Improvement of a gene targeting system for genetic manipulation in Penicillium digitatum
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Qian Xu, Cong-yi Zhu, Ming-shang Wang, Xue-peng Sun, Hong-ye Li. Improvement of a gene targeting system for genetic manipulation in Penicillium digitatum[J]. Journal of Zhejiang University Science B, 2014, 15(2): 116-124.
@article{title="Improvement of a gene targeting system for genetic manipulation in Penicillium digitatum",
author="Qian Xu, Cong-yi Zhu, Ming-shang Wang, Xue-peng Sun, Hong-ye Li",
journal="Journal of Zhejiang University Science B",
volume="15",
number="2",
pages="116-124",
year="2014",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1300213"
}
%0 Journal Article
%T Improvement of a gene targeting system for genetic manipulation in Penicillium digitatum
%A Qian Xu
%A Cong-yi Zhu
%A Ming-shang Wang
%A Xue-peng Sun
%A Hong-ye Li
%J Journal of Zhejiang University SCIENCE B
%V 15
%N 2
%P 116-124
%@ 1673-1581
%D 2014
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1300213
TY - JOUR
T1 - Improvement of a gene targeting system for genetic manipulation in Penicillium digitatum
A1 - Qian Xu
A1 - Cong-yi Zhu
A1 - Ming-shang Wang
A1 - Xue-peng Sun
A1 - Hong-ye Li
J0 - Journal of Zhejiang University Science B
VL - 15
IS - 2
SP - 116
EP - 124
%@ 1673-1581
Y1 - 2014
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1300213
Abstract:
Penicillium digitatum is the most important pathogen of postharvest citrus. gene targeting can be done in P. digitatum using homologous recombination via Agrobacterium tumefaciens mediated transformation (ATMT), but the frequencies are often very low. In the present study, we replaced the Ku80 homolog (a gene of the non-homologous end-joining (NHEJ) pathway) with the hygromycin resistance cassette (hph) by ATMT. No significant change in vegetative growth, conidiation, or pathogenicity was observed in Ku80-deficient strain (ΔPdKu80) of P. digitatum. However, using ΔPdKu80 as a targeting strain, the gene-targeting frequencies for both genes PdbrlA and PdmpkA were significantly increased. These results suggest that Ku80 plays an important role in homologous integration and the created ΔPdKu80 strain would be a good candidate for rapid gene function analysis in P. digitatum.
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CLC number: S436.661.1+1
On-line Access: 2024-08-27
Received: 2023-10-17
Revision Accepted: 2024-05-08
Crosschecked: 2014-01-17
Cited: 3
Clicked: 12967
Open peer comments: Debate/Discuss/Question/Opinion
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Qing-qing Huang<819075736@qq.com>
2015-06-12 10:12:23
Dear author,
Where is the Table S1,I couldn't find it. I want to make it as a reference.I am looking forward to your help