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Abstract: The molecular cloning of the G₁ genome segment of the Z₁₀ strain of hantaan virus and analysis of the first gene data on the currently widely used Hantavirus vaccine strain in China are presented. The G₁ genome segment of Z₁₀ virus was amplified by the method of RT-PCR, and the products were cloned into the pGEM-T vector after identification and purification. The clone was sequenced by Sanger's dideoxy chain termination method. The 1449 bases of the Z₁₀ G₁ genome segment ,and the coding for 483 amino acids were determined. The base compositions for the G₁ segment RNA determined from cDNA sequence information , were 20.6% A, 21.6% G, 18.8% C and 30.0% U. These values are similar to those of the 76/118, Lee and SR virus. As compared to the Z₁₀ G₁ segment, the sequence homology at the nucleotide level is 87%(76/118, type I), 86% (Lee, type I),86% (Hojo, type I), 67%(R22,type II), and 59%(K22, type III).The Z₁₀ virus G₁ protein amino acid sequence identity with other hantaan viruses (94-95% homology) was higher than that with Seoul type viruses (77-80%). More amino acid sequence heterogeneity between the Z₁₀ and 76/118 was observed in the N terminal, especially the diverging cluster of ten amino acids at position 84 to 93 of the Z₁₀ virus G₁ protein. Conclusion:(1) The Z₁₀ strain is one of the hantaan viruses. (2) The important region of the Z₁₀ G₁ segment was conservative. (3) Although substantial divergence of nucleotide sequences of the G₁ genome segment was found between the Z₁₀ strain and other type I Hantaviruses, relatively high amino acid sequence homology was shown among them. Thus, good immune protection could be obtained with the inactivated Meriones unguiculatus kidney cell vaccine against other strains of Hantaviruses.

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