Piper betle leaf extract enhances the cytotoxicity effect of 5-fluorouracil in inhibiting the growth of HT29 and HCT116 colon cancer cells
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Pek Leng Ng, Nor Fadilah Rajab, Sue Mian Then, Yasmin Anum Mohd Yusof, Wan Zurinah Wan Ngah, Kar Yong Pin, Mee Lee Looi. Piper betle leaf extract enhances the cytotoxicity effect of 5-fluorouracil in inhibiting the growth of HT29 and HCT116 colon cancer cells[J]. Journal of Zhejiang University Science B, 2014, 15(8): 692-700.
@article{title="Piper betle leaf extract enhances the cytotoxicity effect of 5-fluorouracil in inhibiting the growth of HT29 and HCT116 colon cancer cells",
author="Pek Leng Ng, Nor Fadilah Rajab, Sue Mian Then, Yasmin Anum Mohd Yusof, Wan Zurinah Wan Ngah, Kar Yong Pin, Mee Lee Looi",
journal="Journal of Zhejiang University Science B",
volume="15",
number="8",
pages="692-700",
year="2014",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1300303"
}
%0 Journal Article
%T Piper betle leaf extract enhances the cytotoxicity effect of 5-fluorouracil in inhibiting the growth of HT29 and HCT116 colon cancer cells
%A Pek Leng Ng
%A Nor Fadilah Rajab
%A Sue Mian Then
%A Yasmin Anum Mohd Yusof
%A Wan Zurinah Wan Ngah
%A Kar Yong Pin
%A Mee Lee Looi
%J Journal of Zhejiang University SCIENCE B
%V 15
%N 8
%P 692-700
%@ 1673-1581
%D 2014
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1300303
TY - JOUR
T1 - Piper betle leaf extract enhances the cytotoxicity effect of 5-fluorouracil in inhibiting the growth of HT29 and HCT116 colon cancer cells
A1 - Pek Leng Ng
A1 - Nor Fadilah Rajab
A1 - Sue Mian Then
A1 - Yasmin Anum Mohd Yusof
A1 - Wan Zurinah Wan Ngah
A1 - Kar Yong Pin
A1 - Mee Lee Looi
J0 - Journal of Zhejiang University Science B
VL - 15
IS - 8
SP - 692
EP - 700
%@ 1673-1581
Y1 - 2014
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1300303
Abstract: Objective: The combination effect of Piper betle (PB) and 5-Fluorouracil (5-FU) in enhancing the cytotoxic potential of 5-FU in inhibiting the growth of colon cancer cells was investigated. Methods: HT29 and HCT116 cells were subjected to 5-FU or PB treatment. 5-FU and PB were then combined and their effects on both cell lines were observed after 24 h of treatment. PB-5-FU interaction was elucidated by isobologram analysis. Apoptosis features of the treated cells were revealed by annexin V/PI stain. High-performance liquid chromatography (HPLC) was performed to exclude any possible chemical interaction between the compounds. Results: In the presence of PB extract, the cytotoxicity of 5-FU was observed at a lower dose (IC50 12.5 μmol/L) and a shorter time (24 h) in both cell lines. Both cell lines treated with 5-FU or PB alone induced a greater apoptosis effect compared with the combination treatment. isobologram analysis indicated that PB and 5-FU interacted synergistically and antagonistically in inhibiting the growth of HT29 and HCT116 cells, respectively. Conclusions: In the presence of PB, a lower dosage of 5-FU is required to achieve the maximum drug effect in inhibiting the growth of HT29 cells. However, PB did not significantly reduce 5-FU dosage in HCT116 cells. Our result showed that this interaction may not solely contribute to the apoptosis pathway.
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Summary: 
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CLC number: R979.1
On-line Access: 2024-08-27
Received: 2023-10-17
Revision Accepted: 2024-05-08
Crosschecked: 2014-07-11
Cited: 4
Clicked: 16399
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