CLC number: Q556+.5; Q786
On-line Access: 2024-08-27
Received: 2023-10-17
Revision Accepted: 2024-05-08
Crosschecked: 0000-00-00
Cited: 2
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Gong Xing-guo, Ji Jing, Xie Jie, Zhou Yuan, Zhang Jun-yan, Zhong Wen-tao. Expression, purification, and bioactivity of GST-fused v-Src from a bacterial expression system[J]. Journal of Zhejiang University Science B, 2006, 7(1): 13-19.
@article{title="Expression, purification, and bioactivity of GST-fused v-Src from a bacterial expression system",
author="Gong Xing-guo, Ji Jing, Xie Jie, Zhou Yuan, Zhang Jun-yan, Zhong Wen-tao",
journal="Journal of Zhejiang University Science B",
volume="7",
number="1",
pages="13-19",
year="2006",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.2006.B0013"
}
%0 Journal Article
%T Expression, purification, and bioactivity of GST-fused v-Src from a bacterial expression system
%A Gong Xing-guo
%A Ji Jing
%A Xie Jie
%A Zhou Yuan
%A Zhang Jun-yan
%A Zhong Wen-tao
%J Journal of Zhejiang University SCIENCE B
%V 7
%N 1
%P 13-19
%@ 1673-1581
%D 2006
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.2006.B0013
TY - JOUR
T1 - Expression, purification, and bioactivity of GST-fused v-Src from a bacterial expression system
A1 - Gong Xing-guo
A1 - Ji Jing
A1 - Xie Jie
A1 - Zhou Yuan
A1 - Zhang Jun-yan
A1 - Zhong Wen-tao
J0 - Journal of Zhejiang University Science B
VL - 7
IS - 1
SP - 13
EP - 19
%@ 1673-1581
Y1 - 2006
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.2006.B0013
Abstract: v-Src is a non-receptor protein tyrosine kinase involved in many signal transduction pathways and closely related to the activation and development of cancers. We present here the expression, purification, and bioactivity of a GST (glutathione S-transferase)-fused v-Src from a bacterial expression system. Different culture conditions were examined in an isopropyl β-D-thiogalactopyranoside (IPTG)-regulated expression, and the fused protein was purified using GSH (glutathione) affinity chromatography. ELISA (enzyme-linked immunosorbent assay) was employed to determine the phosphorylation kinase activity of the GST-fused v-Src. This strategy seems to be more promising than the insect cell system or other eukaryotic systems employed in earlier Src expression.
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