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CLC number: Q75

On-line Access: 2016-08-04

Received: 2015-08-22

Revision Accepted: 2015-12-20

Crosschecked: 2016-07-13

Cited: 0

Clicked: 3416

Citations:  Bibtex RefMan EndNote GB/T7714

 ORCID:

Yue-jin Pu

http://orcid.org/0000-0003-3173-427X

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Journal of Zhejiang University SCIENCE B 2016 Vol.17 No.8 P.591-596

http://doi.org/10.1631/jzus.B1500199


Association of VIPR-1 gene polymorphisms and haplotypes with egg production in laying quails


Author(s):  Yue-jin Pu, Yan Wu, Xiao-juan Xu, Jin-ping Du, Yan-zhang Gong

Affiliation(s):  Key Laboratory of Agricultural Animal Genetics, Breeding, and Reproduction of the Ministry of Education, Huazhong Agricultural University, Wuhan 430070, China; more

Corresponding email(s):   puyuejin@yeah.net, poultry@mail.hzau.edu.cn

Key Words:  Laying quail, Haplotypes, VIPR-1 gene, Egg production traits


Yue-jin Pu, Yan Wu, Xiao-juan Xu, Jin-ping Du, Yan-zhang Gong. Association of VIPR-1 gene polymorphisms and haplotypes with egg production in laying quails[J]. Journal of Zhejiang University Science B, 2016, 17(8): 591-596.

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author="Yue-jin Pu, Yan Wu, Xiao-juan Xu, Jin-ping Du, Yan-zhang Gong",
journal="Journal of Zhejiang University Science B",
volume="17",
number="8",
pages="591-596",
year="2016",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1500199"
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%0 Journal Article
%T Association of VIPR-1 gene polymorphisms and haplotypes with egg production in laying quails
%A Yue-jin Pu
%A Yan Wu
%A Xiao-juan Xu
%A Jin-ping Du
%A Yan-zhang Gong
%J Journal of Zhejiang University SCIENCE B
%V 17
%N 8
%P 591-596
%@ 1673-1581
%D 2016
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1500199

TY - JOUR
T1 - Association of VIPR-1 gene polymorphisms and haplotypes with egg production in laying quails
A1 - Yue-jin Pu
A1 - Yan Wu
A1 - Xiao-juan Xu
A1 - Jin-ping Du
A1 - Yan-zhang Gong
J0 - Journal of Zhejiang University Science B
VL - 17
IS - 8
SP - 591
EP - 596
%@ 1673-1581
Y1 - 2016
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1500199


Abstract: 
The laying quail is a worldwide breed which exhibits high economic value. In our current study, the vasoactive intestinal peptide receptor-1 (VIPR-1) was selected as the candidate gene for identifying traits of egg production. A single nucleotide polymorphism (SNP) detection was performed in 443 individual quails, including 196 quails from the H line, 202 quails from the L line, and 45 wild quails. The SNPs were genotyped using a polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Two mutations (G373T, A313G) were detected in all the tested quail populations. The associated analysis showed that the SNP genotypes of the VIPR-1 gene were significantly linked with the egg weight of G373T and A313G in 398 quails. The quails with the genotype GG always exhibited the largest egg weight for the two mutations in the H and L lines. Linkage disequilibrium (LD) analysis indicated that G373T and A313G loci showed the weakest LD. Seven main diplotypes from the four main reconstructed haplotypes were observed, indicating a significant association of diplotypes with egg weight. Quails with the h1h2 (GGGT) diplotype always exhibited the smallest egg weight and largest egg number at 20 weeks of age. The overall results suggest that the alterations in quails may be linked with potential major loci or genes affecting reproductive traits.

VIPR-1基因多态性及其单倍型与蛋用鹌鹑产蛋性能的相关性研究

目的:蛋用鹌鹑VIPR-1基因单核苷酸多态性(SNP)研究。
创新点:首次开展蛋用鹌鹑VIPR-1基因多态性研究,发现了两个与蛋重相关的多态性位点。
方法:VIPR-1基因设计引物,进行聚合酶链式反应-限制片段长度多态性分析,获得基因突变位点。并通过关联性分析,研究基因多态性与繁殖性状之间的相关性。
结论:在个体数为443个(202只L个体、196只H个体和45只野生鹌鹑个体)的群体中发现两个突变位点(G373T和A313G),且两位点间存在微弱的连锁不平衡。相关性分析表明,SNP多态性与鹌鹑蛋重之间存在明显的相关性。组成的7个主要二倍型中,含有h1h2(GGGT)的个体总是表现为较小的蛋重及较大的产蛋数。

关键词:血管活性肠肽;多态性;单倍型;VIPR-1基因;产蛋性能相关性

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

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