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CLC number: R331.1

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Received: 2005-07-25

Revision Accepted: 2005-11-03

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Journal of Zhejiang University SCIENCE B 2005 Vol.6 No.12 P.1148-1152


Determination of theophylline concentration in serum by chemiluminescent immunoassay

Author(s):  ZHOU Mei-xia, GUAN Cha-ying, CHEN Guang, XIE Xin-you, WU Sheng-hai

Affiliation(s):  Department of Laboratory, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou 310016, China; more

Corresponding email(s):   zmx00018@126.com

Key Words:  Theophylline, Chemiluminescent immunoassay (CLIA), Fluorescence polarization immunoassay (FPIA)

ZHOU Mei-xia, GUAN Cha-ying, CHEN Guang, XIE Xin-you, WU Sheng-hai. Determination of theophylline concentration in serum by chemiluminescent immunoassay[J]. Journal of Zhejiang University Science B, 2005, 6(12): 1148-1152.

@article{title="Determination of theophylline concentration in serum by chemiluminescent immunoassay",
author="ZHOU Mei-xia, GUAN Cha-ying, CHEN Guang, XIE Xin-you, WU Sheng-hai",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Determination of theophylline concentration in serum by chemiluminescent immunoassay
%A ZHOU Mei-xia
%A GUAN Cha-ying
%A CHEN Guang
%A XIE Xin-you
%A WU Sheng-hai
%J Journal of Zhejiang University SCIENCE B
%V 6
%N 12
%P 1148-1152
%@ 1673-1581
%D 2005
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.2005.B1148

T1 - Determination of theophylline concentration in serum by chemiluminescent immunoassay
A1 - ZHOU Mei-xia
A1 - GUAN Cha-ying
A1 - CHEN Guang
A1 - XIE Xin-you
A1 - WU Sheng-hai
J0 - Journal of Zhejiang University Science B
VL - 6
IS - 12
SP - 1148
EP - 1152
%@ 1673-1581
Y1 - 2005
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.2005.B1148

Objective: This study aimed to establish chemiluminescent immunoassay (CLIA) for quantitative determination of theophylline levels in human serum. Methods: To measure the concentration of theophylline (n=122) and evaluate the assay. Results: The linear range of the CLIA method was 0.51~40 mg/L (Y=1.02X+0.44, r=0.995). The intra and inter CV (coefficient variance) of CLIA were 3.20% and 3.57%, respectively. The average recovery rate was 102.3%. This method was free from interference by brilirubin (<200 μmol/L), hemoglobin (<10 g/L), and triglycerides (<15 mmol/L). Conclusion: This method is simple, convenient and precise for clinical pharmacokinetics study of theophylline.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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