JZUS - Journal of Zhejiang University SCIENCE

Journal of Zhejiang University SCIENCE B 2014 Vol.15 No.4 P.343-352

Enhanced production of thermostable laccases from a native strain of Pycnoporus sanguineus using central composite design*

Author(s): Leticia I. Ramrez-Cavazos¹, Charles Junghanns², Rakesh Nair³, Diana L. Crdenas-Chvez¹, Carlos Hernndez-Luna⁴, Spiros N. Agathos³, Roberto Parra¹
Affiliation(s): 1. Centro del Agua para Amrica Latina y el Caribe, Tecnolgico de Monterrey, Campus Monterrey, NL 64849, Mexico; more
Corresponding email(s): r.parra@itesm.mx
Key Words: Enhanced laccase production, Central composite design, Screening media, Inducers, Tomato juice medium, Soybean oil, Copper sulfate

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Leticia I. Ramírez-Cavazos, Charles Junghanns, Rakesh Nair, Diana L. Cárdenas-Chávez, Carlos Hernández-Luna, Spiros N. Agathos, Roberto Parra. Enhanced production of thermostable laccases from a native strain of Pycnoporus sanguineus using central composite design[J]. Journal of Zhejiang University Science B, 2014, 15(4): 343-352.

@article{title="Enhanced production of thermostable laccases from a native strain of Pycnoporus sanguineus using central composite design",
author="Leticia I. Ramírez-Cavazos, Charles Junghanns, Rakesh Nair, Diana L. Cárdenas-Chávez, Carlos Hernández-Luna, Spiros N. Agathos, Roberto Parra",
journal="Journal of Zhejiang University Science B",
volume="15",
number="4",
pages="343-352",
year="2014",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1300246"
}

%0 Journal Article
%T Enhanced production of thermostable laccases from a native strain of Pycnoporus sanguineus using central composite design
%A Leticia I. Ramírez-Cavazos
%A Charles Junghanns
%A Rakesh Nair
%A Diana L. Cárdenas-Chávez
%A Carlos Hernández-Luna
%A Spiros N. Agathos
%A Roberto Parra
%J Journal of Zhejiang University SCIENCE B
%V 15
%N 4
%P 343-352
%@ 1673-1581
%D 2014
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1300246

TY - JOUR
T1 - Enhanced production of thermostable laccases from a native strain of Pycnoporus sanguineus using central composite design
A1 - Leticia I. Ramírez-Cavazos
A1 - Charles Junghanns
A1 - Rakesh Nair
A1 - Diana L. Cárdenas-Chávez
A1 - Carlos Hernández-Luna
A1 - Spiros N. Agathos
A1 - Roberto Parra
J0 - Journal of Zhejiang University Science B
VL - 15
IS - 4
SP - 343
EP - 352
%@ 1673-1581
Y1 - 2014
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1300246

Abstract
Chinese Summary
Academic Network
Reviewer Comment

Abstract: The production of thermostable laccases from a native strain of the white-rot fungus Pycnoporus sanguineus isolated in Mexico was enhanced by testing different media and a combination of inducers including copper sulfate (CuSO₄). The best conditions obtained from screening experiments in shaken flasks using tomato juice, CuSO₄, and soybean oil were integrated in an experimental design. Enhanced levels of tomato juice as the medium, CuSO₄ and soybean oil as inducers (36.8% (v/v), 3 mmol/L, and 1% (v/v), respectively) were determined for 10 L stirred tank bioreactor runs. This combination resulted in laccase titer of 143 000 IU/L (2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid), pH 3.0), which represents the highest activity so far reported for P. sanguineus in a 10-L fermentor. Other interesting media resulting from the screening included glucose-bactopeptone which increased laccase activity up to 20 000 IU/L, whereas the inducers Acid Blue 62 and Reactive Blue 19 enhanced enzyme production in this medium 10 times. Based on a partial characterization, the laccases of this strain are especially promising in terms of thermostability (half-life of 6.1 h at 60 °C) and activity titers.

中心组合设计方法提高血红密孔菌（Pycnoporus sanguineus）耐热漆酶的产量

研究目的：优化获得血红密孔菌（P. sanguineus）的最佳培养基组成，提高耐热漆酶的产量。
创新要点：获得了目前文献报道的最高水平的漆酶活力。
研究方法：通过单因素试验研究了不同培养基（番茄汁、麦麸、麦芽提取物和葡萄糖细菌蛋白胨培养基）和不同组合诱导剂（大豆油、阿魏酸、没食子酸、二甲基苯胺、酸性蓝62和活性蓝19分别与硫酸铜组合诱导剂）对P. sanguineus产耐热漆酶的影响。在此基础上采用中心组合试验设计，进一步研究了番茄汁培养基结合硫酸铜和大豆油组合诱导剂对P. sanguineus产耐热漆酶的影响。利用SAS10.0和响应面分析方法对试验结果进行了统计分析和建立回归模型。
重要结论：通过中心组合设计优化得出P. sanguineus产耐热漆酶的最优培养基条件：以36.8%番茄汁为培养基，以3 mmol/L硫酸铜和1%大豆油作为组合诱导剂。该条件下在10 L搅拌槽生物反应器中漆酶活力达到了143000 IU/L（2,2'-联氮双(3-乙基苯并噻唑啉-6-磺酸)为底物，pH值为3.0）。

