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Abstract: Event-related potential (ERP) is a reliable neuroelectric measure of brain activity that helps to confirm the assessment of mental status and cognitive impairment. Many studies have reported that alcoholics show a significantly lower ERP P300 amplitude than the norm. In the present study, ERP P300 waves were measured to evaluate the effect of citric acid on cognitive function during excessive alcohol consumption in healthy adults. Five volunteers were selected through clinical interview, physical examination, and psychiatric assessment for participation in this study. In a double-blind placebo-controlled before-after design, each subject was treated with 5 ml/kg body weight alcohol, 5 ml/kg body weight alcohol and 1 mg citric acid, or a placebo on three separate occasions, one week apart. ERP P300, blood biochemical indicators, blood alcohol concentrations (BACs) and acetaldehyde concentrations were assessed. Repeated measure analysis of variance (ANOVA) with a within-subjects factor was used to evaluate differences in blood biochemical indicators, BACs, blood acetaldehyde concentrations, and ERP P300 in the three sessions of assessments. Several blood biochemical indicators showed significant differences between treatments, including the levels of cholinesterase (CHE), total bile acid (TBA), triglyceride (TG), total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C), very low-density lipoprotein cholesterol (VLDL-C), and glycylproline dipeptidyl aminopeptidase (GPDA). BACs after consumption of alcohol alone or citric acid with alcohol were significantly higher compared to those after placebo treatment (P<0.05). There were no significant differences in blood acetaldehyde concentrations between the treatments. The P300 amplitudes on the frontal (Fz), central (Cz), and parietal (Pz) regions of the scalp after consumption of alcohol were significantly lower than those after consumption of the placebo or citric acid with alcohol (P<0.05), while there were no significant differences between the latter two treatments. The results of this study suggest that citric acid could reduce the decline in ERP P300 amplitude and cognitive ability induced by acute alcohol consumption. It may also affect some blood biochemical indicators, but the specific mechanisms need further research.

[1]Barrett, G., Neshige, R., Shibasaki, H., 1987. Human auditory and somatosensory event-related potentials: effects of response condition and age. Electroencephalogr. Clin. Neurophysiol., 66(4):409-419.

[2]Bowden, S.C., McCarter, R.J., 1993. Spatial memory in alcohol-dependent subjects: using a push-button maze to test the principle of equiavailability. Brain Cogn., 22(1):51-62.

[3]Cippitelli, A., Damadzic, R., Frankola, K., Goldstein, A., Thorsell, A., Singley, E., Eskay, R.L., Heilig, M., 2010. Alcohol-induced neurodegeneration, suppression of transforming growth factor-beta, and cognitive impairment in rats: prevention by group II metabotropic glutamate receptor activation. Biol. Psychiatry, 67(9):823-830.

[4]Cloninger, C.R., 1987. Neurogenetic adaptive mechanisms in alcoholism. Science, 236(4800):410-416.

[5]Correa, M., Manrique, H.M., Font, L., Escrig, M.A., Aragon, C.M., 2008. Reduction in the anxiolytic effects of ethanol by centrally formed acetaldehyde: the role of catalase inhibitors and acetaldehyde-sequestering agents. Psychopharmacology (Berl), 200(4):455-464.

[6]Correa, M., Arizzi-Lafrance, M.N., Salamone, J.D., 2009. Infusions of acetaldehyde into the arcuate nucleus of the hypothalamus induce motor activity in rats. Life Sci., 84(11-12):321-327.

[7]Correa, M., Salamone, J.D., Segovia, K.N., Pardo, M., Longoni, R., Spina, L., Peana, A.T., Vinci, S., Acquas, E., 2012. Piecing together the puzzle of acetaldehyde as a neuroactive agent. Neurosci. Biobehav. Rev., 36(1):404-430.

[8]Fonseca, L.L., Alves, P.M., Carrondo, M.J., Santos, H., 2001. Effect of ethanol on the metabolism of primary astrocytes studied by ¹³C- and ³¹P-NMR spectroscopy. J. Neurosci. Res., 66(5):803-811.

