Journal of Zhejiang University

Journal of Zhejiang University SCIENCE B 2014 Vol.15 No.3 P.272-280

Buyang Huanwu decoction increases angiopoietin-1 expression and promotes angiogenesis and functional outcome after focal cerebral ischemia*

Author(s): Jian Shen¹,Yu Zhu¹,Hai Yu¹,Zuo-xu Fan¹,Feng Xiao¹,Pan Wu¹,Qi-hui Zhang²,Xiao-xing Xiong³,Jian-wei Pan¹,Ren-ya Zhan¹
Affiliation(s): 1. 1. Brain Medicine Center, the First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310003, China more
Corresponding email(s): neurosurg_zju@yeah.net
Key Words: Buyang Huanwu decoction, Angiopoietin-1, Angiogenesis, Neurorestoration, Chronic brain injury

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Jian Shen, Yu Zhu, Hai Yu, Zuo-xu Fan, Feng Xiao, Pan Wu, Qi-hui Zhang, Xiao-xing Xiong, Jian-wei Pan, Ren-ya Zhan. Buyang Huanwu decoction increases angiopoietin-1 expression and promotes angiogenesis and functional outcome after focal cerebral ischemia[J]. Journal of Zhejiang University Science B, 2014, 15(3): 272-280.

@article{title="Buyang Huanwu decoction increases angiopoietin-1 expression and promotes angiogenesis and functional outcome after focal cerebral ischemia",
author="Jian Shen, Yu Zhu, Hai Yu, Zuo-xu Fan, Feng Xiao, Pan Wu, Qi-hui Zhang, Xiao-xing Xiong, Jian-wei Pan, Ren-ya Zhan",
journal="Journal of Zhejiang University Science B",
volume="15",
number="3",
pages="272-280",
year="2014",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1300166"
}

%0 Journal Article
%T Buyang Huanwu decoction increases angiopoietin-1 expression and promotes angiogenesis and functional outcome after focal cerebral ischemia
%A Jian Shen
%A Yu Zhu
%A Hai Yu
%A Zuo-xu Fan
%A Feng Xiao
%A Pan Wu
%A Qi-hui Zhang
%A Xiao-xing Xiong
%A Jian-wei Pan
%A Ren-ya Zhan
%J Journal of Zhejiang University SCIENCE B
%V 15
%N 3
%P 272-280
%@ 1673-1581
%D 2014
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1300166

TY - JOUR
T1 - Buyang Huanwu decoction increases angiopoietin-1 expression and promotes angiogenesis and functional outcome after focal cerebral ischemia
A1 - Jian Shen
A1 - Yu Zhu
A1 - Hai Yu
A1 - Zuo-xu Fan
A1 - Feng Xiao
A1 - Pan Wu
A1 - Qi-hui Zhang
A1 - Xiao-xing Xiong
A1 - Jian-wei Pan
A1 - Ren-ya Zhan
J0 - Journal of Zhejiang University Science B
VL - 15
IS - 3
SP - 272
EP - 280
%@ 1673-1581
Y1 - 2014
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1300166

Abstract
Chinese Summary
Academic Network
Reviewer Comment

Abstract: buyang Huanwu decoction (BYHWD), a traditional Chinese herbal prescription, has been widely used clinically to treat stroke in China for hundreds of years; however, the mechanisms of this drug for stroke treatment are still unclear. This study aims to observe the cerebral angiogenesis effects of BYHWD on chronic brain injury after focal cerebral ischemia in rats and to explore its possible mechanisms. The ischemia was induced by occlusion of the right middle cerebral artery for 90 min. BYHWD (12.5 and 25.0 g/(kg∙d), equivalent to the dry weight of the raw materials) was orally administered twice a day beginning 2 h after surgery. BYHWD significantly attenuated the neurological dysfunction, infarct volume, and brain atrophy after ischemia. There was a significant increase in the microvessel density, as assessed by immunofluorescence CD31, and a significant increase in angiopoietin-1 (Ang-1) in the penumbra areas of the rats was shown by immunohistochemical staining and Western blotting. The results indicate that the neurorestorative effects of BYHWD are associated with angiogenesis and the enhancement of the expressions of Ang-1 on chronic brain injury after focal cerebral ischemia.

补阳还五汤增加脑缺血后血管生成素-1的表达及促进血管生成和神经功能改善

研究目的：补阳还五汤对脑缺血后缺血区血管生成的影响及其机制。
创新要点：研究结果表明补阳还五汤可能通过增加血管生成素-1的表达，促进缺血区血管新生，从而促进脑梗死后神经功能恢复。
研究方法：通过神经功能评分、脑梗死及脑萎缩体积评估神经功能恢复情况；通过免疫组化及免疫印迹等方法检测血管密度及血管生成因子的表达情况。
重要结论：补阳还五汤增加脑缺血后血管生成素-1的表达，能够促进脑梗死缺血区血管生成。

关键词：补阳还五汤；血管生成素-1；血管生成；慢性脑损伤；神经修复