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CLC number: R284.2

On-line Access: 2017-08-08

Received: 2016-05-24

Revision Accepted: 2016-09-12

Crosschecked: 2017-07-19

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Citations:  Bibtex RefMan EndNote GB/T7714


Yun Chen


Xiu-ju Du


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Journal of Zhejiang University SCIENCE B 2017 Vol.18 No.8 P.674-684


Ultrasound extraction optimization, structural features, and antioxidant activity of polysaccharides from Tricholoma matsutake

Author(s):  Yun Chen, Xiu-ju Du, Yang Zhang, Xin-hua Liu, Xuan-dong Wang

Affiliation(s):  College of Life Science, Liaocheng University, Liaocheng 252059, China; more

Corresponding email(s):   duxiuju@lcu.edu.cn

Key Words:  Tricholoma matsutake polysaccharide, Orthogonal test design, Ultrasound-assisted extraction, Monosaccharide composition, Antioxidant activity

Yun Chen, Xiu-ju Du, Yang Zhang, Xin-hua Liu, Xuan-dong Wang. Ultrasound extraction optimization, structural features, and antioxidant activity of polysaccharides from Tricholoma matsutake[J]. Journal of Zhejiang University Science B, 2017, 18(8): 674-684.

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author="Yun Chen, Xiu-ju Du, Yang Zhang, Xin-hua Liu, Xuan-dong Wang",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Ultrasound extraction optimization, structural features, and antioxidant activity of polysaccharides from Tricholoma matsutake
%A Yun Chen
%A Xiu-ju Du
%A Yang Zhang
%A Xin-hua Liu
%A Xuan-dong Wang
%J Journal of Zhejiang University SCIENCE B
%V 18
%N 8
%P 674-684
%@ 1673-1581
%D 2017
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1600239

T1 - Ultrasound extraction optimization, structural features, and antioxidant activity of polysaccharides from Tricholoma matsutake
A1 - Yun Chen
A1 - Xiu-ju Du
A1 - Yang Zhang
A1 - Xin-hua Liu
A1 - Xuan-dong Wang
J0 - Journal of Zhejiang University Science B
VL - 18
IS - 8
SP - 674
EP - 684
%@ 1673-1581
Y1 - 2017
PB - Zhejiang University Press & Springer
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DOI - 10.1631/jzus.B1600239

An ultrasonic-assisted technique was employed to extract crude polysaccharide from Tricholoma matsutake fruiting bodies. Single-factor tests and orthogonal experimental design (L9(33)) were used to obtain the optimal extraction conditions. Results showed that the optimal parameters were as follows: ultrasonic temperature, 40 °C; ultrasonic time, 50 min; water to raw material ratio, 25 ml/g; ultrasonic frequency, 45 kHz; and ultrasonic power, 100 W. Three novel T. matsutake polysaccharide (TMP) fractions (TMP30, TMP60, and TMP80) were isolated and purified from TMP by stepwise alcohol precipitation. Their preliminary structural features were determined by high-performance anion-exchange chromatography with pulsed-amperometric detection (HPAEC-PAD) and Fourier transform infrared spectrophotometer (FT-IR) analyses. Furthermore, their in vitro antioxidant activity was investigated in terms of a reducing power assay and the scavenging rates of 2,2-diphenyl-1-picrylhydrazyl (DPPH) and hydroxyl radicals. The order of the various fractions based on their antioxidant activity was TMP80>TMP>TMP60>TMP30. These findings suggested that novel polysaccharide fractions from T. matsutake, especially TMP80, could be promising active macromolecules for biomedical use.


结论:正交试验结果表明,超声提取TMP的最优工艺条件为:超声温度40 °C,超声时间50 min,水原料比例25 ml/g,超声波频率45 kHz和超声波功率100 W(表1和表2)。在此条件下的TMP得率为8.06%。采用乙醇分级法对TMP进一步分离纯化,制备得到3种多糖TMP30、TMP60和TMP80。HPAEC-PAD和FT-IR的结果表明,TMP30、TMP60和TMP80均主要由岩藻糖(L-Fuc)、半乳糖(D-Gal)、葡萄糖(D-Glc)、木糖(D-Xyl)和甘露糖(D-Man)组成,它们具有相同的单糖组成,但含有不同的摩尔比,且均有β-构型(图2、图3和表3)。抗氧化活性结果表明,它们的能力高低顺序为:TMP80>TMP>TMP60>TMP30(图4和表4),TMP80抗氧化活性最高,为松茸多糖的进一步开发利用提供重要的科学依据。


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