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CLC number: Q75

On-line Access: 2016-08-04

Received: 2015-08-22

Revision Accepted: 2015-12-20

Crosschecked: 2016-07-13

Cited: 0

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Citations:  Bibtex RefMan EndNote GB/T7714


Yue-jin Pu


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Journal of Zhejiang University SCIENCE B 2016 Vol.17 No.8 P.591-596


Association of VIPR-1 gene polymorphisms and haplotypes with egg production in laying quails

Author(s):  Yue-jin Pu, Yan Wu, Xiao-juan Xu, Jin-ping Du, Yan-zhang Gong

Affiliation(s):  Key Laboratory of Agricultural Animal Genetics, Breeding, and Reproduction of the Ministry of Education, Huazhong Agricultural University, Wuhan 430070, China; more

Corresponding email(s):   puyuejin@yeah.net, poultry@mail.hzau.edu.cn

Key Words:  Laying quail, Haplotypes, VIPR-1 gene, Egg production traits

Yue-jin Pu, Yan Wu, Xiao-juan Xu, Jin-ping Du, Yan-zhang Gong. Association of VIPR-1 gene polymorphisms and haplotypes with egg production in laying quails[J]. Journal of Zhejiang University Science B, 2016, 17(8): 591-596.

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publisher="Zhejiang University Press & Springer",

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%T Association of VIPR-1 gene polymorphisms and haplotypes with egg production in laying quails
%A Yue-jin Pu
%A Yan Wu
%A Xiao-juan Xu
%A Jin-ping Du
%A Yan-zhang Gong
%J Journal of Zhejiang University SCIENCE B
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%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1500199

T1 - Association of VIPR-1 gene polymorphisms and haplotypes with egg production in laying quails
A1 - Yue-jin Pu
A1 - Yan Wu
A1 - Xiao-juan Xu
A1 - Jin-ping Du
A1 - Yan-zhang Gong
J0 - Journal of Zhejiang University Science B
VL - 17
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SP - 591
EP - 596
%@ 1673-1581
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PB - Zhejiang University Press & Springer
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DOI - 10.1631/jzus.B1500199

The laying quail is a worldwide breed which exhibits high economic value. In our current study, the vasoactive intestinal peptide receptor-1 (VIPR-1) was selected as the candidate gene for identifying traits of egg production. A single nucleotide polymorphism (SNP) detection was performed in 443 individual quails, including 196 quails from the H line, 202 quails from the L line, and 45 wild quails. The SNPs were genotyped using a polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Two mutations (G373T, A313G) were detected in all the tested quail populations. The associated analysis showed that the SNP genotypes of the VIPR-1 gene were significantly linked with the egg weight of G373T and A313G in 398 quails. The quails with the genotype GG always exhibited the largest egg weight for the two mutations in the H and L lines. Linkage disequilibrium (LD) analysis indicated that G373T and A313G loci showed the weakest LD. Seven main diplotypes from the four main reconstructed haplotypes were observed, indicating a significant association of diplotypes with egg weight. Quails with the h1h2 (GGGT) diplotype always exhibited the smallest egg weight and largest egg number at 20 weeks of age. The overall results suggest that the alterations in quails may be linked with potential major loci or genes affecting reproductive traits.




Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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