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Abstract: Agriculture is the foundation of social development. Under the pressure of population growth, natural disasters, environmental pollution, climate change, and food safety, the interdisciplinary “new agriculture” is becoming an important trend of modern agriculture. In fact, new agriculture is not only the foundation of great health and new energy sources, but is also the cornerstone of national food security, energy security, and biosafety. hydrogen agronomy focuses mainly on the mechanism of hydrogen gas (H₂) biology effects in agriculture, and provides a theoretical foundation for the practice of hydrogen agriculture, a component of the new agriculture. Previous research on the biological effects of H₂ focused chiefly on medicine. The mechanism of selective antioxidant is the main theoretical basis of hydrogen medicine. Subsequent experiments have demonstrated that H₂ can regulate the growth and development of plant crops, edible fungus, and livestock, and enhance the tolerance of these agriculturally important organisms against abiotic and biotic stresses. Even more importantly, H₂ can regulate the growth and development of crops by changing the soil microbial community composition and structure. Use of H₂ can also improve the nutritional value and postharvest quality of agricultural products. Researchers have also shown that the biological functions of molecular hydrogen are mediated by modulating reactive oxygen species (ROS), nitric oxide (NO), and carbon monoxide (CO) signaling cascades in plants and microbes. This review summarizes and clarifies the history of hydrogen agronomy and describes recent progress in the field. We also argue that emerging hydrogen agriculture will be an important direction in the new agriculture. Further, we discuss several scientific problems in hydrogen agronomy, and suggest that the future of hydrogen agronomy depends on contributions by multiple disciplines. Important future research directions of hydrogen agronomy include hydrogen agriculture in special environments, such as islands, reefs, aircraft, and outer space.

氢农学:研究进展与展望

[1]Angus JF, Kirkegaard JA, Hunt JR, et al., 2015. Break crops and rotations for wheat. Crop Pasture Sci, 66(6):523-552.

[2]Árnason B, Sigfússon TI, 2000. Iceland—a future hydrogen economy. Int J Hydrogen Energy, 25(5):389-394.

[3]Cao ZY, Duan XL, Yao P, et al., 2017. Hydrogen gas is involved in auxin-induced lateral root formation by modulating nitric oxide synthesis. Int J Mol Sci, 18(10):2084.

[4]Chen M, Cui WT, Zhu KK, et al., 2014. Hydrogen-rich water alleviates aluminum-induced inhibition of root elongation in alfalfa via decreasing nitric oxide production. J Hazard Mater, 267:40-47.

[5]Chen Y, Wang M, Hu LL, et al., 2017. Carbon monoxide is involved in hydrogen gas-induced adventitious root development in cucumber under simulated drought stress. Front Plant Sci, 8:128.

[6]Constant P, Poissant L, Villemur R, 2009. Tropospheric H₂ budget and the response of its soil uptake under the changing environment. Sci Total Environ, 407(6):1809-1823.

[7]Cui WT, Gao CY, Fang P, et al., 2013. Alleviation of cadmium toxicity in Medicago sativa by hydrogen-rich water. J Hazard Mater, 260:715-724.

[8]Cui WT, Fang P, Zhu KK, et al., 2014. Hydrogen-rich water confers plant tolerance to mercury toxicity in alfalfa seedlings. Ecotoxicol Environ Saf, 105:103-111.

[9]Cui WT, Yao P, Pan JC, et al., 2020. Transcriptome analysis reveals insight into molecular hydrogen-induced cadmium tolerance in alfalfa: the prominent role of sulfur and (homo)glutathione metabolism. BMC Plant Biol, 20:58.

[10]Czerkawski JW, 1972. Fate of metabolic hydrogen in the rumen. Proc Nutr Soc, 31(2):141-146.

[11]http://doi.org/10.1079/PNS19720028

[12]Dai C, Cui WT, Pan JC, et al., 2017. Proteomic analysis provides insights into the molecular bases of hydrogen gas-induced cadmium resistance in Medicago sativa. J Proteomics, 152:109-120.

[13]Das D, Khanna N, Veziroğlu NT, 2008. Recent developments in biological hydrogen production processes. Chem Ind Chem Eng Quart, 14(2):57-67.

[14]Derwent RG, Collins WJ, Johnson CE, et al., 2001. Transient behaviour of tropospheric ozone precursors in a global 3-D CTM and their indirect greenhouse effects. Clim Change, 49(4):463-487.

