Similar articles

Abstract: Vascular networks inside organs provide the means for metabolic exchange and adequate nutrition. Similarly, vascular or nutrient networks are needed when building tissue constructs >500 μm in vitro due to the hydrogel compact pore size of bioinks. As the hydrogel used in bioinks is rather soft, it is a great challenge to reconstruct effective vascular networks. Recently, coaxial 3D bioprinting was developed to print tissue constructs directly using hollow hydrogel fibers, which can be treated as built-in microchannels for nutrient delivery. Furthermore, vascular networks could be printed directly through coaxial 3D bioprinting. This review summarizes recent advances in coaxial bioprinting for the fabrication of complex vascularized tissue constructs including methods, the effectiveness of varying strategies, and the use of sacrificial bioink. The limitations and challenges of coaxial 3D bioprinting are also summarized.

同轴生物3D打印器官原型--从营养输送到血管化

[1]Abraham LC, Zuena E, Perez-Ramirez, et al., 2008. Guide to collagen characterization for biomaterial studies. Journal of Biomedical Materials Research-Part B Applied Biomaterials, 87B(1):264-285.

[2]Aguado BA, Mulyasasmita W, Su J, et al., 2012. Improving viability of stem cells during syringe needle flow through the design of hydrogel cell carriers. Tissue Engineering Part A, 18(7-8):806-815.

[3]Ashammakhi N, Ahadian S, Xu C, et al., 2019. Bioinks and bioprinting technologies to make heterogeneous and biomimetic tissue constructs. Materials Today Bio, 1:100008.

[4]Attalla R, Ling C, Selvaganapathy P, 2016. Fabrication and characterization of gels with integrated channels using 3D printing with microfluidic nozzle for tissue engineering applications. Biomedical Microdevices, 18(1):17.

[5]Axpe E, Oyen ML, 2016. Applications of alginate-based bioinks in 3D bioprinting. International Journal of Molecular Sciences, 17(12):1976.

[6]Bertassoni LE, Cecconi M, Manoharan V, et al., 2014. Hydrogel bioprinted microchannel networks for vascularization of tissue engineering constructs. Lab on a Chip, 14(13):2202-2211.

[7]Blaeser A, Duarte Campos DF, Fischer H, 2017. 3D bioprinting of cell-laden hydrogels for advanced tissue engineering. Current Opinion in Biomedical Engineering, 2:58-66.

[8]Chung JHY, Naficy S, Yue ZL, et al., 2013. Bio-ink properties and printability for extrusion printing living cells. Biomaterials Science, 1(7):763-773.

[9]Colosi C, Shin SR, Manoharan V, et al., 2016. Microfluidic bioprinting of heterogeneous 3D tissue constructs using low-viscosity bioink. Advanced Materials, 28(4):677-684.

[10]Cornelissen DJ, Faulkner-Jones A, Shu WM, 2017. Current developments in 3D bioprinting for tissue engineering. Current Opinion in Biomedical Engineering, 2:76-82.

[11]Costantini M, Colosi C, Świȩszkowski W, et al., 2018. Co-axial wet-spinning in 3D bioprinting: state of the art and future perspective of microfluidic integration. Biofabrication, 11(1):012001.

[12]Das S, Basu B, 2019. An overview of hydrogel-based bioinks for 3D bioprinting of soft tissues. Journal of the Indian Institute of Science, 99(3):405-428.

[13]Datta P, Ayan B, Ozbolat IT, 2017. Bioprinting for vascular and vascularized tissue biofabrication. Acta Biomaterialia, 51:1-20.

[14]Dolati F, Yu Y, Zhang YH, et al., 2014. In vitro evaluation of carbon-nanotube-reinforced bioprintable vascular conduits. Nanotechnology, 25(14):145101.

[15]Duarte Campos DF, Blaeser A, Buellesbach K, et al., 2016. Bioprinting organotypic hydrogels with improved mesenchymal stem cell remodeling and mineralization properties for bone tissue engineering. Advanced Healthcare Materials, 5(11):1336-1345.

