract: Wounds pose a risk to the skin, our bodys primary defence against infections. The rise of antibiotic
resistance has prompted the development of novel therapies. RO-101 is an antimicrobial gel that
delivers therapeutic levels of hydrogen peroxide (H2O2), a reactive oxygen species, directly to the
wound bed. In this study, electrospinning was used to incorporate RO-101 into a polyvinyl alcohol
(PVA) submicron fibrous mesh that can act as a delivery agent, achieve a sustained release profile,
and provide a barrier against infection. Adequate incorporation of this gel into submicron fibres was
confirmed via nuclear magnetic resonance spectroscopy. Furthermore, scanning electron microscopy
exhibited smooth and uniform meshes with diameters in the 200500 nm range. PVA/RO-101
electrospun meshes generated H2O2 in concentrations exceeding 1 mM/(mL?g) (1 mM=1 mmol/L)
after 24 h, and the role of sterilisation on H2O2 release was evaluated. PVA/RO-101 meshes exhibited
Preprint of Bio-Design and Manufacturing (unedited)
antimicrobial activity against both Gram-positive Staphylococcus aureus (S. aureus) and Gramnegative Pseudomonas aeruginosa (P. aeruginosa) bacteria, achieving viable count reductions of up
to 1 log unit CFU/mm2
(CFU: colony-forming units). Moreover, these meshes were capable of
disrupting biofilm formation, even against multidrug-resistant organisms such as methicillin-resistant
S. aureus (MRSA). Furthermore, increasing the RO-101 concentration resulted in higher H2O2
production and an enhanced antimicrobial effect, while fibroblast cell viability and proliferation tests
showed a concentration-dependent response with high cytocompatibility at low RO-101
concentrations. This study therefore demonstrates the potential of highly absorbent PVA/RO-101
meshes as potential antimicrobial wound dressings.
Open peer comments: Debate/Discuss/Question/Opinion
<1>