Journal of Zhejiang University

Bio-Design and Manufacturing 2026 Vol.9 No.5 P.1023 - 1039

A dynamic non-invasive monitoring platform for multidimensional functional assessment of Kuanxiong aerosol against cardiac arrhythmias

Author(s): Youmin Ying,Xiaohuan Guo,Kaiqiang Li,Guangyong Ye,Qingqing Lv,Juxing Zheng,Pan Chen,Tao Chen,Ke Hao,Kai Qian,Chengwen He,Zhen Wang
Affiliation(s): 1. College of Pharmaceutical Science, Zhejiang University of Technology, Hangzhou 310014, China more
Corresponding email(s): qiankai@zju.edu.cn, hcw_7_14@163.com, wangzhen@hmc.edu.cn
Key Words: Microelectrode array, Real-time monitoring, Arrhythmia, Kuanxiong aerosol, Cardiomyocytes

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Youmin Ying, Xiaohuan Guo, Kaiqiang Li, Guangyong Ye, Qingqing Lv, Juxing Zheng, Pan Chen, Tao Chen, Ke Hao, Kai Qian, Chengwen He, Zhen Wang. A dynamic non-invasive monitoring platform for multidimensional functional assessment of Kuanxiong aerosol against cardiac arrhythmias[J]. Journal of Zhejiang University Science D, 2026, 9(5): 1023 - 1039.

@article{title="A dynamic non-invasive monitoring platform for multidimensional functional assessment of Kuanxiong aerosol against cardiac arrhythmias",
author="Youmin Ying, Xiaohuan Guo, Kaiqiang Li, Guangyong Ye, Qingqing Lv, Juxing Zheng, Pan Chen, Tao Chen, Ke Hao, Kai Qian, Chengwen He, Zhen Wang",
journal="Journal of Zhejiang University Science D",
volume="9",
number="5",
pages="1023 - 1039",
year="2026",
publisher="Zhejiang University Press & Springer",
doi="10.1631/bdm.2600057"
}

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%A Youmin Ying
%A Xiaohuan Guo
%A Kaiqiang Li
%A Guangyong Ye
%A Qingqing Lv
%A Juxing Zheng
%A Pan Chen
%A Tao Chen
%A Ke Hao
%A Kai Qian
%A Chengwen He
%A Zhen Wang
%J Journal of Zhejiang University SCIENCE D
%V 9
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%P 1023 - 1039
%@ 1869-1951
%D 2026
%I Zhejiang University Press & Springer
%DOI 10.1631/bdm.2600057

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T1 - A dynamic non-invasive monitoring platform for multidimensional functional assessment of Kuanxiong aerosol against cardiac arrhythmias
A1 - Youmin Ying
A1 - Xiaohuan Guo
A1 - Kaiqiang Li
A1 - Guangyong Ye
A1 - Qingqing Lv
A1 - Juxing Zheng
A1 - Pan Chen
A1 - Tao Chen
A1 - Ke Hao
A1 - Kai Qian
A1 - Chengwen He
A1 - Zhen Wang
J0 - Journal of Zhejiang University Science D
VL - 9
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SP - 1023
EP - 1039
%@ 1869-1951
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PB - Zhejiang University Press & Springer
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DOI - 10.1631/bdm.2600057

Abstract
Chinese Summary
Academic Network
Reviewer Comment

Abstract: Arrhythmia is a critical clinical manifestation of cardiovascular disease; however, current therapeutic strategies are limited by significant side effects or high invasiveness. Kuanxiong aerosol (KXA), a traditional Chinese medicine (TCM) formulation clinically used for the relief of angina pectoris, exhibits multi-component and multi-target characteristics that suggest potential antiarrhythmic properties. However, direct cellular-level electrophysiological evidence is lacking. This study established an in vitro myocardial sensing model based on microelectrode array (MEA) technology. This platform supports non-invasive, real-time monitoring with parallel multi-site recording capability and has been used to systematically evaluate KXA’s intervention effects on various types of arrhythmias. We successfully constructed an MEA-based sensing platform using primary rat cardiomyocytes and determined a safe and effective KXA concentration. On this basis, we developed pathological models simulating chronic degenerative lesions and acute drug-induced bradycardia to assess the regulatory effects of KXA on myocardial electrical activity. Experimental results demonstrated that KXA effectively increased cardiomyocyte firing rate, enhanced electrical signal amplitudes, and restored the rhythmicity of calcium transients. This study reveals KXA’s multidimensional intervention effects on arrhythmias and successfully integrates advanced MEA biosensing technology into the dynamic pharmacodynamic evaluation of TCM compounds, providing an innovative bioengineering method for assessing the cardiac function of complex drug systems. Youmin Ying, Xiaohuan Guo, and Kaiqiang Li have contributed equally to this work.

一种用于宽胸气雾剂抗心律失常多维功能评价的动态无创监测平台

心律失常是心血管疾病的重要临床表现，现有治疗手段常受限于显著不良反应或高度侵入性。宽胸气雾剂（KXA）是临床用于缓解心绞痛的中药复方，其多成分与多靶点特性提示其可能具备抗心律失常作用，但目前尚缺乏细胞电生理层面的直接证据。本研究基于微电极阵列（MEA）技术构建了一种高通量、无创且可实时监测的体外心肌传感模型，系统评估了宽胸气雾剂对不同类型心律失常的干预效果。研究采用原代大鼠心肌细胞成功构建了基于微电极阵列的传感平台，并确定了宽胸气雾剂的安全有效浓度。在此基础上，建立了模拟慢性退行性病变和急性药物性心动过缓的病理模型，评估了宽胸气雾剂对心肌电活动的调节作用。实验结果表明，宽胸气雾剂能够有效提升心肌细胞的自发放电频率，改善电信号振幅，同时恢复钙瞬变的节律性。本研究不仅揭示了宽胸气雾剂对心律失常的多维度干预作用，更将先进的微电极阵列生物传感技术成功应用于中药复方的动态药效评价体系中，为复杂药物体系的心脏功能评估提供了一种创新的生物工程方法。
关键词：微电极阵列；实时监测；心律失常；宽胸气雾剂；心肌细胞