Similar articles

Abstract: Objective: To investigate the protective effects and mechanisms of action of b%5D=abstract&ck%5b%5D=keyword'>b>dexamethasone and b%5D=abstract&ck%5b%5D=keyword'>b>Salvia miltiorrhiza on b%5D=abstract&ck%5b%5D=keyword'>b>multiple organs in b%5D=abstract&ck%5b%5D=keyword'>b>rats with severe acute b%5D=abstract&ck%5b%5D=keyword'>b>pancreatitis (SAP). Methods: The b%5D=abstract&ck%5b%5D=keyword'>b>rats were divided into sham-operated, model control, b%5D=abstract&ck%5b%5D=keyword'>b>dexamethasone treated, and b%5D=abstract&ck%5b%5D=keyword'>b>Salvia miltiorrhiza treated groups. At 3, 6, and 12 h after operation, the mortality rate of different groups, pathological changes, bcl-2-associated X protein (bax) and nuclear factor-bA;b (bA%29&ck%5b%5D=abstract&ck%5b%5D=keyword'>b>NF-bA;b) protein expression levels in b%5D=abstract&ck%5b%5D=keyword'>b>multiple organs (the pancreas, liver, kidneys, and lungs), toll-like receptor 4 (TLR-4) protein levels (only in the liver), intercellular adhesion molecule 1 (ICAM-1) protein levels (only in the lung), and terminal deoxynucleotidy transferase mediated deoxyuridine triphosphate (dUTP) nick end labeling (TUNEL) staining expression levels, as well as the serum contents of amylase, glutamate-pyruvate transaminase (GPT), glutamic-oxaloacetic transaminase (GOT), blood urea nitrogen (bUN), and creatinine (CREA) were observed. Results: The mortality rate of the b%5D=abstract&ck%5b%5D=keyword'>b>dexamethasone treated group was significantly lower than that of the model control group (P<0.05). The pathological changes in b%5D=abstract&ck%5b%5D=keyword'>b>multiple organs in the two treated groups were relieved to different degrees (P<0.05 and P<0.01, respectively), the expression levels of bax and bA%29&ck%5b%5D=abstract&ck%5b%5D=keyword'>b>NF-bA;b proteins, and apoptotic indexes of b%5D=abstract&ck%5b%5D=keyword'>b>multiple organs were reduced (P<0.05 and P<0.01, respectively). The contents of amylase, GPT, GOT, bUN, and CREA in the two treated groups were significantly lower than those in model control groups (P<0.05 and P<0.01, respectively). The expression level of ICAM-1 protein in the lungs (at 3 and 12 h) in the b%5D=abstract&ck%5b%5D=keyword'>b>dexamethasone treated group was significantly lower than that in the b%5D=abstract&ck%5b%5D=keyword'>b>Salvia miltiorrhiza treated group (P<0.05). The serum contents of CREA (at 12 h) and bUN (at 6 h) of the b%5D=abstract&ck%5b%5D=keyword'>b>Salvia miltiorrhiza treated group were significantly lower than those in the b%5D=abstract&ck%5b%5D=keyword'>b>dexamethasone treated group (P<0.05). Conclusions: both b%5D=abstract&ck%5b%5D=keyword'>b>dexamethasone and b%5D=abstract&ck%5b%5D=keyword'>b>Salvia miltiorrhiza can reduce the inflammatory reaction, regulate b%5D=abstract&ck%5b%5D=keyword'>b>apoptosis, and thus protect b%5D=abstract&ck%5b%5D=keyword'>b>multiple organs of b%5D=abstract&ck%5b%5D=keyword'>b>rats with SAP.

[1]Campo, G.M., Avenoso, A., Campo, S., Nastasi, G., Traina, P., D(Ascola, A., Calatroni, A., 2008. Chondroitin-4-sulphate reduced oxidative injury in caerulein-induced pancreatitis in mice: the involvement of NF-kappaB translocation and apoptosis activation. Exp. Biol. Med. (Maywood), 233(6):741-752.

[2]Cui, Y., Li, Y., Shi, W., Yang, M., Zhao, X., Xia, Z., 2007. Separation of water-soluble active components in Salvia miltiorrhiza bge. f. alba using capillary zone electrophoresis. Se Pu, 25(5):686-689 (in Chinese).

[3]Ding, M., Yuan, Y.J., 2007. Study on the mechanisms of an extract of Salvia miltiorrhiza on the regulation of permeability of endothelial cells exposed to tumor necrosis factor-α. J. Pharm. Pharmacol., 59(7):1027-1033.

[4]Foitzik, T., Eibl, G., Hotz, H.G., Faulhaber, J., Kirchengast, M., Buhr, H.J., 2000. Endothelin receptor blockade in severe acute pancreatitis leads to systemic enhancement of capillary permeability and improved survival rates. Surgery, 128(3):399-407.