[9]Gaillard, A.W., 1988. Problems and paradigms in ERP research. Biol. Psychol., 26(1-3):91-109.

[10]Haorah, J., Ramirez, S.H., Floreani, N., Gorantla, S., Morsey, B., Persidsky, Y., 2008. Mechanism of alcohol-induced oxidative stress and neuronal injury. Free Radic. Biol. Med., 45(11):1542-1550.

[11]Isreal, J.B., Chesney, G.L., Wickens, C.D., Donchin, E., 1980. P300 and tracking difficulty: evidence for multiple resources in dual-task performance. Psychophysiology, 17(3):259-273.

[12]Jääskeläiinen, I.P., Näätänen, R., Sillanaukee, P., 1996. Effect of acute ethanol on auditory and visual event-related potentials: a review and reinterpretation. Biol. Psychiatry, 40(4):284-291.
[doi:10.1016/0006-3223(95)00385-1]

[13]Li, X., Shao, X., Wang, N., Wang, T., Chen, G., Zhou, H., 2010. Correlation of auditory event-related potentials and magnetic resonance spectroscopy measures in mild cognitive impairment. Brain Res., 1346:204-212.

[14]Liguori, A., Robinson, J.H., 2001. Caffeine antagonism of alcohol induced driving impairment. Drug Alcohol Depend., 63(2):123-129.

[15]Martin, F.H., Siddle, D.A., 2003. The interactive effects of alcohol and temazepam on P300 and reaction time. Brain Cogn., 53(1):58-65.

[16]Meshitsuka, S., Aremu, D.A., 2008. ¹³C heteronuclear NMR studies of the interaction of cultured neurons and astrocytes and aluminum blockade of the preferential release of citrate from astrocytes. J. Biol. Inorg. Chem., 13(2):241-247.

[17]Miyazato, Y., Ogura, C., 1993. Abnormalities in event-related potentials: N100, N200 and P300 topography in alcoholics. Jpn. J. Psychiatry Neurol., 47(4):853-862.

[18]Moselhy, H.F., Georgiou, G., Kahn, A., 2001. Frontal lobe changes in alcoholism: a review of the literature. Alcohol Alcohol., 36(5):357-368.

[19]Oscar-Berman, M., 1987. Alcohol-related ERP changes in cognition. Alcohol, 4(4):289-292.

[20]Pfefferbaum, A., Rosenbloom, M., Ford, J.M., 1987. Late event-related potential changes in alcoholics. Alcohol, 4(4):275-281.

[21]Polich, J., Ladish, C., Bloom, F.E., 1990. P300 assessment of early Alzheimer’s disease. Electroencephalogr. Clin. Neurophysiol., 77(3):179-189.

[22]Shin, H.Y., Shin, I.S., Yoon, J.S., 2006. ALDH2 genotype-associated differences in the acute effects of alcohol on P300, psychomotor performance, and subjective response in healthy young Korean men: a double-blind placebo-controlled crossover study. Hum. Psychopharmacol., 21(3):159-166.

[23]Tuck, R.R., Jackson, M., 1991. Social, neurological and cognitive disorders in alcoholics. Med. J. Aust., 155(4):225-229.

[24]Wester, A.E., Verster, J.C., Volkerts, E.R., Böcker, K.B., Kenemans, J.L., 2010. Effects of alcohol on attention orienting and dual-task performance during simulated driving: an event-related potential study. J. Psychopharmacol., 24(9):1333-1348.

[25]Williamson, J.R., Scholz, R., Browning, E.T., Thurman, R.G., Fukami, M.H., 1969. Metabolic effects of ethanol in perfused rat liver. J. Biol. Chem., 244(18):5044-5054.

[26]Zucker, R.A., Donovan, J.E., Masten, A.S., Mattson, M.E., Moss, H.B., 2008. Early developmental processes and the continuity of risk for underage drinking and problem drinking. Pediatrics, 121(S4):S252-S272.