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Journal of Zhejiang University SCIENCE A 2003 Vol.4 No.2 P.232-235


Purification and relationship with gastric disease of a 130 kDa (CagA) protein of Helicobacter pylori

Author(s):  YE Shao-jing, FANG Ping-chu, MAO Guo-gen, LI Chao-long, Qiu Xiang, CHEN Hai-xiang

Affiliation(s):  Department of Microbiology, College of Medicine, Zhejiang University, Hangzhou 310006, China

Corresponding email(s):   yesj@zju.edu.cn

Key Words:  Helicobacter pylori, CagA, Elution from gel, Western-blot

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YE Shao-jing, FANG Ping-chu, MAO Guo-gen, LI Chao-long, Qiu Xiang, CHEN Hai-xiang. Purification and relationship with gastric disease of a 130 kDa (CagA) protein of Helicobacter pylori[J]. Journal of Zhejiang University Science A, 2003, 4(2): 232-235.

@article{title="Purification and relationship with gastric disease of a 130 kDa (CagA) protein of Helicobacter pylori",
author="YE Shao-jing, FANG Ping-chu, MAO Guo-gen, LI Chao-long, Qiu Xiang, CHEN Hai-xiang",
journal="Journal of Zhejiang University Science A",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Purification and relationship with gastric disease of a 130 kDa (CagA) protein of Helicobacter pylori
%A YE Shao-jing
%A FANG Ping-chu
%A MAO Guo-gen
%A LI Chao-long
%A Qiu Xiang
%A CHEN Hai-xiang
%J Journal of Zhejiang University SCIENCE A
%V 4
%N 2
%P 232-235
%@ 1869-1951
%D 2003
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.2003.0232

T1 - Purification and relationship with gastric disease of a 130 kDa (CagA) protein of Helicobacter pylori
A1 - YE Shao-jing
A1 - FANG Ping-chu
A1 - MAO Guo-gen
A1 - LI Chao-long
A1 - Qiu Xiang
A1 - CHEN Hai-xiang
J0 - Journal of Zhejiang University Science A
VL - 4
IS - 2
SP - 232
EP - 235
%@ 1869-1951
Y1 - 2003
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.2003.0232

Objective: The aims of this research were to purify and identify the 130 kDa (cagA) protein of H. pylori clinical isolate HP97002 and evaluate the relationships between the purified 130 kDa (cagA) protein and gastric diseases. Methods: The procedure for isolating the protein included 6 mol/L guanidine extract, size exclusion chromatography and elusion from gel. Sera of 68 patients with gastric diseases (44 with chronic gastritis, 15 with atrophic gastritis, 7 with peptic ulcer disease, 2 with gastric cancer) were obtained, and the serological response to cagA was studied by western-blot using the purified protein. Results: The purified protein was 130 kDa and preserved good antigenicity and revealed basic isoelectric point about of 8.1. Among 68 sera, 43 sera could recognize the purified protein associated with chronic gastritis 47.7% (21/44),atrophic gastritis 86.7% (13/15), peptic ulcer disease 100% (7/7),gastric cancer 100% (2/2). Compared with each other, the difference was significant (χ2=13.327, P=0.004), and 130 kDa (cagA) protein was associated with severe gastric diseases (rs=0.442, P=0.001). Conclusion: The 130 kDa (cagA) protein was associated with severe gastric diseases.

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