[16]Gao Q, He Y, Fu JZ, et al., 2015. Coaxial nozzle-assisted 3D bioprinting with built-in microchannels for nutrients delivery. Biomaterials, 61:203-215.

[17]Gao Q, Liu ZJ, Lin ZW, et al., 2017. 3D bioprinting of vessel-like structures with multilevel fluidic channels. ACS Biomaterials Science & Engineering, 3(3):399-408.

[18]George M, Abraham TE, 2006. Polyionic hydrocolloids for the intestinal delivery of protein drugs: alginate and chitosan —a review. Journal of Controlled Release, 114(1):1-14.

[19]Glowacki J, Mizuno S, 2008. Collagen scaffolds for tissue engineering. Biopolymers, 89(5):338-344.

[20]Gómez-Guillén MC, Giménez B, López-Caballero ME, et al., 2011. Functional and bioactive properties of collagen and gelatin from alternative sources: a review. Food Hydrocolloids, 25(8):1813-1827.

[21]Griffith CK, Miller C, Sainson RCA, et al., 2005. Diffusion limits of an in vitro thick prevascularized tissue. Tissue Engineering, 11(1-2):257-266.

[22]Guvendiren M, Molde J, Soares RMD, et al., 2016. Designing biomaterials for 3D printing. ACS Biomaterials Science & Engineering, 2(10):1679-1693.

[23]Hann SY, Cui HT, Esworthy T, et al., 2019. Recent advances in 3D printing: vascular network for tissue and organ regeneration. Translational Research, 211:46-63.

[24]Haycock JW, 2011. 3D cell culture: a review of current approaches and techniques. In: Haycock JW (Ed.), 3D Cell Culture. Humana Press, New York, USA, p.1-15.

[25]He Y, Yang FF, Zhao HM, et al., 2016. Research on the printability of hydrogels in 3D bioprinting. Scientific Reports, 6:29977.

[26]He Y, Xie M, Gao Q, et al., 2019. Why choose 3D bioprinting? Part I: a brief introduction of 3D bioprinting for the beginners. Bio-Design and Manufacturing, 2:221-224.

[27]He Y, Gu Z, Xie M, et al., 2020. Why choose 3D bioprinting? Part II: methods and bioprinters. Bio-Design and Manufacturing, 3:1-4.

[28]Helary C, Bataille I, Abed A, et al., 2010. Concentrated collagen hydrogels as dermal substitutes. Biomaterials, 31(3):481-490.

[29]Hennink WE, van Nostrum CF, 2012. Novel crosslinking methods to design hydrogels. Advanced Drug Delivery Reviews, 64(S1):223-236.

[30]Hong S, Kim JS, Jung B, et al., 2019. Coaxial bioprinting of cell-laden vascular constructs using a gelatin-tyramine bioink. Biomaterials Science, 7(11):4578-4587.

[31]Ji S, Almeida E, Guvendiren M, 2019. 3D bioprinting of complex channels within cell-laden hydrogels. Acta Biomaterialia, 95:214-224.

[32]Jia WT, Gungor-Ozkerim PS, Zhang YS, et al., 2016. Direct 3D bioprinting of perfusable vascular constructs using a blend bioink. Biomaterials, 106:58-68.

[33]Kolesky DB, Truby RL, Gladman AS, et al., 2014. 3D bioprinting of vascularized, heterogeneous cell-laden tissue constructs. Advanced Materials, 26(19):3124-3130.

[34]Kuijpers AJ, van Wachem PB, van Luyn MJA, et al., 2000. In vivo compatibility and degradation of crosslinked gelatin gels incorporated in knitted Dacron. Journal of Biomedical Materials Research, 51(1):136-145.

[35]Kyle S, Jessop ZM, Al-Sabah A, et al., 2017. ‘Printability’ of candidate biomaterials for extrusion based 3D printing: state-of-the-art. Advanced Healthcare Materials, 6(16):1700264.

