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CLC number: O630
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Received: 2003-09-28
Revision Accepted: 2004-01-18
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Water-dispersed bone morphogenetic protein nanospheres prepared by co-precipitation method
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JIANG Bing-bing, GAO Chang-you, HU Ling, SHEN Jia-cong. Water-dispersed bone morphogenetic protein nanospheres prepared by co-precipitation method[J]. Journal of Zhejiang University Science A, 2004, 5(8): 936-940.
@article{title="Water-dispersed bone morphogenetic protein nanospheres prepared by co-precipitation method",
author="JIANG Bing-bing, GAO Chang-you, HU Ling, SHEN Jia-cong",
journal="Journal of Zhejiang University Science A",
volume="5",
number="8",
pages="936-940",
year="2004",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.2004.0936"
}
%0 Journal Article
%T Water-dispersed bone morphogenetic protein nanospheres prepared by co-precipitation method
%A JIANG Bing-bing
%A GAO Chang-you
%A HU Ling
%A SHEN Jia-cong
%J Journal of Zhejiang University SCIENCE A
%V 5
%N 8
%P 936-940
%@ 1869-1951
%D 2004
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.2004.0936
TY - JOUR
T1 - Water-dispersed bone morphogenetic protein nanospheres prepared by co-precipitation method
A1 - JIANG Bing-bing
A1 - GAO Chang-you
A1 - HU Ling
A1 - SHEN Jia-cong
J0 - Journal of Zhejiang University Science A
VL - 5
IS - 8
SP - 936
EP - 940
%@ 1869-1951
Y1 - 2004
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.2004.0936
Abstract: A modified complex coacervation-co-precipitation method was used to prepare bone morphogenetic protein (BMP)-loaded nanospheres. Three natural polymers were used as packing materials to obtain nanoscale delivery device for BMP, in the presence of phosphatidylcholine functioning as stabilizer. Positively charged polysaccharide, N,N-diethylaminoethyl dextran (DEAE-dextran) tended to form stable, uniform and smaller size particles carrying BMP. Negatively charged bovine serum al-bumin (BSA) induced precipitation of the produced BMP particles due to its weak interaction with BMP molecules, although it produced nanosized BMP spheres. While collagen, a weakly positively charged protein shaped larger particles due to the strong interaction among themselves. A mechanism of co-precipitation process was also deduced to depict the formation of stable nanospheres.
[1] Bessho, K., Carnes, D.L., Cavin, R., Ong, J.L., 2002. Experimental studies on bone induction using low-molecular-weight poly (DL-lactide-co-glycolide) as a carrier for recombinant human bone morphogenetic protein-2. Journal of Biomedical Materials Research, 61(1):61-65.
[2] Fessi, H., Puisieux, F., Devissaguet, J.P., Ammoury, N., Benita, S., 1989. Nanocapsule formation by interfacial deposition following solvent displacement. Int. J. Pharm., 55:1-4.
[3] Gao, C.Y., Wang, D.Y., Shen, J.C., 2003. Fabrication of porous collagen/chitosan scaffolds with controlling microstructure for dermal equivalent. Polymers for Advanced Technologies, 14(6):373-379.
[4] Groeneveld, E.H.J., Burger, E.H., 2000. Bone morphogenetic proteins in human bone regeneration. European Journal of Endocrinology, 142:9-21.
[5] King, G.N., King, N., Hughes, F.J., 1998. Effect of two delivery systems for recombinant human bone morphogenetic protein-2 on periodontal regeneration in vivo. Journal of Periodontal Research, 33(4):226-236.
[6] Kirker-Head, C.A., 2000. Potential applications and delivery strategies for bone morphogenetic proteins. Advanced Drug Delivery Review, 43:65-92.
[7] Urist, M.R., Sato, K., Brownell, A.G., 1983. Human Bone Morphogenetic Protein (hBMP). Proceedings of the Society for Experimental Biology and Medicine, 173:194-199.
[8] Winn, S.R., Hasan, U., Hollinger, J.O., 1998. Sustained release emphasizing recombinant human bone morphogenetic protein-2. Advanced Drug Delivery Reviews, 13:303-318.
[9] Woo, B.H., Fink, B.F., Page, R., Schrier, J.A., Jo, Y.W., Jiang, G., DeLuca, M., Vasconez, H.C., DeLuca, P.P., 2001. Enhancement of bone growth by sustained delivery of recombinant human bone morphogenetic protein-2 in a polymeric matrix. Pharmaceutical Research, 18(12):1747-1753.
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