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On-line Access: 2024-08-27
Received: 2023-10-17
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Electrospun polyvinyl alcohol fibres incorporating an antimicrobial gel for enzymatically controlled reactive oxygen species release
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Joel Yupanqui Mieles , Cian Vyas, Evangelos Daskalakis , Mohamed Hassan , James Birkett , Abdalla M. Omar , Gavin Humphreys , Carl Diver , Paulo Bartolo. Electrospun polyvinyl alcohol fibres incorporating an antimicrobial gel for enzymatically controlled reactive oxygen species release[J]. Journal of Zhejiang University Science D, 2024, 7(6): 899-925.
@article{title="Electrospun polyvinyl alcohol fibres incorporating an antimicrobial gel
for enzymatically controlled reactive oxygen species release",
author="Joel Yupanqui Mieles , Cian Vyas, Evangelos Daskalakis , Mohamed Hassan , James Birkett , Abdalla M. Omar , Gavin Humphreys , Carl Diver , Paulo Bartolo",
journal="Journal of Zhejiang University Science D",
volume="7",
number="6",
pages="899-925",
year="2024",
publisher="Zhejiang University Press & Springer",
doi="10.1007/s42242-024-00312-3"
}
%0 Journal Article
%T Electrospun polyvinyl alcohol fibres incorporating an antimicrobial gel
for enzymatically controlled reactive oxygen species release
%A Joel Yupanqui Mieles
%A Cian Vyas
%A Evangelos Daskalakis
%A Mohamed Hassan
%A James Birkett
%A Abdalla M. Omar
%A Gavin Humphreys
%A Carl Diver
%A Paulo Bartolo
%J Journal of Zhejiang University SCIENCE D
%V 7
%N 6
%P 899-925
%@ 1869-1951
%D 2024
%I Zhejiang University Press & Springer
%DOI 10.1007/s42242-024-00312-3
TY - JOUR
T1 - Electrospun polyvinyl alcohol fibres incorporating an antimicrobial gel
for enzymatically controlled reactive oxygen species release
A1 - Joel Yupanqui Mieles
A1 - Cian Vyas
A1 - Evangelos Daskalakis
A1 - Mohamed Hassan
A1 - James Birkett
A1 - Abdalla M. Omar
A1 - Gavin Humphreys
A1 - Carl Diver
A1 - Paulo Bartolo
J0 - Journal of Zhejiang University Science D
VL - 7
IS - 6
SP - 899
EP - 925
%@ 1869-1951
Y1 - 2024
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1007/s42242-024-00312-3
Abstract: Wounds pose a risk to the skin, our bodys primary defence against infections. The rise of antibiotic resistance has prompted
the development of novel therapies. RO-101 is an antimicrobial gel that delivers therapeutic levels of hydrogen peroxide
(H2O2), a reactive oxygen species, directly to the wound bed. In this study, electrospinning was used to incorporate RO
101 into a polyvinyl alcohol (PVA) sub-micron fibrous mesh that can act as a delivery agent, achieve a sustained release
profile, and provide a barrier against infection. Adequate incorporation of this gel into sub-micron fibres was confirmed via
nuclear magnetic resonance spectroscopy. Furthermore, scanning electron microscopy exhibited smooth and uniform meshes
with diameters in the 200500 nm range. PVA/RO-101 electrospun meshes generated H2O2 in concentrations exceeding
1 mM/(gmL) (1 mM = 1 mmol/L) after 24 h, and the role of sterilisation on H2O2 release was evaluated. PVA/RO-101
meshes exhibited antimicrobial activity against both Gram-positive Staphylococcus aureus (S. aureus) and Gram-negative
Pseudomonas aeruginosa (P. aeruginosa) bacteria, achieving viable count reductions of up to 1 log unit CFU/mm2 (CFU:
colony-forming units). Moreover, these mesheswerecapableofdisruptingbiofilmformation,evenagainstmultidrug-resistant
organisms such as methicillin-resistant S. aureus (MRSA). Furthermore, increasing the RO-101 concentration resulted in
higher H2O2 production and an enhanced antimicrobial effect, while fibroblast cell viability and proliferation tests showed a
concentration-dependent response with high cytocompatibility at low RO-101 concentrations. This study therefore demon
strates the potential of highly absorbent PVA/RO-101 meshes as potential antimicrobial wound dressings.
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