JZUS - Journal of Zhejiang University SCIENCE

Journal of Zhejiang University SCIENCE B 2015 Vol.16 No.7 P.632-639

Correlation analysis of gene polymorphisms and β-lactam allergy

Author(s): Jing Li, Xin-yue Liu, Lin-jing Li, Chong-ge You, Lei Shi, Shang-di Zhang, Qian Liu, Jun Wang, Ze-jing Liu, Ting-hong Lv
Affiliation(s): Centre of Laboratory Medicine, Lanzhou University Second Hospital, Lanzhou 730030, China; more
Corresponding email(s): liuxy@lzu.edu.cn
Key Words: Allergy, β, -Lactam, Interleukin (IL), Pharmacogenomics, Single nucleotide polymorphism (SNP)

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Abstract: A total of 64 patients with β;-Lactam allergy and 30 control subjects were enrolled in a case-control study. This study is aimed to analyze the relationship between β;-Lactam allergy and 10 single nucleotide polymorphisms (SNPs) in interleukin-10 (IL-10), IL-13, IL-4Rα, high-affinity immunoglobulin E-receptor β; chain (FcεRIβ;), interferon γ receptor 2 (IFNGR2), and CYP3A4, and within the Han Chinese population of Northwest China. Genotyping for the SNPs was conducted using the Sequenom MassARRAY^® platform. SPSS 17.0 was employed to analyze the statistical data and SHEsis was used to perform the haplotype reconstruction and analyze linkage disequilibrium of SNPs of IL-10 and IL-13. The results showed that the genotype distribution of CYP3A4 rs2242480/CT differed significantly between case and control groups of males (P=0.022; odds ratio (OR)=0.167, 95% confidence interval (CI): 0.032–0.867). Further analysis showed that CCA, CCG, and TAA haplotypes of IL-10 had no significant correlation in patients with β;-Lactam allergy. The correlation between CCT and CAC haplotypes of IL-13 and β;-Lactam allergy needs to be further studied. The analysis did not reveal any differences in the distribution of others gene polymorphisms between cases and controls.

基因多态性与β-内酰胺类抗生素过敏的关联性分析

目的：探讨IL-10、IL-13、IL-4Rα、FcεRIβ、IFNGR2、CYP3A4基因多态性与中国西北地区汉族人群β-内酰胺类抗生素过敏易感性的关联性。
创新点：首次在甘肃地区汉族人群中探讨10个单核苷酸多态性位点与β-内酰胺类抗生素过敏易感性的关联性，为该地区人群β-内酰胺类抗生素过敏患者提供了基因组学水平的诊断数据。
方法：以β-内酰胺类抗生素过敏者为研究对象进行病例对照研究，采用Sequenom MassARRAY^®分子量阵列技术平台定制单核苷酸多态性（SNP）芯片检测10个单核苷酸多态性与甘肃地区汉族人群β-内酰胺类抗生素过敏易感性的关联性。应用SHEsis软件完成IL-10及IL-13的连锁不平衡分析及单倍型构建。
结论：CYP3A4 rs2242480基因多态性与男性患者β-内酰胺类抗生素过敏有显著的相关性（P=0.022；OR=0.167，95% CI：0.032-0.867）。IL-13的CCT及CAC单倍型与中国西北地区汉族β-内酰胺类抗生素过敏的相关性有待进一步研究。

关键词：过敏；β-内酰胺类抗生素；白细胞介素；药物基因组学；单核苷酸多态性

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基因多态性与β-内酰胺类抗生素过敏的关联性分析

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