Full Text:
<2719>
CLC number: Q814.3
On-line Access: 2024-08-27
Received: 2023-10-17
Revision Accepted: 2024-05-08
Crosschecked: 0000-00-00
Cited: 17
Clicked: 4824
Fiber optic biosensor of immobilized firefly luciferase
| |||||||||||||
CAI Jin, MENG Wen-fang, JI Xin-song. Fiber optic biosensor of immobilized firefly luciferase[J]. Journal of Zhejiang University Science A, 2002, 3(5): 563-566.
@article{title="Fiber optic biosensor of immobilized firefly luciferase",
author="CAI Jin, MENG Wen-fang, JI Xin-song",
journal="Journal of Zhejiang University Science A",
volume="3",
number="5",
pages="563-566",
year="2002",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.2002.0563"
}
%0 Journal Article
%T Fiber optic biosensor of immobilized firefly luciferase
%A CAI Jin
%A MENG Wen-fang
%A JI Xin-song
%J Journal of Zhejiang University SCIENCE A
%V 3
%N 5
%P 563-566
%@ 1869-1951
%D 2002
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.2002.0563
TY - JOUR
T1 - Fiber optic biosensor of immobilized firefly luciferase
A1 - CAI Jin
A1 - MENG Wen-fang
A1 - JI Xin-song
J0 - Journal of Zhejiang University Science A
VL - 3
IS - 5
SP - 563
EP - 566
%@ 1869-1951
Y1 - 2002
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.2002.0563
Abstract: Luciferase from firefly lantern extract was immobilized on CNBr-activated Sepharose 4B. The kinetic properties of immobilized luciferase were extensively studied. The Km′ for D-luciferin is 11.9 μmol/L, the optimum pH and temperature for Sepharose-bound enzyme were 7.8 and 25°C respectively. A luminescence fiber optic biosensor, making use of immobilized crude luciferase, was developed for assay of ATP. The peak light intensity was linear with respect to ATP concentration in range of 10-9–10-5 mol/L. A biological application was also demonstrated with the determination of serum ATP from rats bred in low versus normal oxygen environments.
[1] Blum, L.J., Gautier, S.M., Coulet, P.R., 1988. Luminescence fiber optic biosensor. Anal. Lett., 21: 717-726.
[2] Lagido, C., Pettitt, J., Porter, A.J.R., Paton, G.I., Glover, L.A., 2001. Development and application of bioluminescent Caenorhabditis elegans as multicellular eukaryotic biosensors. FEBS Letters, 493:36-39.
[3] Leach, F.R., 1981. ATP determination with firefly luciferase. J. Appl. Biochem., 3: 473-478.
[4] Lee,Y., Jablonski, I., Deluca, M., 1981. Immobilization of firefly luciferase on glass rods: Properties of the immobilized enzyme. Anal. Biochem., 80:496-500.
[5] Ji, X.S., Li, H.X., Yuan, Z.Y., Liu S.H., 1984. Immobilization of NAD kinase. Enzyme Engineering 7, Anal. N. Y. Acad. Sci., 434:264-266.
[6] Maria, P. X., Begona, V., Maria, D. M., 2000. Fiber optic monitoring of carbamate pesticides using porous glass with covalently bound chlorophenol red. Biosensors & Bioelectronics, 14:895-905.
[7] Michel, P.E., Gautier-Sauvigne, S.M., Blum, L.J., 1998. A transient enzymatic inhibition as an efficient tool for the discriminating bioluminescent analysis of three adenylic nucleotides with a fiberoptic sensor based on a compartmentalized tri-enzymatic sensing layer. Analytica Chimica Acta, 360: 89-99.
[8] Neuberg, C., 1944. Convenient method for deproteinization. Arch. Biochem. 4:101-105.
[9] Tservistas, M., Koneke, R., Comte, A. Scheper T., 2001. Oxygen monitoring in supercritical carbon dioxide using a fibre optic sensor. Enzyme and Microbial Technology, 28:637-634.
[10] Vangelis, G. A., Yannis, D. C., 2002. A portable fiber-optic pesticide biosensor based on immobilized cholinesterase and sol-gel entrapped bromcresol purple for in-field use. Biosensor & Bioelectronics, 17:61-69.
Open peer comments: Debate/Discuss/Question/Opinion
<1>