关键词：漆酶产量；中心组合设计；培养基筛选；诱导剂；番茄汁；大豆油；硫酸铜

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References

[1] Baldrian, P., 2003. Interactions of heavy metals with white-rot fungi. Enzyme Microb Technol, 32(1):78-91.

[2] Bertrand, T., Jolivalt, C., Briozzo, P., 2002. Crystal structure of a four-copper laccase complexed with an arylamine: insights into substrate recognition and correlation with kinetics. Biochemistry, 41(23):7325-7333.

[3] Bollag, J.M., Leonowicz, A., 1984. Comparative studies of extracellular fungal laccases. Appl Environ Microbiol, 48(4):849-854.

[4] Carbajo, J., Junca, H., Terrn, M., 2002. Tannic acid induces transcription of laccase gene cglcc1 in the white-rot fungus Coriolopsis gallica . Can J Microbiol, 48(12):1041-1047.

[5] Chu, I.M., Lee, C., Li, T.S., 1992. Production and degradation of alkaline protease in batch cultures of Bacillus subtilis ATCC 14416. Enzyme Microb Technol, 14(9):755-761.

[6] Collins, P.J., Dobson, A.D., 1997. Regulation of laccase gene transcription in Trametes versicolor . Appl Environ Microbiol, 63(9):3444-3450.

[7] Dantn-Gonzlez, E., Vite-Vallejo, O., Martnez-Anaya, C., 2008. Production of two novel laccase isoforms by a thermotolerant strain of Pycnoporus sanguineus isolated from an oil-polluted tropical habitat. Int Microbiol, 11:163-169.

[8] Dekker, R.F., Barbosa, A.M., Giese, E.C., 2007. Influence of nutrients on enhancing laccase production by Botryosphaeria rhodina MAMB-05. Int Microbiol, 10(3):177-185.

[9] Eggert, C., Temp, U., Erikkson, K.E.L., 1996. The ligninolytic system of the white-rot fungus Pycnoporus cinnabarinus: purification and characterization of the laccase. Appl Environ Microbiol, 62(4):1151-1158.

[10] Galhaup, C., Haltrich, D., 2001. Enhanced formation of laccase activity by the white rot fungus Trametes pubescens in the presence of copper. Appl Microbiol Biotechnol, 56(1-2):225-232.

[11] Galhaup, C., Wagner, H., Hinterstoisser, B., 2002. Increased production of laccase by the wood-degrading basidiomycete Trametes pubescens . Enzyme Microb Technol, 30(4):529-536.

[12] Gochev, V.K., Krastanov, A.I., 2007. Fungal laccases. Bulg J Agric Sci, 13:75-83.

[13] Hernndez-Luna, C., Gutirrez-Soto, G., Salcedo-Martinez, S., 2008. Screening for decolorizing basidiomycetes in Mexico. W J Microbiol Biotechnol, 24(4):465-473.

[14] Junghanns, C., Parra, R., Keshavarz, T., 2008. Towards higher laccase activities produced by aquatic ascomycetous fungi through combination of elicitors and an alternative substrate. Eng Life Sci, 8(3):277-285.

[15] Kudanga, T., Nyanhongo, G.S., Guebitz, G.M., 2011. Potential applications of laccase-mediated coupling and grafting reactions: a review. Enzyme Microb Technol, 48(3):195-208.

[16] Kunamneni, A., Ballesteros, A., Plou, F.J., 2007. Fungal laccase—a versatile enzyme for biotechnological applications. Communicating Current Research and Educational Topics and Trends in Applied Microbiology. Formatex-Badajoz,Spain :233-244.

[17] Lee, I.Y., Jung, K.H., Lee, C.H., 1999. Enhanced production of laccase in Trametes versicolor by the addition of ethanol. Biotechnol Lett, 21(11):965-968.

[18] Leonowicz, A., Trojanowski, J., Orlicz, B., 1978. Induction of laccase in Basidiomycetes: apparent activity of the inducible and constitutive forms of the enzyme with phenolic substrates. Acta Biochim Pol, 25(4):369-378.

[19] Liers, C., Ullrich, R., Pecyna, M., 2007. Production, purification and partial enzymatic and molecular characterization of a laccase from the wood-rotting ascomycete Xylaria polymorpha . Enzyme Microb Technol, 41(6-7):785-793.