[15]Dole M, Wilson FR, Fife WP, 1975. Hyperbaric hydrogen therapy: a possible treatment for cancer. Science, 190(4210):152-154.

[16]http://doi.org/10.1126/science.1166304

[17]Dong Z, Layzell DB, 2001. H₂ oxidation, O₂ uptake and CO₂ fixation in hydrogen treated soils. Plant Soil, 229:1-12.

[18]Dong Z, Wu L, Kettlewell B, et al., 2003. Hydrogen fertilization of soils—is this a benefit of legumes in rotation? Plant Cell Environ, 26(11):1875-1879.

[19]Dresselhaus MS, Thomas IL, 2001. Alternative energy technologies. Nature, 414(6861):332-337.

[20]Du ZM, Jia HP, Liu J, et al., 2014. Protective effects of hydrogen-rich saline in uncontrolled hemorrhagic shock. Exp Ther Med, 7(5):1253-1258.

[21]Fu Y, Ito M, Fujita Y, et al., 2009. Molecular hydrogen is protective against 6-hydroxydopamine-induced nigrostriatal degeneration in a rat model of Parkinson’s disease. Neurosci Lett, 453(2):81-85.

[22]Gaffron H, 1939. Reduction of carbon dioxide with molecular hydrogen in green algae. Nature, 143(3614):204-205.

[23]Ge L, Yang M, Yang NN, et al., 2017. Molecular hydrogen: a preventive and therapeutic medical gas for various diseases. Oncotarget, 8(60):102653-102673.

[24]https://doi.org/10.18632/oncotarget.21130

[25]Gest H, Kamen MD, 1949. Studies on the metabolism of photosynthetic bacteria IV: photochemical production of molecular hydrogen by growing cultures of photosynthetic bacteria. J Bacteriol, 58(2):239-245.

[26]http://doi.org/10.1128/JB.58.2.239-245.1949

[27]Golding AL, Dong ZM, 2010. Hydrogen production by nitrogenase as a potential crop rotation benefit. Environ Chem Lett, 8(2):101-121.

[28]Grassi D, Desideri G, Ferri L, et al., 2010. Oxidative stress and endothelial dysfunction: say NO to cigarette smoking! Curr Pharm Design, 16(23):2539-2550.

[29]Guan Q, Ding XW, Jiang R, et al., 2019. Effects of hydrogen-rich water on the nutrient composition and antioxidative characteristics of sprouted black barley. Food Chem, 299: 125095.

[30]Guo SX, Fang Q, You CG, et al., 2015. Effects of hydrogen-rich saline on early acute kidney injury in severely burned rats by suppressing oxidative stress induced apoptosis and inflammation. J Transl Med, 13:183.

[31]Hou C, Peng YH, Qin C, et al., 2018. Hydrogen-rich water improves cognitive impairment gender-dependently in APP/PS1 mice without affecting Aβ clearance. Free Radic Res, 52(11-12):1311-1322.

[32]Hu HL, Li PX, Wang YN, et al., 2014. Hydrogen-rich water delays postharvest ripening and senescence of kiwifruit. Food Chem, 156:100-109.

[33]Hu HL, Zhao SP, Li PX, et al., 2018. Hydrogen gas prolongs the shelf life of kiwifruit by decreasing ethylene biosynthesis. Postharvest Biol Tech, 135:123-130.

[34]Huang DJ, Bian BT, Zhang ML, et al., 2020. The role and proteomic analysis of ethylene in hydrogen gas-induced adventitious rooting development in cucumber (Cucumis sativus L.) explants. PeerJ, 8(15):e8896.

[35]http://doi.org/10.7717/peerj.8896

[36]Ichimasa M, Ichimasa Y, Yagi Y, et al., 1989. Oxidation of atmospheric molecular tritium in plant leaves, lichens and mosses. J Radiat Res, 30(4):323-329.

[37]Ji X, Zhang Q, Zheng WJ, et al., 2019. Morphological and molecular response of small intestine to lactulose and hydrogen-rich water in female piglets fed Fusarium mycotoxins contaminated diet. J Anim Sci Biotechnol, 10:9.

[38]Jin QJ, Zhu KK, Cui WT, et al., 2013. Hydrogen gas acts as a novel bioactive molecule in enhancing plant tolerance to paraquat-induced oxidative stress via the modulation of heme oxygenase-1 signalling system. Plant Cell Environ, 36(5):956-969.

