JZUS - Journal of Zhejiang University SCIENCE

Journal of Zhejiang University SCIENCE B 2014 Vol.15 No.6 P.540-547

Ferulic acid prevents liver injury and increases the anti-tumor effect of diosbulbin B in vivo *

Author(s): Jun-ming Wang^1,3, Yu-chen Sheng², Li-li Ji¹, Zheng-tao Wang¹
Affiliation(s): 1. 1. MOE Key Laboratory for Standardization of Chinese Medicines and Shanghai Key Laboratory of Complex Prescription, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China more
Corresponding email(s): lichenyue1307@126.com
Key Words: Ferulic acid, Diosbulbin B, Hepatotoxicity, Oxidative stress injury, Anti-tumor activity

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Jun-ming Wang, Yu-chen Sheng, Li-li Ji, Zheng-tao Wang. Ferulic acid prevents liver injury and increases the anti-tumor effect of diosbulbin B in vivo[J]. Journal of Zhejiang University Science B, 2014, 15(6): 540-547.

@article{title="Ferulic acid prevents liver injury and increases the anti-tumor effect of diosbulbin B in vivo",
author="Jun-ming Wang, Yu-chen Sheng, Li-li Ji, Zheng-tao Wang",
journal="Journal of Zhejiang University Science B",
volume="15",
number="6",
pages="540-547",
year="2014",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1300250"
}

%0 Journal Article
%T Ferulic acid prevents liver injury and increases the anti-tumor effect of diosbulbin B in vivo
%A Jun-ming Wang
%A Yu-chen Sheng
%A Li-li Ji
%A Zheng-tao Wang
%J Journal of Zhejiang University SCIENCE B
%V 15
%N 6
%P 540-547
%@ 1673-1581
%D 2014
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1300250

TY - JOUR
T1 - Ferulic acid prevents liver injury and increases the anti-tumor effect of diosbulbin B in vivo
A1 - Jun-ming Wang
A1 - Yu-chen Sheng
A1 - Li-li Ji
A1 - Zheng-tao Wang
J0 - Journal of Zhejiang University Science B
VL - 15
IS - 6
SP - 540
EP - 547
%@ 1673-1581
Y1 - 2014
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1300250

Abstract
Chinese Summary
Academic Network
Reviewer Comment

Abstract: The present study is designed to investigate the protection by ferulic acid against the hepatotoxicity induced by diosbulbin B and its possible mechanism, and further observe whether ferulic acid augments diosbulbin B-induced anti-tumor activity. The results show that ferulic acid decreases diosbulbin B-increased serum alanine transaminase/aspartate transaminase (ALT/AST) levels. ferulic acid also decreases lipid peroxide (LPO) levels which are elevated in diosbulbin B-treated mice. Histological evaluation of the liver demonstrates hydropic degeneration in diosbulbin B-treated mice, while ferulic acid reverses this injury. Moreover, the activities of copper- and zinc-containing superoxide dismutase (CuZn-SOD) and catalase (CAT) are decreased in the livers of diosbulbin B-treated mice, while ferulic acid reverses these decreases. Further results demonstrate that the mRNA expressions of CuZn-SOD and CAT in diosbulbin B-treated mouse liver are significantly decreased, while ferulic acid prevents this decrease. In addition, ferulic acid also augments diosbulbin B-induced tumor growth inhibition compared with diosbulbin B alone. Taken together, the present study shows that ferulic acid prevents diosbulbin B-induced liver injury via ameliorating diosbulbin B-induced liver oxidative stress injury and augments diosbulbin B-induced anti-tumor activity.

阿魏酸降低黄独素B肝毒性并提高其抗肿瘤活性

研究目的：观察阿魏酸对黄独素B诱导肝毒性的抑制活性及其机理，同时探索阿魏酸对黄独素B抗肿瘤活性的增效作用。
创新要点：黄独素B为中药黄药子抗肿瘤的主要药效活性成分，但同时又是其致肝毒性的主要毒性成分。本研究立足于中医药配伍减毒增效理论，试图通过试验考察配伍阿魏酸对黄独素B肝毒性/抗肿瘤活性的减毒增效作用，为黄独素B与阿魏酸联合应用于抗肿瘤提供了一定的临床前试验依据。
研究方法：荷瘤小鼠（S180肉瘤）连续12天灌胃给药阿魏酸和黄独素B。通过对血清丙氨酸/天冬氨酸转氨酶活性（见图2）、肝脂质过氧化（见图3）和肝组织病理分析（见图4）考察阿魏酸对黄独素B肝毒性的抑制作用；通过对铜锌-超氧化物歧化酶和过氧化氢酶的活性（见图5a、5b）和基因表达分析（见图5c）探讨阿魏酸抑制黄独素B肝毒性的机理；通过对瘤重、抑瘤率的统计分析阿魏酸增加的黄独素B抗肿瘤活性。
重要结论：阿魏酸可以通过改善黄独素B诱导的氧应激损伤从而抑制其肝毒性，同时还可以协同增加黄独素B的抗肿瘤活性。

关键词：阿魏酸；黄独素B；肝毒性；氧应激损伤；抗肿瘤

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阿魏酸降低黄独素B肝毒性并提高其抗肿瘤活性

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