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Abstract: Screening of microorganisms producing flocculating substances was carried out. A strain secreting a large amount of bioflocculant was isolated from wastewater samples collected from the Little Moon River in Beijing. Based on the morphological properties and 16S rDNA sequence analysis, the isolate (designated W31) was classified as Vagococcus sp. A bioflocculant (named MBFW31) produced by W31 was extracted from the culture broth by ethanol precipitation and purified by gel chromatography. MBFW31 was heat-stable and had strong flocculating activity in a wide range of pH with relatively low dosage requirement. MBFW31 was identified as a polysaccharide with molecular weight over 2×10⁶. It contained neutral sugar and uronic acid as its major and minor components, respectively. Infrared spectra showed the presence of hydroxyl, carboxyl and methoxyl group in its molecules. The present results suggested that MBFW31 had potential application in wastewater treatment.

[1] Bradford, M.M., 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem., 72(1-2):248-254.

[2] Chang, W.C., Soon, A.Y., In, H.O., Sang, H.P., 1998. Characterization of an extracellular flocculating substance produced by a planktonic cyanobacterium, Anabaena sp. Biotechnol. Lett., 20(12):643-646.

[3] Chaplin, M.F., 1986. Monosaccharides. In: Chaplin, M.F., Kennedy, J.F. (Eds.), Carbohydrate Analysis: a Practical Approach. IRL Press, Oxford, UK, p.2-5.

[4] Chen, M., Zhao, L.P., 2003. Biodiversity of bacterial isolates on three different media from coking wastewater treatment system. Acta Microbiol. Sin., 43:367-371 (in Chinese).

[5] Chen, H., Zhang, J.F., Jiang, P.J., Yang, S.L., Liu, Z.L., 2002. Composition and characterization of microbiological flocculant SC06. Environ. Chem., 21:360-364 (in Chinese).

[6] Deng, S.B., Bai, R.B., Hu, X.M., Luo, Q., 2003. Characteristics of a bioflocculant produced by Bacillus mucilaginosus and its use in starch wastewater treatment. Appl. Microbiol. Biotechnol., 60:588-593.

[7] Dermlim, W., Prasertsan, P., Doelle, H., 1999. Screening and characterization of bioflocculant produced by isolated Klebsiella sp. Appl. Microbiol. Biotechnol., 52(5):698-703.

[8] Gupta, R., Lanter, J.M., Woese, C.R., 1983. Sequence of the 16S ribosomal RNA from Halobacterium volcanii, an archaebacterium. Science, 221:656-659.

[9] He, N., Li, Y., Chen, J., Shi, Y.L., 2002. Identification of a novel bioflocculant from a newly isolated Corynrbaaterium glutamicum. Biochem. Eng. J., 11(2-3):137-148.

[10] He, N., Li, Y., Chen, J., 2004. Production of a novel polygalacturonic acid biofloccualnt REA-11 by Corynebacterium glutamicum. Bioresour. Technol., 94(1):99-105.

[11] James, K.C., 1986. Solubility and the Related Properties. In: Drugs and the Pharmaceutical Sciences, Vol. 28. Marcel Dekker, New York, USA, p.91-125.

[12] Koizumi, J.I., Tskeda, M., Kurane, R., Nakamura, J., 1991. Synergetic flocculation of the bioflocculant FIX extracellularly produced by Nocardia amarae. J. Gen. Appl. Microbiol., 37:447-454.

[13] Kumar, C.G., Joo, H.S., Kavali, R., Choi, J.W., Chang, C.S., 2004. Characterization of an extracellular biopolymer flocculant from a haloakalophilic Bacillus isolate. World J. Micro. Biotech., 20(8):837-843.

[14] Kurane, R., Takeda, K., Suzuki, T., 1986. Screening and characteristics of microbial flocculants. Agric. Biol. Chem., 50:2301-2307.

[15] Kwon, G.S., Moon, S.H., Hong, S.D., Lee, H.M., Kim, H.S., Oh, H.M., Yoon, B.D., 1996. A novel flocculant biopolymer produced by Pestalotiopsis sp. KCTC 8637p. Biotechnol. Lett., 18(12):1459-1464.

[16] Lee, S.H., Lee, S.O., Jang, K.L., Lee, T.H., 1995. Microbial flocculant from Arcuadendron sp. TS-49. Biotechnol. Lett., 17(1):95-100.

[17] Michaels, A.S., 1954. Aggregation of suspensions by polyelectrolytes. Ind. Eng. Chem., 46(7):1485.

[18] Rainey, F.A., Ward-Rainey, N., Kroppenstedt, R.M., Stackebrandt, E., 1996. The genus Nocardiopsis represents a phylogenetically cohere taxon and a distince actinomycete lineage: proposal of Nocardiopsaceae fam. nov. Int. J. Syst. Bacteriol., 46:1088-1092.

[19] Salehizadeh, H., Shojaosadati, S.A., 2001. Extracellular biopolymeric flocculants: recent trends and biotechnology importance. Biotechnol. Adv., 19(5):371-385.

[20] Sheng, G.P., Han, Q.Y., Yu, Z., 2005. Extraction of extracellular polymeric substances from the photosynthesis bacterium Rhodopseudomonas acidophila. Appl. Microbiol. Biotechnol., 67(1):125-130.

[21] Shih, I.L., Van, Y.T., Yeh, L.C., Lin, H.G., Chang, Y.N., 2001. Production of a biopolymer flocculant from Bacillus licheniformis and its flocculation properties. Bioresour. Technol., 78(3):267-272.

[22] Suh, H.H., Kwon, G.S., Lee, C.H., Kim, H.S., Oh, H.M., Yoon, B.D., 1997. Characterization of bioflocculant produced by Bacillus sp. DP-152. J. Ferment. Bioeng., 84(2):108-112.

[23] Tago, Y., Aida, K.O., 1977. Extracellular mucopolysaccharide closely related to bacterial floc formation. Appl. Environ. Microbiol., 34:308-314.

[24] Takeda, M., Kurane, R., 1991. A protein bioflocculant produced by Rhodococcus erythropolis. Agric. Biol. Chem., 55:2663-2664.

[25] Tenney, M.W., Verhoff, F.H., 1973. Chemical and autoflocculation of microorganisms in biological wastewater treatment. Biotechnol. Bioeng., 15(6):1045-1073.

[26] Toeda, K., Kurane, R., 1991. Microbial flocculant from Alcaligenes cupidus KT201. Agric. Biol. Chem., 55:2793-2799.

[27] Wang, Z., Wang, K.X., Xie, Y.M., Yao, Y.L., 1995. Bioflocculant-producing microorganisms. Acta Microbiol. Sin., 35:121-129 (in Chinese).

[28] Yokoi, H., Arima, T., Hayashi, S., Takasaki, Y., 1996. Flocculation properties of poly(gamma-glutamic acid) produced by Bacillus subtilis. J. Ferment. Bioeng., 82(1):84-87.

[29] Yoon, J.H., Cho, Y.G., Kang, S.S., Kim, S.B., Lee, S.T., Park, Y.H., 2000. Rhodococcus koreensis sp. nov., a 2, 4-dinitrophenol-degrading bacterium. Int. J. Syst. Bacteriol., 50:1193-1201.