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Abstract: The influence of KH₂PO₄, peptone and brown sugar on Termitomyces fuliginosu's mycelium formation was studied with the application of the design of quadratic rotation general combination based on determining the growth curve. A quadratic regression model of biomass to the doses of the above three factors was established. The model fit well and therefore the optimum fermentation condition was obtained. Responses of biomass to the three single factors and to their interactions were discussed. Thus, the highest level of biomass, 21.1 g/L, appeared under the optimized conditions when the initial KH₂PO₄, peptone and brown sugar were 1 g/L, 4 g/L and 72 g/L respectively.

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