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Abstract: An experiment was conducted on Catharanthus roseus to study the effect of seed treatments with native diazotrophs on its seedling growth and antioxidant enzyme activities. The treatments had significant influence on various seedling parameters. There is no significant influence on dry matter production with the diazotrophs, Azospirillum and Azotobacter. However, the vital seedling parameters such as germination percentage and vigour index were improved. Azotobacter treatment influenced maximum of 50% germination, whereas Azospirillum and Azotobacter were on par with C. roseus with respect to their vigour index. There was significant difference in the population of total diazotrophs. Azospirillum and Azotobacter between rhizosphere and non-rhizosphere soils of C. roseus had the same trend and were observed at various locations of the study. The activities of antioxidant enzymes such as superoxide dismutase (SOD), peroxidase (POX) and catalase (CAT) were increased to a significant extent due to the treatment with diazotrophs.

[1] Abdul-Baki, A.A., Anderson, J.D., 1973. Vigour determination in soybean seed by multiple criteria. Crop Sci., 13:630-633.

[2] Beauchamp, C.O., Fridovich, I., 1971. Superoxide dismutase: improved assays and an assay applicable to acrylamide gels. Anal. Biochem., 44(1):276-287.

[3] Bradford, M.M., 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding. Anal. Biochem., 72(1-2):248-253.

[4] Brown, M.E., Burlingham, S.K., 1968. Production of plant growth substances by Azotobacter chroococcum. J. Gen. Microbiol., 53:135-144.

[5] Chandlee, J.M., Scandalios, J.G., 1984. Analysis of variants affecting the catalase development program in maize scutellum. Theor. Appl. Genet., 69(1):71-77.

[6] Chaparzadeh, N., Amico, M.L., Nejad, R.K., Izzo, R., Izzo, F.N., 2004. Antioxidative responses of Calendula officinalis under salinity conditions. Plant Physiol. Biochem., 42(9):695-701.

[7] Deka, B.C., Bora, G.C., Shadeque, A., 1992. Effect of Azospirillum on growth and yield of chilli (Capsicum annuum L.) cultivar Pusa Jawala. Haryana J. Hort. Sci., 38:41-46.

[8] Govindarajan, K., Kavitha, K., 2001. Studies on Azospirillum Associated with Rice Varities. Workshop on Recent Developments in Biofertilizers for Rice-Based Cropping System, Coimbatore, p.9-10.

[9] Hernandez, J.A., Almansa, M.S., 2002. Short-term effects of salt stress on antioxidant systems and leaf water relations of pea plants. Physiol. Plant., 115(2):251-257.

[10] Imlay, J.A., 2003. Pathways of oxidative damage. Annu. Rev. Microbiol., 57(1):395-418.

[11] ISTA (International Seed Testing Association), 1976. International rules for seed testing. Seed Sci. Tech., 4:52-70.

[12] Jaleel, C.A., Gopi, R., Lakshmanan, G.M.A., Panneerselvam, R., 2006. Triadimefon induced changes in the antioxidant metabolism and ajmalicine production in Catharanthus roseus (L.) G. Don. Plant Sci., 171(2):271-276.

[13] Jaleel, C.A., Gopi, R., Sankar, B., Manivannan, P., Kishorekumar, A., Sridharan, R., Panneerselvam, R., 2007. Studies on germination, seedling vigour, lipid peroxidation and proline metabolism in Catharanthus roseus seedlings under salt stress. South African Journal of Botany, 73(2):190-195.

[14] Kar, A., Choudhary, B.K., Bandyopadhyay, N.G., 2003. Comparative evaluation of hypoglycemic activity of some Indian medicinal plants in alloxan diabetic rats. J. Ethnopharmacol., 84(1):105-108.

[15] Kumar, K.B., Khan, P.A., 1982. Peroxidase and polyphenol oxidase in excised ragi (Eleusine coracana cv. PR 202) leaves during senescence. Ind. J. Exp. Bot., 20:412-416.

[16] Lakshmanan, A., Govindarajan, K., Kumar, K., 2005. Effect of seed treatment with native diazotrophs on the seedling parameters of Senna and Ashwagandha. Crop Res., 30(1):119-123.

[17] Lin, C.C., Kao, C.H., 2000. Effect of NaCl stress on H₂O₂ metabolism in rice leaves. Plant Growth Regul., 30(2):151-155.

[18] Magnotta, M., Murata, J., Chen, J., de Luca, V., 2006. Identification of a low vindoline accumulating cultivar of Catharanthus roseus (L.) G. Don. by alkaloid and enzymatic profiling. Phytochemistry, 67(16):1758-1764.

[19] Muthukumarasamy, M., Dutta Gupta, S., Panneerselvam, R., 2000. Enhancement of peroxidase, polyphenol oxidase and superoxide dismutase activities by triadimefon in NaCl stressed Raphanus sativus L. Biol. Plant., 43(2):317-320.

[20] Nurnberger, T., Colling, C., Hahlbrock, K., Jabs, T., Renelt, A., Sacks, W.R., Scheel, D., 1994. Perception and transduction of an elicitor signal in cultured parsley cells. Biochem. Soc. Symp., 60:173-182.

[21] Prochazkova, D., Sairam, R.K., Srivastava, G.C., Singh, D.V., 2001. Oxidative stress and antioxidant activity as the basis of senescence in maize leaves. Plant Sci., 161(4):765-771.

[22] Reddy, A.R., Chiatanya, K.V., Vivekanandan, M., 2004. Draught induced responses of photosynthesis and antioxidant metabolism in higher plants. J. Plant Physiol., 161(11):1189-1202.

[23] Sairam, R.K., Veerabhadra Rao, K., Srivastava, G.C., 2002. Differential response of wheat genotypes to long term salinity stress in relation to oxidative stress, antioxidant activity and osmolyte concentration. Plant Sci., 163(5):1037-1046.

[24] Somssich, I.E., Hahlbrock, K., 1998. Pathogen defence in plant—a paradigm of biological complexity. Trends Plant Sci., 3(3):86-90.

[25] Verpoorte, R., Contin, A., Memelink, J., 2002. Biotechnology for the production of plant secondary metabolites. Phytochem. Rev., 1(1):13-25.

[26] Vranova, E., Inze, D., van Brensegem, F., 2002. Signal transduction during oxidative stress. J. Exp. Bot., 53(372):1227-1236.

[27] Watanabe, I., Barraquio, W.L., 1979. Low levels of fixed nitrogen are required for isolation of free-living nitrogen fixing organisms from rice roots. Nature, 277(5697):565-566.

[28] Zhao, J., Lawrence, T., Davis, C., Verpoorte, R., 2005. Elicitor signal transduction leading to production of plant secondary metabolites. Biotechnol. Adv., 23(4):283-333.