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On-line Access: 2024-08-27
Received: 2023-10-17
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Water-dispersed bone morphogenetic protein nanospheres prepared by co-precipitation method
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JIANG Bing-bing, GAO Chang-you, HU Ling, SHEN Jia-cong. Water-dispersed bone morphogenetic protein nanospheres prepared by co-precipitation method[J]. Journal of Zhejiang University Science A, 2004, 5(8): 936-940.
@article{title="Water-dispersed bone morphogenetic protein nanospheres prepared by co-precipitation method",
author="JIANG Bing-bing, GAO Chang-you, HU Ling, SHEN Jia-cong",
journal="Journal of Zhejiang University Science A",
volume="5",
number="8",
pages="936-940",
year="2004",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.2004.0936"
}
%0 Journal Article
%T Water-dispersed bone morphogenetic protein nanospheres prepared by co-precipitation method
%A JIANG Bing-bing
%A GAO Chang-you
%A HU Ling
%A SHEN Jia-cong
%J Journal of Zhejiang University SCIENCE A
%V 5
%N 8
%P 936-940
%@ 1869-1951
%D 2004
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.2004.0936
TY - JOUR
T1 - Water-dispersed bone morphogenetic protein nanospheres prepared by co-precipitation method
A1 - JIANG Bing-bing
A1 - GAO Chang-you
A1 - HU Ling
A1 - SHEN Jia-cong
J0 - Journal of Zhejiang University Science A
VL - 5
IS - 8
SP - 936
EP - 940
%@ 1869-1951
Y1 - 2004
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.2004.0936
Abstract: A modified complex coacervation-co-precipitation method was used to prepare bone morphogenetic protein (BMP)-loaded nanospheres. Three natural polymers were used as packing materials to obtain nanoscale delivery device for BMP, in the presence of phosphatidylcholine functioning as stabilizer. Positively charged polysaccharide, N,N-diethylaminoethyl dextran (DEAE-dextran) tended to form stable, uniform and smaller size particles carrying BMP. Negatively charged bovine serum al-bumin (BSA) induced precipitation of the produced BMP particles due to its weak interaction with BMP molecules, although it produced nanosized BMP spheres. While collagen, a weakly positively charged protein shaped larger particles due to the strong interaction among themselves. A mechanism of co-precipitation process was also deduced to depict the formation of stable nanospheres.
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