[36]Lee A, Hudson AR, Shiwarski DJ, et al., 2019. 3D bioprinting of collagen to rebuild components of the human heart. Science, 365(6452):482-487.

[37]Lee JM, Yeong WY, 2016. Design and printing strategies in 3D bioprinting of cell-hydrogels: a review. Advanced Healthcare Materials, 5(22):2856-2865.

[38]Lee JY, Koo YW, Kim GH, 2018. Innovative cryopreservation process using a modified core/shell cell-printing with a microfluidic system for cell-laden scaffolds. ACS Applied Materials & Interfaces, 10(11):9257-9268.

[39]Lee VK, Kim DY, Ngo H, et al., 2014. Creating perfused functional vascular channels using 3D bio-printing technology. Biomaterials, 35(28):8092-8102.

[40]Liu F, Chen QH, Liu C, et al., 2018. Natural polymers for organ 3D bioprinting. Polymers, 10(11):1278.

[41]Liu WJ, Heinrich MA, Zhou YX, et al., 2017. Extrusion bioprinting of shear-thinning gelatin methacryloyl bioinks. Advanced Healthcare Materials, 6(12):1601451.

[42]Liu WJ, Zhong Z, Hu N, et al., 2018. Coaxial extrusion bioprinting of 3D microfibrous constructs with cell-favorable gelatin methacryloyl microenvironments. Biofabrication, 10(2):024102.

[43]Madl CM, Katz LM, Heilshorn SC, 2016. Bio-orthogonally crosslinked, engineered protein hydrogels with tunable mechanics and biochemistry for cell encapsulation. Advanced Functional Materials, 26(21):3612-3620.

[44]Maiullari F, Costantini M, Milan M, et al., 2018. A multi-cellular 3D bioprinting approach for vascularized heart tissue engineering based on HUVECs and iPSC-derived cardiomyocytes. Scientific Reports, 8(1):13532.

[45]Mandrycky C, Wang ZJ, Kim K, et al., 2016. 3D bioprinting for engineering complex tissues. Biotechnology Advances, 34(4):422-434.

[46]McBeth C, Lauer J, Ottersbach M, et al., 2017. 3D bioprinting of GelMA scaffolds triggers mineral deposition by primary human osteoblasts. Biofabrication, 9(1):015009.

[47]Miller JS, Stevens KR, Yang MT, et al., 2012. Rapid casting of patterned vascular networks for perfusable engineered three-dimensional tissues. Nature Materials, 11(9):768-774.

[48]Mironov V, Trusk T, Kasyanov V, et al., 2009. Biofabrication: a 21st Century manufacturing paradigm. Biofabrication, 1(2):022001.

[49]Mistry P, Aied A, Alexander M, et al., 2017. Bioprinting using mechanically robust core–shell cell-laden hydrogel strands. Macromolecular Bioscience, 17(6):1600472.

[50]Murphy SV, Atala A, 2014. 3D bioprinting of tissues and organs. Nature Biotechnology, 32(8):773-785.

[51]Nagel T, Kelly DJ, 2013. The composition of engineered cartilage at the time of implantation determines the likelihood of regenerating tissue with a normal collagen architecture. Tissue Engineering Part A, 19(7-8):824-833.

[52]Ng WL, Chua CK, Shen YF, 2019. Print me an organ! why we are not there yet. Progress in Polymer Science, 97: 101145.

[53]Onoe H, Okitsu T, Itou A, et al., 2013. Metre-long cell-laden microfibres exhibit tissue morphologies and functions. Nature Materials, 12(6):584-590.

[54]Ouyang LL, Yao R, Zhao Y, et al., 2016. Effect of bioink properties on printability and cell viability for 3D bioplotting of embryonic stem cells. Biofabrication, 8(3):035020.

[55]Ouyang LL, Highley CB, Sun W, et al., 2017. A generalizable strategy for the 3D bioprinting of hydrogels from nonviscous photo-crosslinkable inks. Advanced Materials, 29(8):1604983.