[39]Jin QJ, Cui WT, Dai C, et al., 2016. Involvement of hydrogen peroxide and heme oxygenase-1 in hydrogen gas-induced osmotic stress tolerance in alfalfa. Plant Growth Regul, 80(2):215-223.

[40]Kajiyama S, Hasegawa G, Asano M, et al., 2008. Supplementation of hydrogen-rich water improves lipid and glucose metabolism in patients with type 2 diabetes or impaired glucose tolerance. Nutr Res, 28(3):137-143.

[41]Kamimura N, Ichimiya H, Iuchi K, et al., 2016. Molecular hydrogen stimulates the gene expression of transcriptional coactivator PGC-1α to enhance fatty acid metabolism. NPJ Aging Mech Dis, 2:16008.

[42]Kang KM, Kang YN, Choi IB, et al., 2011. Effects of drinking hydrogen-rich water on the quality of life of patients treated with radiotherapy for liver tumors. Med Gas Res, 1:11.

[43]Kawamura T, Wakabayashi N, Shigemura N, et al., 2013. Hydrogen gas reduces hyperoxic lung injury via the Nrf2 pathway in vivo. Am J Physiol Lung Cell Mol Physiol, 304(10):L646-L656.

[44]Kim YW, Byzova TV, 2014. Oxidative stress in angiogenesis and vascular disease. Blood, 123(5):625-631.

[45]Kirkegaard J, Christen O, Krupinsky J, et al., 2008. Break crop benefits in temperate wheat production. Field Crops Res, 107(3):185-195.

[46]Kou Z, Zhao PH, Wang ZH, et al., 2019. Acid-responsive H₂-releasing Fe nanoparticles for safe and effective cancer therapy. J Mater Chem B, 7(17):2759-2765.

[47]Lesk C, Rowhani P, Ramankutty N, 2016. Influence of extreme weather disasters on global crop production. Nature, 529(7584):84-87.

[48]Lewandrowski J, Tobey J, Cook Z, 1997. The interface between agricultural assistance and the environment: chemical fertilizer consumption and area expansion. Land Econ, 73(3):404-427.

[49]Li CX, Gong TY, Bian BT, et al., 2018. Roles of hydrogen gas in plants: a review. Funct Plant Biol, 45(8):783-792.

[50]Lin YT, Zhang W, Qi F, et al., 2014. Hydrogen-rich water regulates cucumber adventitious root development in a heme oxygenase-1/carbon monoxide-dependent manner. J Plant Physiol, 171(2):1-8.

[51]Liu JK, Wang XY, Shigenaga MK, et al., 1996. Immobilization stress causes oxidative damage to lipid, protein, and DNA in the brain of rats. FASEB J, 10(13):1532-1538.

[52]Liu SC, Donahue TM, 1974. The aeronomy of hydrogen in the atmosphere of the earth. J Atoms Sci, 31(4):1118-1136.

[53]Liu YQ, Liu YF, Ma XM, et al., 2015. Hydrogen-rich saline attenuates skin ischemia/reperfusion induced apoptosis via regulating Bax/Bcl-2 ratio and ASK-1/JNK pathway. J Plast Reconstr Aesthet Surg, 68(7):e147-e156.

[54]Long P, Yan WM, He MS, et al., 2019. Protective effects of hydrogen gas in a rat model of branch retinal vein occlusion via decreasing VEGF-α expression. BMC Ophthalmol, 19:112.

[55]Lowry GV, Avellan A, Gilbertson LM, 2019. Opportunities and challenges for nanotechnology in the agri-tech revolution. Nat Nanotechnol, 14(6):517-522.

[56]Lu H, Wu BQ, Wang YF, et al., 2017. Effects of hydrogen-rich water treatment on defense responses of postharvest tomato fruit to Botrytis cinerea. J Henan Agric Sci, 46(2):64-68 (in Chinese).

[57]https://doi.org/10.15933/j.cnki.1004-3268.2017.02.013

[58]Lu HT, Sun XJ, 2018. Hydrogen medicine: research advance, controversy and challenges. Acad J Second Mil Med Univ, 39(11):1181-1187 (in Chinese).

[59]https://doi.org/10.16781/j.0258-879x.2018.11.1181

[60]Lubitz W, Ogata H, Rüdiger O, et al., 2014. Hydrogenases. Chem Rev, 114(8):4081-4148.

[61]Lytos A, Lagkas T, Sarigiannidis P, et al., 2020. Towards smart farming: systems, frameworks and exploitation of multiple sources. Comput Netw, 172:107147.