[20] Litthauer, D., van Vuuren, M.J., van Tonder, A., 2007. Purification and kinetics of a thermostable laccase from Pycnoporus sanguineus (SCC 108). Enzyme Microb Technol, 40(4):563-568.

[21] Liu, L., Lin, Z., Zheng, T., 2009. Fermentation optimization and characterization of the laccase from Plerotus ostreatus strain 10969. Enzyme Microb Technol, 44(6-7):426-433.

[22] Lomascolo, A., Cayol, J.L., Roche, M., 2002. Molecular clustering of Pycnoporus strains from various geographic origins and isolation of monokariotic strains for laccase hyperproduction. Mycol Res, 106(10):1193-1203.

[23] Michniewicz, A., Ullrich, R., Ledakowicz, S., 2006. The white-rot fungus Cerrena unicolor strain 137 produces two laccase isoforms with different physico-chemical and catalytic properties. Appl Microbiol Biotechnol, 69(6):682-688.

[24] Mueangtoom, K., Kittl, R., Mann, O., 2010. Low pH dye decolorization with ascomycete Lamprospora wrightii laccase. Biotechnol J, 5(8):857-870.

[25] Nair, R.R., Demarche, P., Agathos, S.N., 2013. Formulation and characterization of an immobilized laccase biocatalyst and its application to eliminate organic micropollutants in wastewater. N Biotechnol, 30(6):814-823.

[26] Osma, J.F., Saravia, V., Toca-Herrera, J., 2007. Mandarin peelings: the best carbon source to produce laccase by static cultures of Trametes pubescens . Chemosphere, 67(8):1677-1680.

[27] Osma, J.F., Toca-Herrera, J.L., Rodrguez-Couto, S., 2011. Cost analysis in laccase production. J Environ Manage, 92(11):2907-2912.

[28] Palmieri, G., Giardina, P., Bianco, C., 2000. Copper induction of laccase isoenzymes in the ligninolytic fungus Pleurotus ostreatus . Appl Environ Microbiol, 66(3):920-924.

[29] Pointing, S.B., Jones, E., Vrijmoed, L., 2000. Optimization of laccase production by Pycnoporus sanguineus in submerged liquid culture. Mycologia, 92(1):139-144.

[30] Rodrguez-Couto, S., Toca-Herrera, J.L., 2007. Laccase production at reactor scale by filamentous fungi. Biotechnol Adv, 25(6):558-569.

[31] Rodrguez-Couto, S., Osma, J.F., Toca-Herrera, J.L., 2009. Removal of synthetic dyes by an eco-friendly strategy. Eng Life Sci, 9(2):116-123.

[32] Rogalski, J., Janusz, G., Legiec, D., 2011. Purification of extracellular laccase from Rhizoctonia praticola . J Fac Agric, 56(1):1-7.

[33] Trovaslet, M., Estelle, E., Guiavarch, Y., 2007. Potential of a Pycnoporus sanguineus laccase in bioremediation of wastewater and kinetic activation in the presence of an anthraquinonic acid dye. Enzyme Microb Technol, 41(3):368-376.

[34] Ullrich, R., Huong, L.M., Dung, N.L., 2005. Laccase from the medicinal mushroom Agaricus blazei: production, purification and characterization. Appl Microbiol Biotechnol, 67(3):357-363.

[35] Uzan, E., Nousiainen, P., Balland, V., 2010. High redox potential laccases from the ligninolytic fungi Pycnoporus coccineus and Pycnoporus sanguineus suitable for white biotechnology: from gene cloning to enzyme characterization and applications. J Appl Microbiol, 108(6):2199-2213.

[36] van der Merwe, J.J., 2002. Production of Laccase by the White-Rot Fungus . MS Thesis, University of the Free State,Bloemfontein, South Africa :

[37] Vanhulle, S., Radman, R., Parra, R., 2007. Effect of mannan oligosaccharide elicitor and ferulic acid on enhancement of laccases. Enzyme Microb Technol, 40(7):1712-1718.

[38] Vanhulle, S., Enaud, E., Trovaslet, M., 2007. Overlap of laccases/cellobiose dehydrogenase activities during the decolourisation of anthraquinonic dyes with close chemical structures by Pycnoporus strains. Enzyme Microb Technol, 40(7):1723-1731.

[39] Vite-Vallejo, O., Palomares, L.A., Dantn-Gonzlez, E., 2009. The role of N-glycosylation on the enzymatic activity of a Pycnoporus sanguineus laccase. Enzyme Microb Technol, 45(3):233-239.

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中心组合设计方法提高血红密孔菌（Pycnoporus sanguineus）耐热漆酶的产量

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References