Journal of Zhejiang University SCIENCE B 2026 Vol.27 No.8 P.923-926

http://doi.org/10.1631/jzus.B2500729


Development and application of a novel TaqMan qPCR assay targeting the gN gene for genotyping pseudorabies virus


Author(s):  Yulan JIN, Xuan JI, Weiren DONG, Yanming HUANG, Jiyong ZHOU, Jinyan GU

Affiliation(s):  1. The Experimental Teaching Center, College of Animal Sciences, Zhejiang University, Hangzhou 310058, China more

Corresponding email(s):   gujinyan@zju.edu.cn

Key Words:  Pseudorabies virus, Glycoprotein N, Taqman qPCR, Genotyping, Detection methods


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Yulan JIN, Xuan JI, Weiren DONG, Yanming HUANG, Jiyong ZHOU, Jinyan GU. Development and application of a novel TaqMan qPCR assay targeting the gN gene for genotyping pseudorabies virus[J]. Journal of Zhejiang University Science B, 2026, 27(8): 923-926.

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journal="Journal of Zhejiang University Science B",
volume="27",
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year="2026",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B2500729"
}

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Abstract: 
Pseudorabies virus (PRV), known as porcine herpesvirus type I, belongs to the α-herpesvirus subfamily of the herpesviridae family. Its virions are oval or round, with a genome of approximately 143 000 bp and a high guanine-cytosine (GC) content of 73% (Pomeranz et al., 2005). While swine are the natural host and the virus has caused significant economic loss to the pig industry, PRV can infect other mammals, causing acute infectious diseases characterized by neurological symptoms (He et al., 2019; Cheng et al., 2020). Young pigs are more susceptible to PRV and the infection in piglets is nearly 100% fatal, whereas adult pigs exhibit reproductive disorders such as abortion, stillbirths, and reduced fertility, ultimately decreasing farm productivity (Zuckermann, 2000). Due to its global impact, PRV is classified as a Class B infectious disease by the World Organization for Animal Health (WOAH).

基于gN基因的伪狂犬病病毒TaqMan qPCR分型方法的建立及应用

金玉兰1,2, 季暄1, 董伟仁1,2, 黄彦明1,2, 周继勇1,2, 顾金燕1,2
1浙江大学动物科学学院实验教学中心, 中国杭州, 310058
2 浙江大学动物医学中心农业农村部动物病毒学重点实验室, 中国杭州, 310058
摘要:伪狂犬病是由伪狂犬病病毒(PRV)引起的一种严重危害全球养猪业的传染病,由于不同基因型毒株的致病性与流行特征存在差异,临床上需建立一种能够快速、准确区分PRV基因I型和基因II型的分型检测方法。本研究首次以gN基因为靶标,基于其保守区域鉴别稳定差异序列,成功建立了一种TaqMan三重实时荧光定量聚合酶链式反应(PCR)检测方法。该方法不仅可检测PRV感染,而且能有效区分其基因型。该检测方法具有特异性强、灵敏度高、重复性好及操作简便等优点。该检测体系仅需一对引物和三个探针即可完成分型鉴定,极大简化了反应体系,降低了引物错配及非特异性扩增的风险。对实验动物来源样本初步应用的结果显示,该方法能准确检测PRV的感染情况及其基因型。本研究为PRV的分子流行病学监测提供了实用、高效的技术工具,也为猪群中PRV的净化工作提供了有力支持。

关键词:伪狂犬病病毒(PRV);gN基因;TaqMan;聚合酶链式反应(PCR);分型;检测方法

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Reference

[1]AnTQ, PengJM, TianZJ, et al., 2013. Pseudorabies virus variant in Bartha-K61‒vaccinated pigs, China, 2012. Emerg Infect Dis, 19(11):1749-1755.

[2]ChengZL, KongZJ, LiuP, et al., 2020. Natural infection of a variant pseudorabies virus leads to bovine death in China. Transbound Emerg Dis, 67(2):518-522.

[3]DelvaJL, NauwynckHJ, MettenleiterTC, et al., 2020. The attenuated pseudorabies virus vaccine strain Bartha K61: a brief review on the knowledge gathered during 60 years of research. Pathogens, 9(11):897.

[4]DingSX, ShenTR, FengZX, et al., 2024. Development of a highly sensitive TaqMan method based on multi-probe strategy: its application in ASFV detection. Biol Methods Protoc, 9(1):bpae011.

[5]HeWT, AuclertLZ, ZhaiXF, et al., 2019. Interspecies transmission, genetic diversity, and evolutionary dynamics of pseudorabies virus. J Infect Dis, 219(11):1705-1715.

[6]JinYL, YinD, XingG, et al., 2022. The inactivated gE/TK gene-deleted vaccine against pseudorabies virus type II confers effective protection in mice and pigs. Front Microbiol, 13:943707.

[7]MorenkovOS, SobkoYA, PanchenkoOA, 1997. Glycoprotein gE blocking ELISAs to differentiate between Aujeszky’s disease-vaccinated and infected animals. J Virol Methods, 65(1):83-94.

[8]PomeranzLE, ReynoldsAE, HengartnerCJ, 2005. Molecular biology of pseudorabies virus: impact on neurovirology and veterinary medicine. Microbiol Mol Biol Rev, 69(3):462-500.

[9]SongCL, GaoLB, BaiWB, et al., 2017. Molecular epidemiology of pseudorabies virus in Yunnan and the sequence analysis of its gD gene. Virus Genes, 53(3):392-399.

[10]State Administration for Market Regulation, Standardization Administration of China, 2018. Diagnostic Method for Pseudorabies, GB/T 18641-2018. Standards Press of China, Beijing, China.

[11]TanL, YaoJ, YangYD, et al., 2021. Current status and challenge of pseudorabies virus infection in China. Virol Sin, 36(4):588-607.

[12]TuF, ZhangYN, XuSK, et al., 2022. Detection of pseudorabies virus with a real-time recombinase-aided amplification assay. Transbound Emerg Dis, 69(4):2266-2274.

[13]WuXM, ChenRJ, ChenQY, et al., 2023. Establishment of an indirect ELISA method for antibody detection of porcine pseudorabies by recombinant gB, gC, and gD proteins. J Med Virol, 95(1):e28228.

[14]ZhaiXF, ZhaoW, LiKM, et al., 2019. Genome characteristics and evolution of pseudorabies virus strains in Eastern China from 2017 to 2019. Virol Sin, 34(6):601-609.

[15]ZhengHH, FuPF, ChenHY, et al., 2022. Pseudorabies virus: from pathogenesis to prevention strategies. Viruses, 14(8):1638.

[16]ZuckermannFA, 2000. Aujeszky’s disease virus: opportunities and challenges. Vet Res, 31(1):121-131.

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Full Text:   <603>

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On-line Access: 2026-08-13

Received: 2025-11-13

Revision Accepted: 2026-02-24

Crosschecked: 2026-08-13

Cited: 0

Clicked: 565

Citations:  Bibtex RefMan EndNote GB/T7714

 ORCID:

Yulan JIN

https://orcid.org/0000-0002-7752-5062

Jinyan GU

https://orcid.org/0000-0002-9763-9569

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