[5]Gomez, G., Lee, H.M., He, Q., Englander, E.W., Uchida, T., Greeley, G.H.Jr., 2001. Acute pancreatitis signals activation of apoptosis-associated and survival genes in mice. Exp. Biol. Med. (Maywood), 226(7):692-700.

[6]Kandil, E., Lin, Y.Y., Bluth, M.H., Zhang, H., Levi, G., Zenilman, M.E., 2006. Dexamethasone mediates protection against acute pancreatitis via up-regulation of pancreatitis-associated proteins. World J. Gastroenterol., 12(42):6806-6811.

[7]Kim, J.S., Narula, A.S., Jobin, C., 2005. Salvia miltiorrhiza water-soluble extract, but not its constituent salvianolic acid B, abrogates LPS-induced NF-kappaB signaling in intestinal epithelial cells. Clin. Exp. Immunol., 141(2):288-297.

[8]Lam, F.F., Yeung, J.H., Chan, K.M., 2007. Relaxant effects of Salvia miltiorrhiza aqueous extract and its constituent danshensu on rat coronary artery are mediated by inhibition of calcium channels. Vascul. Pharmacol., 46(4):271-277.

[9]Li, D., Xiong, S.D., Du, D.B., 2006. Inhibitory effects of Salvia miltiorrhiza injection coordinated with dexamethasone on interleukin-13 and eotaxin expression in lung of asthmatic rats. Chin. J. Int. Trad. West. Med., 26(11):1007-1110 (in Chinese).

[10]Martín Alonso, M.A., Santamaría, A., Saracíbar, E., Arranz, E., Garrote, J.A., Almaraz, A., Caro-Patón, A., 2007. Cytokines and other immunological parameters as markers of distant organ involvement in acute pancreatitis. Med. Clin. (Barc.), 128(11):401-406.

[11]Meng, Y., Ma, Q.Y., Kou, X.P., Xu, J., 2005. Effect of resveratrol on activation of nuclear factor kappa-B and inflammatory factors in rat model of acute pancreatitis. World J. Gastroenterol., 11(4):525-528.

[12]Mizuguchi, T., Mukaiya, M., Imaizumi, H., Kimura, Y., Masuda, Y., Katsuramaki, T., Asai, Y., Hirata, K., 2004. Successful management of severe acute pancreatitis with multiple organ failure. Pancreas, 28(2):211-213.

[13]Muller, C.A., Belyaev, O., Appelros, S., Buchler, M., Uhl, W., Borgstrom, A., 2008. Dexamethasone affects inflammation but not trypsinogen activation in experimental acute pancreatitis. Eur. Surg. Res., 40(4):317-324.

[14]Qu, J.G., Zhang, J.X., Cheng, G.Z., 2003. Effect of urinastatin on microcirculation of extrapancreatic organs in rats with acute necrotizing pancreatitis. Chin. J. Bases Clin. General Surg., 10(3):234-235 (in Chinese).

[15]Samuilov, V.D., Oleskin, A.V., Lagunova, E.M., 2000. Programmed cell death. Biochemistry (Mosc.), 65(8):873-887.

[16]Sha, H., Ma, Q., Jha, R.K., Xu, F., Wang, L., Wang, Z., Zhao, Y., Fan, F., 2008. Resveratrol ameliorates hepatic injury via the mitochondrial pathway in rats with severe acute pancreatitis. Eur. J. Pharmacol., 601(1-3):136-142.

[17]Song, J.M., Liu, H.X., Li, Y., Zeng, Y.J., Zhou, Z.G., Liu, H.Y., 2008. Extracellular heat-shock protein 70 aggravates cerulein-induced pancreatitis through toll-like receptor-4 in mice. Chin. Med. J. (Engl.), 121(15):1420-1425.

[18]Strate, T., Mann, O., Kleinhans, H., Schneider, C., Knoefel, W.T., Yekebas, E., Standl, T., Bloechle, C., Izbicki, J.R., 2003. Systemic intravenous infusion of bovine hemoglobin significantly reduces microcirculatory dysfunction in experimentally induced pancreatitis in the rat. Ann. Surg., 238(5):765-771.

[19]Sugiyama, Y., Kato, S., Abe, M., Mitsufuji, S., Takeuchi, K., 2005. Different effects of dexamethasone and the nitric oxide synthase inhibitor L-NAME on caerulein-induced rat acute pancreatitis, depending on the severity. Inflammopharmacology, 13(1-3):291-301.

[20]Takeyama, Y., 2005. Significance of apoptotic cell death in systemic complications with severe acute pancreatitis. J. Gastroenterol., 40(1):1-10.

[21]von Dobschuetz, E., Pahernik, S., Hoffmann, T., Kiefmann, R., Heckel, K., Messmer, K., Mueller-Hoecker, J., Dellian, M., 2004. Dynamic intravital fluorescence microscopy— a novel method for the assessment of microvascular permeability in acute pancreatitis. Microvasc. Res., 67(1):55-63.