[56]Ozbolat IT, Chen H, Yu Y, 2014. Development of ‘Multi-arm bioprinter’ for hybrid biofabrication of tissue engineering constructs. Robotics and Computer-Integrated Manufacturing, 30(3):295-304.

[57]Parenteau-Bareil R, Gauvin R, Berthod F, 2010. Collagen-based biomaterials for tissue engineering applications. Materials, 3(3):1863-1887.

[58]Park J, Lee SJ, Chung S, et al., 2017. Cell-laden 3D bioprinting hydrogel matrix depending on different compositions for soft tissue engineering: characterization and evaluation. Materials Science and Engineering: C, 71:678-684.

[59]Paulsen SJ, Miller JS, 2015. Tissue vascularization through 3D printing: will technology bring us flow? Developmental Dynamics, 244(5):629-640.

[60]Pawar SN, Edgar KJ, 2012. Alginate derivatization: a review of chemistry, properties and applications. Biomaterials, 33(11):3279-3305.

[61]Pereira RF, Bártolo PJ, 2015. 3D bioprinting of photocrosslinkable hydrogel constructs. Journal of Applied Polymer Science, 132(48):42458.

[62]Persikov AV, Ramshaw JAM, Kirkpatrick A, et al., 2005. Electrostatic interactions involving lysine make major contributions to collagen triple-helix stability. Biochemistry, 44(5):1414-1422.

[63]Pi QM, Maharjan S, Yan X, et al., 2018. Digitally tunable microfluidic bioprinting of multilayered cannular tissues. Advanced Materials, 30(43):1706913.

[64]Pinnock CB, Meier EM, Joshi NN, et al., 2016. Customizable engineered blood vessels using 3D printed inserts. Methods, 99:20-27.

[65]Radisic M, Yang LM, Boublik J, et al., 2004. Medium perfusion enables engineering of compact and contractile cardiac tissue. American Journal of Physiology-Heart and Circulatory Physiology, 286(2):H507-H516.

[66]Rouwkema J, Rivron NC, van Blitterswijk CA, 2008. Vascularization in tissue engineering. Trends in Biotechnology, 26(8):434-441.

[67]Rücker M, Laschke MW, Junker D, et al., 2006. Angiogenic and inflammatory response to biodegradable scaffolds in dorsal skinfold chambers of mice. Biomaterials, 27(29):5027-5038.

[68]Sasmal P, Datta P, Wu Y, et al., 2018. 3D bioprinting for modelling vasculature. Microphysiological Systems, 2:9.

[69]Sekine H, Shimizu T, Sakaguchi K, et al., 2013. In vitro fabrication of functional three-dimensional tissues with perfusable blood vessels. Nature Communications, 4:1399.

[70]Shao L, Gao Q, Zhao HM, et al., 2018. Fiber-based mini tissue with morphology-controllable gelma microfibers. Small, 14(44):1802187.

[71]Shao L, Gao Q, Xie CQ, et al., 2019. Bioprinting of cell-laden microfiber: can it become a standard product? Advanced Healthcare Materials, 8(9):1900014.

[72]Shao L, Gao Q, Xie CQ, et al., 2020a. Directly coaxial 3D bioprinting of large-scale vascularized tissue constructs. Biofabrication, 12(3):035014.

[73]Shao L, Gao Q, Xie CQ, et al., 2020b. Synchronous 3D bioprinting of large-scale cell-laden constructs with nutrient networks. Advanced Healthcare Materials, 9(15):1901142.

[74]Shaw CJ, Ter Haar GR, Rivens IH, et al., 2014. Pathophysiological mechanisms of high-intensity focused ultrasound-mediated vascular occlusion and relevance to non-invasive fetal surgery. Journal of the Royal Society Interface, 11(95):20140029.

[75]Spang MT, Christman KL, 2018. Extracellular matrix hydrogel therapies: in vivo applications and development. Acta Biomaterialia, 68:1-14.

