JZUS - Journal of Zhejiang University SCIENCE

Journal of Zhejiang University SCIENCE B 2014 Vol.15 No.2 P.153-164

Cytotoxicity and enhancement activity of essential oil from Zanthoxylum bungeanum Maxim. as a natural transdermal penetration enhancer*

Author(s): Yi Lan¹, Qing Wu¹, Ying-qiu Mao², Qiong Wang¹, Jing An¹, Yan-yan Chen¹, Wen-ping Wang¹, Bo-chen Zhao¹, Na Liu¹, Ye-wen Zhang¹
Affiliation(s): 1. School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China; more
Corresponding email(s): qwu@vip.sina.com
Key Words: Zanthoxylum bungeanum Maxim., Essential oil, Transdermal delivery, Penetration enhancer, HaCaT, Attenuated total reflectance-Fourier transform infrared spectroscopy (ATR-FTIR)

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Yi Lan, Qing Wu, Ying-qiu Mao, Qiong Wang, Jing An, Yan-yan Chen, Wen-ping Wang, Bo-chen Zhao, Na Liu, Ye-wen Zhang. Cytotoxicity and enhancement activity of essential oil from Zanthoxylum bungeanum Maxim. as a natural transdermal penetration enhancer[J]. Journal of Zhejiang University Science B, 2014, 15(2): 153-164.

@article{title="Cytotoxicity and enhancement activity of essential oil from Zanthoxylum bungeanum Maxim. as a natural transdermal penetration enhancer",
author="Yi Lan, Qing Wu, Ying-qiu Mao, Qiong Wang, Jing An, Yan-yan Chen, Wen-ping Wang, Bo-chen Zhao, Na Liu, Ye-wen Zhang",
journal="Journal of Zhejiang University Science B",
volume="15",
number="2",
pages="153-164",
year="2014",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1300230"
}

%0 Journal Article
%T Cytotoxicity and enhancement activity of essential oil from Zanthoxylum bungeanum Maxim. as a natural transdermal penetration enhancer
%A Yi Lan
%A Qing Wu
%A Ying-qiu Mao
%A Qiong Wang
%A Jing An
%A Yan-yan Chen
%A Wen-ping Wang
%A Bo-chen Zhao
%A Na Liu
%A Ye-wen Zhang
%J Journal of Zhejiang University SCIENCE B
%V 15
%N 2
%P 153-164
%@ 1673-1581
%D 2014
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1300230

TY - JOUR
T1 - Cytotoxicity and enhancement activity of essential oil from Zanthoxylum bungeanum Maxim. as a natural transdermal penetration enhancer
A1 - Yi Lan
A1 - Qing Wu
A1 - Ying-qiu Mao
A1 - Qiong Wang
A1 - Jing An
A1 - Yan-yan Chen
A1 - Wen-ping Wang
A1 - Bo-chen Zhao
A1 - Na Liu
A1 - Ye-wen Zhang
J0 - Journal of Zhejiang University Science B
VL - 15
IS - 2
SP - 153
EP - 164
%@ 1673-1581
Y1 - 2014
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1300230

Abstract
Chinese Summary
Academic Network
Reviewer Comment

Abstract: The aim of this present study is to investigate the effect of Zanthoxylum bungeanum oil (essential oil from Z. bungeanum Maxim.) on cytotoxicity and the transdermal permeation of 5-fluorouracil and indomethacin. The cytotoxicity of Z. bungeanum oil on dermal fibroblasts and epidermal keratinocytes was studied using an MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. The rat skin was employed to determine the percutaneous penetration enhancement effect of Z. bungeanum oil on hydrophilic and lipophilic model drugs, i.e., 5-fluorouracil and indomethacin. The secondary structure changes of the rat stratum corneum (SC) were determined using attenuated total reflectance-Fourier transform infrared spectroscopy (ATR-FTIR), and saturated solubilities and SC/vehicle partition coefficients of two model drugs with and without Z. bungeanum oil were also measured to understand its related mechanisms of action. It was found that the half maximal inhibitory concentration (IC₅₀) values of Z. bungeanum oil were significantly lower in haCaT and CCC-ESF-1 cell lines compared to the well-established and standard penetration enhancer Azone. The Z. bungeanum oil at various concentrations effectively facilitated the percutaneous penetration of two model drugs across the rat skin. In addition, the mechanisms of permeation enhancement by Z. bungeanum oil could be explained with saturated solubility, SC/vehicle partition coefficient, and secondary structure changes of SC.

花椒挥发油作为天然经皮促透剂的细胞毒性及促透活性

研究目的：研究花椒挥发油作为天然经皮促透剂的促透活性及其促透机制，同时评价其皮肤细胞毒性。
创新要点：首次评价了花椒挥发油的皮肤细胞毒性、经皮促透活性及其作用机制。
研究方法：利用表皮角质形成细胞（HaCaT）和真皮成纤维细胞（CCC-ESF-1）评价花椒挥发油的细胞毒性；采用亲水性及亲脂性模型药测定挥发油促透活性的基础上研究其促透作用机制。
重要结论：花椒挥发油具有良好促透活性并具有较低皮肤细胞毒性。

关键词：花椒挥发油；天然经皮促透剂；透皮吸收；表皮角质形成细胞（HaCaT）；傅利叶变换红外光谱（ATR-FTIR）

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花椒挥发油作为天然经皮促透剂的细胞毒性及促透活性

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