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On-line Access: 2024-08-27
Received: 2023-10-17
Revision Accepted: 2024-05-08
Crosschecked: 2023-11-25
Cited: 0
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A novel integrated microfluidic chip for on-demand electrostatic droplet charging and sorting
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Jinhui Yao, Chunhua He, Jianxin Wang, Canfeng Yang, Ye Jiang, Zhiyong Liu, Guanglan Liao & Tielin Shi . A novel integrated microfluidic chip for on-demand electrostatic droplet charging and sorting[J]. Journal of Zhejiang University Science D, 2024, 7(1): 31-42.
@article{title="A novel integrated microfluidic chip for on-demand electrostatic
droplet charging and sorting",
author="Jinhui Yao, Chunhua He, Jianxin Wang, Canfeng Yang, Ye Jiang, Zhiyong Liu, Guanglan Liao & Tielin Shi ",
journal="Journal of Zhejiang University Science D",
volume="7",
number="1",
pages="31-42",
year="2024",
publisher="Zhejiang University Press & Springer",
doi="10.1007/s42242-023-00257-z"
}
%0 Journal Article
%T A novel integrated microfluidic chip for on-demand electrostatic
droplet charging and sorting
%A Jinhui Yao
%A Chunhua He
%A Jianxin Wang
%A Canfeng Yang
%A Ye Jiang
%A Zhiyong Liu
%A Guanglan Liao & Tielin Shi
%J Journal of Zhejiang University SCIENCE D
%V 7
%N 1
%P 31-42
%@ 1869-1951
%D 2024
%I Zhejiang University Press & Springer
%DOI 10.1007/s42242-023-00257-z
TY - JOUR
T1 - A novel integrated microfluidic chip for on-demand electrostatic
droplet charging and sorting
A1 - Jinhui Yao
A1 - Chunhua He
A1 - Jianxin Wang
A1 - Canfeng Yang
A1 - Ye Jiang
A1 - Zhiyong Liu
A1 - Guanglan Liao & Tielin Shi
J0 - Journal of Zhejiang University Science D
VL - 7
IS - 1
SP - 31
EP - 42
%@ 1869-1951
Y1 - 2024
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1007/s42242-023-00257-z
Abstract: On-demand droplet sorting is extensively applied for the efficient manipulation and genome-wide analysis of individual
cells. However, state-of-the-art microfluidic chips for droplet sorting still suffer from low sorting speeds, sample loss, and
labor-intensive preparation procedures. Here, we demonstrate the development of a novel microfluidic chip that integrates
droplet generation, on-demand electrostatic droplet charging, and high-throughput sorting. The charging electrode is a copper
wire buried above the nozzle of the microchannel, and the deflecting electrode is the phosphate buffered saline in the
microchannel, which greatly simplifies the structure and fabrication process of the chip. Moreover, this chip is capable
of high-frequency droplet generation and sorting, with a frequency of 11.757 kHz in the drop state. The chip completes
the selective charging process via electrostatic induction during droplet generation. On-demand charged microdroplets can
arbitrarily move to specific exit channels in a three-dimensional (3D)-deflected electric field, which can be controlled according
to user requirements, and the flux of droplet deflection is thereby significantly enhanced. Furthermore, a lossless modification
strategy is presented to improve the accuracy of droplet deflection or harvest rate from 97.49% to 99.38% by monitoring
the frequency of droplet generation in real time and feeding it back to the charging signal. This chip has great potential for
quantitative processing and analysis of single cells for elucidating cell-to-cell variations.
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