[62]Ma XM, Zhang X, Xie F, et al., 2020. Bio-enzyme basis of hydrogen in biological system. Curr Biotech, 10(1):15-22 (in Chinese).

[63]https://doi.org/10.19586/j.2095-2341.2020.0002

[64]Macrae RJ, Henning J, Hill SB, 1993. Strategies to overcome barriers to the development of sustainable agriculture in Canada: the role of agribusiness. J Agric Environ Ethics, 6(1):21-51.

[65]Maeder P, Fliessbach A, Dubois D, et al., 2002. Soil fertility and biodiversity in organic farming. Science, 296(5573):1694-1697.

[66]McLearn N, Dong ZM, 2002. Microbial nature of the hydrogen-oxidizing agent in hydrogen-treated soil. Biol Fertil Soils, 35(6):465-469.

[67]Melis A, Zhang LP, Forestier M, et al., 2000. Sustained photobiological hydrogen gas production upon reversible inactivation of oxygen evolution in the green alga Chlamydomonas reinhardtii. Plant Physiol, 122(1):127-136.

[68]Melman DA, Kelly C, Schneekloth J, et al., 2019. Tillage and residue management drive rapid changes in soil macrofauna communities and soil properties in a semiarid cropping system of Eastern Colorado. Appl Soil Ecol, 143:98-106.

[69]Mijangos I, Pérez R, Albizu I, et al., 2006. Effects of fertilization and tillage on soil biological parameters. Enzyme Microb Technol, 40(1):100-106.

[70]Nicolopoulou-Stamati P, Maipas S, Kotampasi C, et al., 2016. Chemical pesticides and human health: the urgent need for a new concept in agriculture. Front Public Health, 4:148.

[71]Nishimaki K, Asada T, Ohsawa I, et al., 2018. Effects of molecular hydrogen assessed by an animal model and a randomized clinical study on mild cognitive impairment. Curr Alzheimer Res, 15(5):482-492.

[72]Novelli PC, Lang PM, Masarie KA, et al., 1999. Molecular hydrogen in the troposphere: global distribution and budget. J Geophys Res Atmos, 104(D23):30427-30444.

[73]Ohsawa I, Ishikawa M, Takahashi K, et al., 2007. Hydrogen acts as a therapeutic antioxidant by selectively reducing cytotoxic oxygen radicals. Nat Med, 13(6):688-694.

[74]Ohta S, 2011. Recent progress toward hydrogen medicine: potential of molecular hydrogen for preventive and therapeutic applications. Curr Pharm Design, 17(22):2241-2252.

[75]Ohta S, 2014. Molecular hydrogen as a preventive and therapeutic medical gas: initiation, development and potential of hydrogen medicine. Pharmacol Therapeut, 144(1):1-11.

[76]Paneth FA, 1937. The chemical composition of the atmosphere. Quart J Roy Meteorol Soc, 63(271):433-443.

[77]Pimentel D, 1996. Green revolution agriculture and chemical hazards. Sci Total Environ, 188:S86-S98.

[78]Qaswar M, Jing H, Ahmed W, et al., 2020. Yield sustainability, soil organic carbon sequestration and nutrients balance under long-term combined application of manure and inorganic fertilizers in acidic paddy soil. Soil Tillage Res, 198:104569.

[79]Qi LS, Yao L, Liu W, et al., 2015. Sirtuin type 1 mediates the retinal protective effect of hydrogen-rich saline against light-induced damage in rats. Invest Ophth Vis Sci, 56(13):8268-8279.

[80]Ren A, Liu R, Miao ZG, et al., 2017. Hydrogen-rich water regulates effects of ROS balance on morphology, growth and secondary metabolism via glutathione peroxidase in Ganoderma lucidum. Environ Microbiol, 19(2):566-583.

[81]Ren PJ, Jin X, Liao WB, et al., 2017. Effect of hydrogen-rich water on vase life and quality in cut lily and rose flowers. Hortic Environ Biotechnol, 58(6):576-584.

[82]Renwick GM, Giumarro C, Siegel SM, 1964. Hydrogen metabolism in higher plants. Plant Physiol, 39(3):303-306.

[83]http://doi.org/10.1104/pp.39.3.303

[84]Runtuwene J, Amitani H, Amitani M, et al., 2015. Hydrogen-water enhances 5-fluorouracil-induced inhibition of colon cancer. PeerJ, 3:e859.

