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Abstract: The production of elastase by bacillus sp. EL31410 at various temperatures was investigated. In order to study the effect of temperature on elastase fermentation, different cultivation temperatures, ranging from 39 °C to 28 °C, were evaluated in shake flask. The result indicated that 37 °C was best for cell growth at earlier stage; while maximum elastase activity was obtained when the cells were cultivated at 30 °C. This result was verified by batch fermentation in 5-L bioreactor under 37 °C and 30 °C temperature, respectively. The specific cell growth rate at 37 °C was higher than that at 30 °C during earlier stage of cultivation. The maximum value [5.5 U/(h·g DCW)] of elastase formation rate occurred at 24 h at 30 °C compared to 4.6 U/(h·g DCW) at 30 h at 37 °C. Based on these results, two-stage temperature shift strategy and oscillatory temperature cultivation mode were evaluated in the next study. When compared to single temperature of 37 °C or 30 °C, both two-stage temperature shift strategy and oscillatory temperature strategy improved biomass but did not yield the same result as expected for elastase production. The maximum biomass (both 8.6 g/L) was achieved at 30 h at 37 °C, but at 42 h using two-stage temperature cultivation strategy. The highest elastase production (652 U/ml) was observed at 30 °C in batch process. It was concluded that cultivation at constant temperature of 30 °C was appropriate for elastase production by bacillus sp. EL31410.

[1] Beg, Q.K., Saxena, R.K., Gupta, R., 2002. De-repression and subsequent induction of protease synthesis by Bacillus mojavensis under fed-batch operations. Process Biochemistry, 37:1103-1109.

[2] Chen, Q.H., He, G.Q., Molhtar, A.M.A., 2002. Optimization of medium composition for the production of elastase by Bacillus sp. EL31410 with response surface methodology. Enzyme Microbial Technol, 30:667-672.

[3] Chen, Q.H., He, G.Q., Schwarz, P., 2004. Studies on cultivation kinetics for elastase production by Bacillus sp. EL31410. J Agric Food Chem, 52:3356-3359.

[4] Chu, J., Li, Y.R., 2002. Regulation Technology of Modern Fermentation Industry. Chemistry Industry Press, Beijing, China, p.262-263 (in Chinese).

[5] Feng, Y.Y., He, Z.M., Say, L.O., Hu, J.Y., Zhang, Z.G., Win, J.N., 2003. Optimization of agitation, aeration, and temperature conditions for maximum (-mannanase production. Enzyme Microbial Technol, 32:282-289.

[6] Ke, N., Xiao, C.S., 2002. Research process of elastase produced by microorganism. Microbiology, 4:91-94 (in Chinese).

[7] Mei, L.H., Yao, S.J., Lin, D.Q., 1999. Biochemistry Process Technology. Science Press, Beijing, China, p.185-186 (in Chinese).

[8] Morihara, K., 1967. Elastolytic properties of various proteases from microbial origin. Arch Biochem Biophys, 120:68-78.

[9] Sachar, L.A., 1955. Photometry method for estimation of elastase activity. Proc Soc Expeti Biol Med, 90:323-325.

[10] Shibata, Y., Fujimura, S., Nakamura, T., 1993. Purification and partial characterization of an elastolytic serine protease of Prevotella intermedia. Appl Environm Microbial, 59:2107-2111.

[11] Tsai, Y.C., Jung, R.Y., Lin, S.F., 1988. Production and further characterization of an alkaline elastase production by alkalophilic Bacillus strain YaB. Appl Environm Microbial, 1:3156-3161.

[12] Zhang, L.X., Zhang, Y.F., Li, L.Y., 1997. Biochemistry Experiment and Technology. Advanced Education Press, Beijing, China, p.1-3 (in Chinese).

[13] Zhang, J.A., Wei, X.S., Xie, D.M., Sun, Y., Liu, D.H., 2002. Effects of oscillatory temperature and sparging nitrogen gas or carbon dioxide during later stage on glycerol fermentation. J Chemical Industry and Engineering, 53:980-983.

[14] Zheng, M.Y., Du, G.C., Guo, W.G., Chen, J., 2001. A temperature-shift strategy in batch microbial transglutaminase fermentation. Process Biochemistry, 36:525-530.

[15] Zins, M.M., Zimprich, A.C., Petermann, R.S., Rust, L., 2001. Expression and partial characterization of an elastase from Chromobacterium Violaceum. Veterinary Microbiology, 80:63-74.