[76]Suntornnond R, An J, Yeong WY, et al., 2015. Biodegradable polymeric films and membranes processing and forming for tissue engineering. Macromolecular Materials and Engineering, 300(9):858-877.

[77]Suntornnond R, An J, Chua CK, 2017. Bioprinting of thermoresponsive hydrogels for next generation tissue engineering: a review. Macromolecular Materials and Engineering, 302(1):1600266.

[78]Townsend JM, Beck EC, Gehrke SH, et al., 2019. Flow behavior prior to crosslinking: the need for precursor rheology for placement of hydrogels in medical applications and for 3D bioprinting. Progress in Polymer Science, 91:126-140.

[79]Unagolla JM, Jayasuriya AC, 2020. Hydrogel-based 3D bioprinting: a comprehensive review on cell-laden hydrogels, bioink formulations, and future perspectives. Applied Materials Today, 18:100479.

[80]van den Bulcke AI, Bogdanov B, de Rooze N, et al., 2000. Structural and rheological properties of methacrylamide modified gelatin hydrogels. Biomacromolecules, 1(1):31-38.

[81]Wang XH, He K, Zhang WM, 2013. Optimizing the fabrication processes for manufacturing a hybrid hierarchical polyurethane-cell/hydrogel construct. Journal of Bioactive and Compatible Polymers, 28(4):303-319.

[82]Wang XH, Ao Q, Tian XH, et al., 2017. Gelatin-based hydrogels for organ 3D bioprinting. Polymers, 9(9):401.

[83]Wu ZJ, Su X, Xu YY, et al., 2016. Bioprinting three-dimensional cell-laden tissue constructs with controllable degradation. Scientific Reports, 6:24474.

[84]Xie M, Gao Q, Fu J, et al., 2020a. Bioprinting of novel 3D tumor array chip for drug screening. Bio-Design and Manufacturing, 3:175-188.

[85]Xie M, Zheng Y, Gao Q, et al., 2020b. Facile 3D cell culture protocol based on photocurable hydrogels. Bio-Design and Manufacturing, in press.

[86]Xing Q, Yates K, Vogt C, et al., 2014. Increasing mechanical strength of gelatin hydrogels by divalent metal ion removal. Scientific Reports, 4:4706.

[87]Yao R, Zhang RJ, Yan YN, et al., 2009. In vitro angiogenesis of 3D tissue engineered adipose tissue. Journal of Bioactive and Compatible Polymers, 24(1):5-24.

[88]Yeo MG, Lee JS, Chun W, et al., 2016. An innovative collagen-based cell-printing method for obtaining human adipose stem cell-laden structures consisting of core-sheath structures for tissue engineering. Biomacromolecules, 17(4):1365-1375.

[89]Yu Y, Zhang YH, Martin JA, et al., 2013. Evaluation of cell viability and functionality in vessel-like bioprintable cell-laden tubular channels. Journal of Biomechanical Engineering, 135(9):091011.

[90]Zhang LW, Li GY, Shi M, et al., 2017. Establishment and characterization of an acute model of ocular hypertension by laser-induced occlusion of episcleral veins. Investigative Ophthalmology & Visual Science, 58(10):3879-3886.

[91]Zhang Y, Zhou DZ, Chen JW, et al., 2019. Biomaterials based on marine resources for 3D bioprinting applications. Marine Drugs, 17(10):555.

[92]Zhang YH, Yu Y, Chen H, et al., 2013. Characterization of printable cellular micro-fluidic channels for tissue engineering. Biofabrication, 5(2):025004.

[93]Zhang YH, Yu Y, Akkouch A, et al., 2015. In vitro study of directly bioprinted perfusable vasculature conduits. Biomaterials Science, 3(1):134-143.

[94]Zhang YS, Arneri A, Bersini S, et al., 2016. Bioprinting 3D microfibrous scaffolds for engineering endothelialized myocardium and heart-on-a-chip. Biomaterials, 110: 45-59.