[85]Saitoh Y, Okayasu H, Xiao L, et al., 2008. Neutral pH hydrogen-enriched electrolyzed water achieves tumor-preferential clonal growth inhibition over normal cells and tumor invasion inhibition concurrently with intracellular oxidant repression. Oncol Res, 17(6):247-255.

[86]Sauer H, Wartenberg M, Hescheler J, 2001. Reactive oxygen species as intracellular messengers during cell growth and differentiation. Cell Physiol Biochem, 11(4):173-186.

[87]Schubert KR, Evans HJ, 1976. Hydrogen evolution: a major factor affecting the efficiency of nitrogen fixation in nodulated symbionts. Proc Natl Acad Sci USA, 73(4):1207-1211.

[88]Shen WB, Sun XJ, 2019. Hydrogen biology: it is just beginning. Chin J Biochem Mol Biol, 35(10):1037-1050 (in Chinese).

[89]https://doi.org/10.13865/j.cnki.cjbmb.2019.10.01

[90]Shen WB, Xie YJ, Cao ZY, et al., 2014. Hydrogen-rich Liquid Plant Growth Regulator: Preparation and Application. CN201210154005.0 (in Chinese).

[91]Shen WB, Zhang YH, Su JC, et al., 2020. Hydrogen-rich Degradation Agent Capable of Reducing Nitrite Accumulation in Post-harvest Vegetables and Fruits: Preparation and Application. CN201710124039.8 (in Chinese).

[92]Shen WB, Su JC, Sun XJ, 2018. Research progress in the botanical effects of hydrogen gas. J Nanjing Agric Univ, 41(3):392-401 (in Chinese).

[93]Song GH, Tian H, Qin SC, et al., 2012. Hydrogen decreases athero-susceptibility in apolipoprotein B-containing lipoproteins and aorta of apolipoprotein E knockout mice. Atherosclerosis, 221(1):55-65.

[94]Stein S, Selesi D, Schilling R, et al., 2005. Microbial activity and bacterial composition of H₂-treated soils with net CO₂ fixation. Soil Biol Biochem, 37(10):1938-1945.

[95]Stephenson M, Stickland LH, 1931. Hydrogenase: a bacterial enzyme activating molecular hydrogen: the properties of the enzyme. Biochem J, 25(1):205-214.

[96]Su JC, Zhang YH, Nie Y, et al., 2018. Hydrogen-induced osmotic tolerance is associated with nitric oxide-mediated proline accumulation and reestablishment of redox balance in alfalfa seedlings. Environ Exp Bot, 147:249-260.

[97]Su JC, Nie Y, Zhao G, et al., 2019. Endogenous hydrogen gas delays petal senescence and extends the vase life of lisianthus cut flowers. Postharvest Biol Technol, 147: 148-155.

[98]Su NN, Wu Q, Liu YY, et al., 2014. Hydrogen-rich water reestablishes ROS homeostasis but exerts differential effects on anthocyanin synthesis in two varieties of radish sprouts under UV-A irradiation. J Agric Food Chem, 62(27):6454-6462.

[99]Tanriverdi H, Evrengul H, Kuru O, et al., 2006. Cigarette smoking induced oxidative stress may impair endothelial function and coronary blood flow in angiographically normal coronary arteries. Circ J, 70(5):593-599.

[100]Tian L, Zhang L, Xia F, et al., 2013. Hydrogen-rich saline ameliorates the retina against light-induced damage in rats. Med Gas Res, 3(1):19.

[101]Vignais PM, Billoud B, Meyer J, 2001. Classification and phylogeny of hydrogenases. FEMS Microbiol Rev, 25(4):455-501.

[102]Vogt S, Lyon EJ, Shima S, et al., 2008. The exchange activities of [Fe] hydrogenase (iron–sulfur-cluster-free hydrogenase) from methanogenic archaea in comparison with the exchange activities of [FeFe] and [NiFe] hydrogenases. J Biol Inorg Chem, 13(1):97-106.

[103]Volgusheva A, Styring S, Mamedov F, 2013. Increased photosystem II stability promotes H₂ production in sulfur-deprived Chlamydomonas reinhardtii. Proc Natl Acad Sci USA, 110(18):7223-7228.

[104]Wang F, Yu G, Liu SY, et al., 2011. Hydrogen-rich saline protects against renal ischemia/reperfusion injury in rats. J Surg Res, 167(2):e339-e344.

