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Abstract: Objective: To explore the effects of insulin-like growth factor-1 (IGF-1) on migration, proliferation and differentiation of mesenchymal stem cells (MSCs). Methods: MSCs were obtained from Sprague-Dawley rats by a combination of gradient centrifugation and cell culture techniques and treated with IGF-1 at concentrations of 5–20 ng/ml. proliferation of MSCs was determined as the mean doubling time. Expression of CXC chemokine receptor 4 (CXCR4) and migration property were determined by flow cytometry and transwell migration essay, respectively. mRNA expression of GATA-4 and collagen II was determined by reverse transcription-polymerase chain reaction (RT-PCR). Results: The mean doubling time of MSC proliferation was decreased, and the expression of CXCR4 on MSCs and migration of MSCs were increased by IGF-1, all in a dose-dependent manner, while the optimal concentration of IGF-1 on proliferation and migration was different. IGF-1 did not affect the expression of GATA-4 or collagen II mRNA. Conclusions: IGF-1 dose-dependently stimulated the proliferation of MSCs, upregulated the expression of CXCR4, and accelerated migration. There was no apparent differentiation of MSCs to cardiomyocytes or chondrocytes after culturing with IGF-1 alone.

[1]Bartunek, J., Croissant, J.D., Wijns, W., Gofflot, S., de Lavareille, A., Vanderheyden, M., Kaluzhny, Y., Mazouz, N., Willemsen, P., Penicka, M., et al., 2007. Pretreatment of adult bone marrow mesenchymal stem cells with cardiomyogenic growth factors and repair of the chronically infarcted myocardium. Am. J. Physiol. Heart Circ. Physiol., 292(2):H1095-H1104.

[2]Chen, S.L., Fang, W.W., Ye, F., Liu, Y.H., Qian, J., Shan, S.J., Zhang, J.J., Chunhua, R.Z., Liao, L.M., Lin, S., et al., 2004. Effect on left ventricular function of intracoronary transplantation of autologous bone marrow mesenchymal stem cell in patients with acute myocardial infarction. Am. J. Cardiol., 94(1):92-95.

[3]Guo, J., Lin, G., Bao, C., Hu, Z., Chu, H., Hu, M., 2008. Insulin-like growth factor 1 improves the efficacy of mesenchymal stem cells transplantation in a rat model of myocardial infarction. J. Biomed. Sci., 15(1):89-97.

[4]Kaplan, R.C., Strickler, H.D., Rohan, T.E., Muzumdar, R., Brown, D.L., 2005. Insulin-like growth factors and coronary heart disease. Cardiol. Rev., 13(1):35-39.

[5]Katritsis, D.G., Sotiropoulou, P.A., Karvouni, E., Karabinos, I., Korovesis, S., Perez, S.A., Voridis, E.M., Papamichail, M., 2005. Transcoronary transplantation of autologous mesenchymal stem cells and endothelial progenitors into infarcted human myocardium. Catheter. Cardiovasc. Interv., 65(3):321-329.

[6]Kawamura, K., Chu, C.R., Sobajima, S., Robbins, P.D., Fu, F.H., Izzo, N.J., Niyibizi, C., 2005. Adenoviral-mediated transfer of TGF-β1 but not IGF-1 induces chondrogenic differentiation of human mesenchymal stem cells in pellet cultures. Exp. Hematol., 33(8):865-872.

[7]Li, Y., Yu, X., Lin, S., Li, X., Zhang, S., Song, Y.H., 2007. Insulin-like growth factor 1 enhances the migratory capacity of mesenchymal stem cells. Biochem. Biophys. Res. Commun., 356(3):780-784.

[8]Longobardi, L., O′Rear, L., Aakula, S., Johnstone, B., Shimer, K., Chytil, A., Horton, W.A., Moses, H.L., Spagnoli, A., 2006. Effect of IGF-I in the chondrogenesis of bone marrow mesenchymal stem cells in the presence or absence of TGF-β signaling. J. Bone Miner. Res., 21(4):626-636.

[9]Majumdar, M.K., Keane-Moore, M., Buyaner, D., Hardy, W.B., Moorman, M.A., McIntosh, K.R., Mosca, J.D., 2003. Characterization and functionality of cell surface molecules on human mesenchymal stem cells. J. Biomed. Sci., 10(2):228-241.