[22]Xu, P., Zhou, X.J., Chen, L.Q., Chen, J., Xie, Y., Lv, L.H., Hou, X.H., 2007. Pioglitazone attenuates the severity of sodium taurocholate-induced severe acute pancreatitis. World J. Gastroenterol., 13(13):1983-1988.

[23]Zhang, J.X., Cheng, G.Z., Li, L., Qu, J.G., 2002a. The effect of labiatae on changes of gastric mucosal microcirculation in rats with acute necrotizing pancreatitis. Jiangsu Monthly J., 28(7):496-498 (in Chinese).

[24]Zhang, J.X., Cheng, G.Z., Li, L., Qu, J.G., 2002b. The effect and protection of labiatae on intestine microcirculation in rats with acute pancreatitis. Chin. J. Hepatobiliary Surg., 8(12):753-754 (in Chinese).

[25]Zhang, J.X., Cheng, G.Z., Li, L., Cheng, J.X., Zhang, Q., 2002c. The effect of labiatae on acute necrotizing pancreatitis in rats with lung injury. Chin. J. Emerg. Med., 11(2):101-104 (in Chinese).

[26]Zhang, J.X., Qu, J.G., Li, L., Xie, R., Cheng, G.Z., 2003. The effect of labiatae on renal injury in rats with acute necrotizing pancreatitis. Chin. J. Emerg. Med., 12(2):97-102 (in Chinese).

[27]Zhang, X., Chen, L., Luo, L., Tian, H., Feng, G., Cai, Y., Xu, R., Wang, K., Wang, Z., 2008. Study of the protective effects of dexamethasone on ileum mucosa injury in rats with severe acute pancreatitis. Pancreas, 37(3):e74-e82.

[28]Zhang, X.P., Li, Z.J., Liu, D.R., 2006. Progress in research into the mechanism of radix Salviae miltiorrhizae in treatment of acute pancreatitis. Hepatobiliary Pancreat Dis. Int., 5(4):501-504.

[29]Zhang, X.P., Lin, Q., Zhou, Y.F., 2007a. Progress of study on the relationship between mediators of inflammation and apoptosis in acute pancreatitis. Dig. Dis. Sci., 52(5):1199-1205.

[30]Zhang, X.P., Ye, Q., Jiang, X.G., Ma, M.L., Zhu, F.B., Zhang, R.P., Cheng, Q.H., 2007b. Preparation method of an ideal model of multiple organ injury of rat with severe acute pancreatitis. World J. Gastroenterol., 13(34):4566-4573.

[31]Zhang, X.P., Zhang, L., Wang, Y., Cheng, Q.H., Wang, J.M., Cai, W., Shen, H.P., Cai, J., 2007c. Study of the protective effects of dexamethasone on multiple organ injury in rats with severe acute pancreatitis. JOP, 8(4):400-412.

[32]Zhang, X.P., Zhang, L., Chen, L.J., Cheng, Q.H., Wang, J.M., Cai, W., Shen, H.P., Cai, J., 2007d. Influence of dexamethasone on inflammatory mediators and NF-kappaB expression in multiple organs of rats with severe acute pancreatitis. World J. Gastroenterol., 13(47):6385-6395.

[33]Zhang, X.P., Chen, L., Hu, Q.F., Tian, H., Xu, R.J., Wang, Z.W., Wang, K.Y., Cheng, Q.H., Yan, W., Li, Y., et al., 2007e. Effects of large dose of dexamethasone on inflammatory mediators and pancreatic cell apoptosis of rats with severe acute pancreatitis. World J. Gastroenterol., 13(41):5506-5511.

[34]Zhang, X.P., Xu, H.M., Jiang, Y.Y., Yu, S., Cai, Y., Lu, B., Xie, Q., Ju, T.F., 2008. Influence of dexamethasone on mesenteric lymph node of rats with severe acute pancreatitis. World J. Gastroenterol., 14(22):3511-3517.

[35]Zhang, X.P., Pan,Y., Huang, X.M., Feng, G.H., Ma, M.L., Ni, J., Zhang, F.J., 2010. Effects of dexamethasone and Salvia miltiorrhiza on the small intestine and immune organs of rats with severe acute pancreatitis. Inflammation, 33(4):259-266.

[36]Zhang, Y., Shi, C.X., Li, Y.X., 2005. Effect of Salvia miltiorrhiza on mRNA expression of inducible nitric oxide synthase and organ injury in rats with severe acute pancreatitis. Chin. J. Int. Trad. West. Med., 25(11):1012-1015 (in Chinese).

[37]Zhao, G.R., Zhang, H.M., Ye, T.X., 2008. Characterization of the radical scavenging and antioxidant activities of danshensu and salvianolic acid B. Food Chem. Toxicol., 46(1):73-81.