[105]Wang YP, Wei C, 2016. Effect of hydrogen-rich water concentration on rooting of Nepenthes. Mod Agric Sci Technol, (14):136-137 (in Chinese).

[106]Wu Q, Su NN, Cai JT, et al., 2015. Hydrogen-rich water enhances cadmium tolerance in Chinese cabbage by reducing cadmium uptake and increasing antioxidant capacities. J Plant Physiol, 175:174-182.

[107]Xie YJ, Mao Y, Lai DW, et al., 2012. H₂ enhances Arabidopsis salt tolerance by manipulating ZAT10/12-mediated antioxidant defence and controlling sodium exclusion. PLoS ONE, 7(11):e49800.

[108]Xie YJ, Mao Y, Zhang W, et al., 2014. Reactive oxygen species-dependent nitric oxide production contributes to hydrogen-promoted stomatal closure in Arabidopsis. Plant Physiol, 165(2):759-773.

[109]Xie YJ, Zhang W, Duan XL, et al., 2015. Hydrogen-rich water-alleviated ultraviolet-B-triggered oxidative damage is partially associated with the manipulation of the metabolism of (iso)flavonoids and antioxidant defence in Medicago sativa. Funct Plant Biol, 42(12):1141-1157.

[110]Xu DK, Cao H, Fang W, et al., 2017. Linking hydrogen-enhanced rice aluminum tolerance with the reestablishment of GA/ABA balance and miRNA-modulated gene expression: a case study on germination. Ecotoxicol Environ Saf, 145:303-312.

[111]Xu LL, Fan JH, Wang QX, 2019. Omics application of bio-hydrogen production through green alga Chlamydomonas reinhardtii. Front Bioeng Biotechnol, 7:201.

[112]Xu S, Zhu SS, Jiang YL, et al., 2013. Hydrogen-rich water alleviates salt stress in rice during seed germination. Plant Soil, 370(1-2):47-57.

[113]Yagi T, Higuchi Y, 2013. Studies on hydrogenase. Proc Jpn Acad Ser B Phys Biol Sci, 89(1):16-33.

[114]Yang CH, Crowley DE, 2000. Rhizosphere microbial community structure in relation to root location and plant iron nutritional status. Appl Environ Microbiol, 66(1):345-351.

[115]Yang T, Jin ZK, Wang ZH, et al., 2018. Intratumoral high-payload delivery and acid-responsive release of H₂ for efficient cancer therapy using the ammonia borane-loaded mesoporous silica nanomedicine. Appl Mater Today, 11: 136-143.

[116]Young IM, Crawford JW, 2004. Interactions and self-organization in the soil-microbe complex. Science, 304(5677):1634-1637.

[117]Zeng JQ, Zhang MY, Sun XJ, 2013. Molecular hydrogen is involved in phytohormone signaling and stress responses in plants. PLoS ONE, 8(8):e71038.

[118]Zhang JJ, Hao HB, Chen MJ, et al., 2017. Hydrogen-rich water alleviates the toxicities of different stresses to mycelial growth in Hypsizygus marmoreus. AMB Express, 7(1):107.

[119]Zhang XY, Wei JY, Huang YF, et al., 2018. Increased cytosolic calcium contributes to hydrogen-rich water-promoted anthocyanin biosynthesis under UV-A irradiation in radish sprouts hypocotyls. Front Plant Sci, 9:1020.

[120]Zhang YH, Zhao G, Cheng PF, et al., 2019. Nitrite accumulation during storage of tomato fruit as prevented by hydrogen gas. Int J Food Prop, 22(1):1425-1438.

[121]Zhao L, Wang YB, Qin SR, et al., 2013. Protective effect of hydrogen-rich saline on ischemia/reperfusion injury in rat skin flap. J Zhejiang Univ-Sci B (Biomed & Biotechnol), 14(5):382-391.

[122]Zhao PH, Jin ZK, Chen Q, et al., 2018. Local generation of hydrogen for enhanced photothermal therapy. Nat Commun, 9(1):4241.

[123]Zhu YC, Liao WB, 2017. The metabolic constituent and rooting-related enzymes responses of marigold explants to hydrogen gas during adventitious root development. Theor Exp Plant Physiol, 29(2):77-85.

[124]Zhu YC, Liao WB, Wang M, et al., 2016. Nitric oxide is required for hydrogen gas-induced adventitious root formation in cucumber. J Plant Physiol, 195:50-58.

[125]List of electronic supplementary materials

[126]Table S1 Articles about hydrogen agronomy