[10]Muguruma, Y., Reyes, M., Nakamura, Y., Sato, T., Matsuzawa, H., Miyatake, H., Akatsuka, A., Itoh, J., Yahata, T., Ando, K., et al., 2003. In vivo and in vitro differentiation of myocytes from human bone marrow-derived multipotent progenitor cells. Exp. Hematol., 31(12):1323-1330.

[11]Nagaya, N., Kangawa, K., Itoh, T., Iwase, T., Murakami, S., Miyahara, Y., Fujii, T., Uematsu, M., Ohgushi, H., Yamagishi, M., et al., 2005. Transplantation of mesenchymal stem cells improves cardiac function in a rat model of dilated cardiomyopathy. Circulation, 112(8):1128-1135.

[12]Piao, H., Youn, T.J., Kwon, J.S., Kim, Y.H., Bae, J.W., Bora-Sohn, Kim, D.W., Cho, M.C., Lee, M.M., Park, Y.B., 2005. Effects of bone marrow derived mesenchymal stem cells transplantation in acutely infarcting myocardium. Eur. J. Heart Fail., 7(5):730-738.

[13]Pittenger, M.F., Martin, B.J., 2004. Mesenchymal stem cells and their potential as cardiac therapeutics. Circ. Res., 95(1):9-20.

[14]Ponte, A.L., Marais, E., Gallay, N., Langonne, A., Delorme, B., Herault, O., Charbord, P., Domenech, J., 2007. The in vitro migration capacity of human bone marrow mesenchymal stem cells: comparison of chemokine and growth factor chemotactic activities. Stem Cells, 25(7):1737-1745.

[15]Ren, J., Samson, W.K., Sowers, J.R., 1999. Insulin-like growth factor I as a cardiac hormone: physiological and pathophysiological implications in heart disease. J. Mol. Cell. Cardiol., 31(11):2049-2061.

[16]Sakimura, K., Matsumoto, T., Miyamoto, C., Osaki, M., Shindo, H., 2006. Effects of insulin-like growth factor I on transforming growth factor β1 induced chondrogenesis of synovium-derived mesenchymal stem cells cultured in a polyglycolic acid scaffold. Cells Tissues Organs, 183(2):55-61.

[17]Tang, Y.L., Zhao, Q., Qin, X., Shen, L., Cheng, L., Ge, J., Phillips, M.I., 2005. Paracrine action enhances the effects of autologous mesenchymal stem cell transplantation on vascular regeneration in rat model of myocardial infarction. Ann. Thorac. Surg., 80(1):229-236, 236-237.

[18]Tao, Z.W., Li, L.G., Geng, Z.H., Dang, T., Zhu, S.J., 2010. Growth factors induce the improved cardiac remodeling in autologous mesenchymal stem cell-implanted failing rat hearts. J. Zhejiang Univ.-Sci. B (Biomed. & Biotechnol.), 11(4):238-248.

[19]Tomita, S., Li, R.K., Weisel, R.D., Mickle, D.A., Kim, E.J., Sakai, T., Jia, Z.Q., 1999. Autologous transplantation of bone marrow cells improves damaged heart function. Circulation, 100(s19):I247-I256.

[20]Xiang, M.X., He, A.N., Wang, J.A., Gui, C., 2009. Protective paracrine effect of mesenchymal stem cells on cardiomyocytes. J. Zhejiang Univ.-Sci. B, 10(8):619-624.

[21]Xu, M., Uemura, R., Dai, Y., Wang, Y., Pasha, Z., Ashraf, M., 2007. In vitro and in vivo effects of bone marrow stem cells on cardiac structure and function. J. Mol. Cell. Cardiol., 42(2):441-448.

[22]Yang, J., Zhou, W., Zheng, W., Ma, Y., Lin, L., Tang, T., Liu, J., Yu, J., Zhou, X., Hu, J., 2007. Effects of myocardial transplantation of marrow mesenchymal stem cells transfected with vascular endothelial growth factor for the improvement of heart function and angiogenesis after myocardial infarction. Cardiology, 107(1):17-29.